Localisation of the genomic sequence interval for the blue eggshell gene using an F2 resource population of Dongxiang chickens

Wang, X.-T.; Bai, Jirong; Zhao, Chunjiang; Zhang, H.; Bao, Huar; Xu, Guiyun; Li, J.-Y.; Lin-sheng, Lian; Wu, Changxin; Deng, Xuemei

doi:10.1080/00071668.2010.502520

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…In the region around ev1 , two single nucleotide polymorphisms (SNPs) (rs15297163 and rs15297165) were found to be highly associated with the blue eggshell phenotype [18]. A 1.8 Mb genomic interval harboring the O gene was defined in an F 2 resource population [19]. The localization of the O was further refined to the vicinity of ss244244378 by linkage and association analysis [20].…”

Section: Introductionmentioning

confidence: 99%

An EAV-HP Insertion in 5′ Flanking Region of SLCO1B3 Causes Blue Eggshell in the Chicken

et al. 2013

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The genetic determination of eggshell coloration has not been determined in birds. Here we report that the blue eggshell is caused by an EAV-HP insertion that promotes the expression of SLCO1B3 gene in the uterus (shell gland) of the oviduct in chicken. In this study, the genetic map location of the blue eggshell gene was refined by linkage analysis in an F2 chicken population, and four candidate genes within the refined interval were subsequently tested for their expression levels in the shell gland of the uterus from blue-shelled and non-blue-shelled hens. SLCO1B3 gene was found to be the only one expressed in the uterus of blue-shelled hens but not in that of non-blue-shelled hens. Results from a pyrosequencing analysis showed that only the allele of SLCO1B3 from blue-shelled chickens was expressed in the uterus of heterozygous hens (O*LC/O*N). SLCO1B3 gene belongs to the organic anion transporting polypeptide (OATP) family; and the OATPs, functioning as membrane transporters, have been reported for the transportation of amphipathic organic compounds, including bile salt in mammals. We subsequently resequenced the whole genomic region of SLCO1B3 and discovered an EAV-HP insertion in the 5′ flanking region of SLCO1B3. The EAV-HP insertion was found closely associated with blue eggshell phenotype following complete Mendelian segregation. In situ hybridization also demonstrated that the blue eggshell is associated with ectopic expression of SLCO1B3 in shell glands of uterus. Our finding strongly suggests that the EAV-HP insertion is the causative mutation for the blue eggshell phenotype. The insertion was also found in another Chinese blue-shelled breed and an American blue-shelled breed. In addition, we found that the insertion site in the blue-shelled chickens from Araucana is different from that in Chinese breeds, which implied independent integration events in the blue-shelled chickens from the two continents, providing a parallel evolutionary example at the molecular level.

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Section: Introductionmentioning

confidence: 99%

An EAV-HP Insertion in 5′ Flanking Region of SLCO1B3 Causes Blue Eggshell in the Chicken

et al. 2013

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“…It gives rise to a blue or green eggshell colour depending on whether the base colour is white or brown. It has recently been mapped to a broad region on Gga 1 (67.3-69.1 Mb) in the Dongxiang chicken breed (Wang et al, 2010). The rose comb (R) is an autosomal dominant trait, which when combined with pea comb results in walnut comb.…”

Section: Phenotypic Traitsmentioning

confidence: 99%

Analysis of genome-wide structure, diversity and fine mapping of Mendelian traits in traditional and village chickens

et al. 2012

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Extensive phenotypic variation is a common feature among village chickens found throughout much of the developing world, and in traditional chicken breeds that have been artificially selected for traits such as plumage variety. We present here an assessment of traditional and village chicken populations, for fine mapping of Mendelian traits using genome-wide singlenucleotide polymorphism (SNP) genotyping while providing information on their genetic structure and diversity. Bayesian clustering analysis reveals two main genetic backgrounds in traditional breeds, Kenyan, Ethiopian and Chilean village chickens. Analysis of linkage disequilibrium (LD) reveals useful LD (r 2 X0.3) in both traditional and village chickens at pairwise marker distances of B10 Kb; while haplotype block analysis indicates a median block size of 11-12 Kb. Association mapping yielded refined mapping intervals for duplex comb (Gga 2:38.55-38.89 Mb) and rose comb (Gga 7:18.41-22.09 Mb) phenotypes in traditional breeds. Combined mapping information from traditional breeds and Chilean village chicken allows the oocyan phenotype to be fine mapped to two small regions (Gga 1:67.25-67.28 Mb, Gga 1:67.28-67.32 Mb) totalling B75 Kb. Mapping the unmapped earlobe pigmentation phenotype supports previous findings that the trait is sex-linked and polygenic. A critical assessment of the number of SNPs required to map simple traits indicate that between 90 and 110K SNPs are required for full genome-wide analysis of haplotype block structure/ancestry, and for association mapping in both traditional and village chickens. Our results demonstrate the importance and uniqueness of phenotypic diversity and genetic structure of traditional chicken breeds for fine-scale mapping of Mendelian traits in the species, with village chicken populations providing further opportunities to enhance mapping resolutions.

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“…Os ovos de casca marrom contêm grandes quantidades de protoporfirina IX e quantidades relativamente pequenas de biliverdina-IX (Kennedy e Vevers, 1976;Lang e Wells, 1987;. Estes pigmentos são produzidos no útero e depositados na casca do ovo no final da sua formação (Baird et al, 1975;Lang e Wells, 1987;Li et al, 2013;Wang et al, 2007;Wang et al, 2010). O pigmento é depositado em todas as camadas da casca, incluindo as membranas, mas a maior parte está concentrada na camada mais externa da casca e na cutícula .…”

Section: Cor Da Cascaunclassified

“…As protoporfirinas são um precursor imediato da molécula heme (Liu e Cheng, 2010) e conferem uma cor avermelhada ou marrom à casca do ovo (Kennedy e Vevers, 1976, Wang et al, 2011 Os ovos de casca azul contêm protoporfirina IX, biliverdina e seu quelato de zinco e vestígios de coproporfirina (Kennedy e Vevers, 1973). A biliverdina é um pigmento verde, subproduto do metabolismo da hemoglobina (Liu e Cheng, 2010) e os ovos que contêm este pigmento apresentam casca de cor azul, verde ou variações destas tonalidades (Punnett, 1933, Wang et al, 2010. Nos mamíferos, a biliverdina é convertida em bilirrubina pela enzima biliverdina redutase, no entanto, nas aves esta enzima tem uma baixa atividade, o que torna a biliverdina a principal forma de excreção (Wang et al, 2011).…”

Section: Cor Da Cascaunclassified

Parâmetros genéticos de características de produção e qualidade de ovos em linhagens nacionais de galinhas caipiras

Bogdanski¹

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Localisation of the genomic sequence interval for the blue eggshell gene using an F2 resource population of Dongxiang chickens

Cited by 4 publications

References 6 publications

An EAV-HP Insertion in 5′ Flanking Region of SLCO1B3 Causes Blue Eggshell in the Chicken

An EAV-HP Insertion in 5′ Flanking Region of SLCO1B3 Causes Blue Eggshell in the Chicken

Analysis of genome-wide structure, diversity and fine mapping of Mendelian traits in traditional and village chickens

Parâmetros genéticos de características de produção e qualidade de ovos em linhagens nacionais de galinhas caipiras

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