Localization and Characterization of 170 BAC-Derived Clones and Mapping of 94 Microsatellites in the Hessian Fly

Schemerhorn, Brandon J.; Crane, Yan M.; Morton, Philip K.; Aggarwal, Rajat; Benatti, Thiago Romanos

doi:10.1093/jhered/esp045

Cited by 7 publications

(16 citation statements)

References 24 publications

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“…This result was the same for the North American populations (both in K7 and K8), as well as the Old World populations. Both loci under selection were located on chromosome A1; however, Hf119 is located on the short arm and Hf174 on the long arm [29].…”

Section: Resultsmentioning

confidence: 99%

“…Sex was not determined for pupal samples, and thus disregarded in any analyses. Eighteen polymorphic microsatellite loci of varying di-, tri-, and one tetra-nucleotide motifs included loci from all chromosomes, based on physical location from hybridization to polytene chromosomes [29] (Table 2). Due to the hemizygous nature of sex chromosomes in males [30] and the possibility of artificially increasing observed homozygosity, only three sex-linked loci were used.…”

Section: Methodsmentioning

confidence: 99%

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Population Structure and the Colonization Route of One of the Oldest North American Invasive Insects: Stories from the Worn Road of the Hessian Fly, Mayetiola destructor (Say)

Morton

Schemerhorn

2013

PLoS ONE

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An integral part to understanding the biology of an invasive species is determining its origin, particularly in pest species. As one of the oldest known invasive species, the goals of this study were to evaluate the evidence of a westward expansion of Hessian fly into North America, from a potential singular introduction event, and the population genetic structure of current populations. Levels of genetic diversity and population structure in the Hessian fly were compared across North America, Europe, North Africa, Western Asia, and New Zealand. Furthermore, Old World populations were evaluated as possible sources of introduction. We tested diversity and population structure by examining 18 microsatellite loci with coverage across all four Hessian fly chromosomes. Neither genetic diversity nor population genetic structure provided evidence of a westward movement from a single introduction in North America. Introduced populations in North America did not show identity or assignment to any Old World population, likely indicating a multiple introduction scenario with subsequent gene flow between populations. Diversity and selection were assessed on a chromosomal level, with no differences in diversity or selection between chromosomes or between native and introduced populations.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Population Structure and the Colonization Route of One of the Oldest North American Invasive Insects: Stories from the Worn Road of the Hessian Fly, Mayetiola destructor (Say)

Morton

Schemerhorn

2013

PLoS ONE

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“…The eluted DNA samples were divided into two stocks and stored at Ϫ20ЊC and Ϫ80ЊC for short-and long-term storage, respectively. Fourteen polymorphic microsatellite loci of varying di-, tri-, and one tetra-nucleotide motifs were selected to give widespread coverage across the chromosomal arms of the polytene chromosomes, including two sex-linked loci (Schemerhorn et al 2009). Some primers were altered to increase the number of loci in pool-plexing, which is the combining of loci during genotyping (Table 1).…”

Section: Methodsmentioning

confidence: 99%

Population Structure and Spatial Influence of Agricultural Variables on Hessian Fly Populations in the Southeastern United States

2011

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Population structure dictates the evolution of each population, and thus, the species as a whole. Incorporating spatial variables with population genetic statistics allows for greater discovery beyond traditional population genetics alone and can inform management decisions. The understanding of population structure in Hessian fly, Mayetiola destructor (Say), a pest of wheat, has been limited in the past. We scored 14 microsatellite loci from 12 collections of Hessian fly in the southeastern United States. Through Bayesian clustering analysis, we found two major populations of Hessian fly covering the entire southeastern United States. We evaluated correlations between agriculturally significant spatial variables and population genetic differentiation to test if genetic structure has an ecological component in a wheat agro-ecosystem. Our results suggest the total amount of alternative host plants in the county may be driving some genetic differentiation. Although planting date may also be influential, geographic distance, mean annual temperature, and harvested wheat for grain do not seem to be contributing factors. The ecological or spatial component to population structure, however, may be minimal compared to factors such as genetic drift.

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“…To determine recombination frequencies along the long arm of chromosome A1, individual Hessian flies were genotyped for alleles segregating for three sequence tagged sites (STSs) and two simple sequence repeat (SSR) markers (supporting information, Figure S1) that had been discovered previously in BAC sequence data Schemerhorn et al 2009). Each individual was genotyped using its DNA as a template in separate PCRs.…”

Section: Methodsmentioning

confidence: 99%

A Neo-Sex Chromosome That Drives Postzygotic Sex Determination in the Hessian Fly (Mayetiola destructor)

et al. 2010

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Two nonoverlapping autosomal inversions defined unusual neo-sex chromosomes in the Hessian fly (Mayetiola destructor). Like other neo-sex chromosomes, these were normally heterozygous, present only in one sex, and suppressed recombination around a sex-determining master switch. Their unusual properties originated from the anomalous Hessian fly sex determination system in which postzygotic chromosome elimination is used to establish the sex-determining karyotypes. This system permitted the evolution of a master switch (Chromosome maintenance, Cm) that acts maternally. All of the offspring of females that carry Cm-associated neo-sex chromosomes attain a female-determining somatic karyotype and develop as females. Thus, the chromosomes act as maternal effect neo-W's, or W-prime (W9) chromosomes, where ZW9 females mate with ZZ males to engender female-producing (ZW9) and maleproducing (ZZ) females in equal numbers. Genetic mapping and physical mapping identified the inversions. Their distribution was determined in nine populations. Experimental matings established the association of the inversions with Cm and measured their recombination suppression. The inversions are the functional equivalent of the sciarid X-prime chromosomes. We speculate that W9 chromosomes exist in a variety of species that produce unisexual broods.

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Localization and Characterization of 170 BAC-Derived Clones and Mapping of 94 Microsatellites in the Hessian Fly

Cited by 7 publications

References 24 publications

Population Structure and the Colonization Route of One of the Oldest North American Invasive Insects: Stories from the Worn Road of the Hessian Fly, Mayetiola destructor (Say)

Population Structure and the Colonization Route of One of the Oldest North American Invasive Insects: Stories from the Worn Road of the Hessian Fly, Mayetiola destructor (Say)

Population Structure and Spatial Influence of Agricultural Variables on Hessian Fly Populations in the Southeastern United States

A Neo-Sex Chromosome That Drives Postzygotic Sex Determination in the Hessian Fly (Mayetiola destructor)

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