Localization and neuronal response of RFamide related peptides in the rat central nervous system

Yano, Takahiko; Iijima, Norio; Kakihara, Kenshi; Hinuma, Shuji; Tanaka, Masaki; Ibata, Yasuhiko

doi:10.1016/s0006-8993(03)02877-4

Cited by 104 publications

(114 citation statements)

References 30 publications

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“…Although we cannot rule out the presence RFRP-3 in the portal blood from other sources than the median eminence, our observations suggest that RFRP-3 is a direct inhibitor of GnRH neurons. Our study observed a greater distribution of cell bodies than observed by other studies in the rat (Yano et al, 2003). We confirmed the specificity of our antibody with preabsorption studies both with and without the antigen retrieval protocols; however, we cannot discount the possibility of cross reactivity with another closely related RFRP peptides.…”

Section: Discussionsupporting

confidence: 83%

“…Large numbers of cell bodies containing RFRP-3-ir were found in both the dorsal and ventral components of the dorsomedial hypothalamic nucleus (DMH, essentially surrounding the VMN) and the dorsal tuberomammillary nucleus (TMN). This distribution of RFRP-3 is similar to that reported previously (Yano, Iijima, Hinuma, Tanaka, and Ibata, 2004;Yano et al, 2003). Yano et al (Yano et al, 2004;Yano et al, 2003) suggested that both RFRP-1 (quail RFRP-3) and RFRP-3 (rat RFRP-3) were found in the rat hypothalamus, however no specificity data was presented for either of the two antisera used in their experiments-given the high degree of similarity in tertiary structure between these two small peptides (12 AA vs 16 AA, respectively) it is possible a great deal of cross-reactivity occurred in that study.…”

Section: Experiments 2: Immunocytochemistry For Rfrp-3supporting

confidence: 90%

“…This distribution of RFRP-3 is similar to that reported previously (Yano, Iijima, Hinuma, Tanaka, and Ibata, 2004;Yano et al, 2003). Yano et al (Yano et al, 2004;Yano et al, 2003) suggested that both RFRP-1 (quail RFRP-3) and RFRP-3 (rat RFRP-3) were found in the rat hypothalamus, however no specificity data was presented for either of the two antisera used in their experiments-given the high degree of similarity in tertiary structure between these two small peptides (12 AA vs 16 AA, respectively) it is possible a great deal of cross-reactivity occurred in that study. We are aware that our antisera for rat RFRP-3 does indeed cross react with quail RFRP-3 (RFRP-1, (Ukena et al, 2002)).…”

Section: Experiments 2: Immunocytochemistry For Rfrp-3supporting

confidence: 90%

“…Furthermore, double-label immunocytochemistry (ICC) revealed a high probability that GnIH terminals were in close contact with GnRH neurons and fibers (Bentley et al, 2003). Recently, another members of this family (RFRP-1 & RFRP-3) were isolated and sequenced in the rat and found to be widely expressed in the rat hypothalamus (Hinuma, Shintani, Fukusumi, Iijima, Matsumoto, Hosoya, Fujii, Watanabe, Kikuchi, Terao, Yano, Yamamoto, Kawamata, Habata, Asada, Kitada, Kurokawa, Onda, Nishimura, Tanaka, Ibata, and Fujino, 2000;Ukena, Iwakoshi, Minakata, and Tsutsui, 2002;Yano, Iijima, Kakihara, Hinuma, Tanaka, and Ibata, 2003). Although structurally similar peptides, the gene sequence encoding avian GnIH is missing in the homologous mammalian gene (Hinuma, Habata, Fujii, Kawamata, Hosoya, Fukusumi, Kitada, Masuo, Asano, Matsumoto, Sekiguchi, Kurokawa, Nishimura, Onda, and Fujino, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Rat RFamide-related peptide-3 stimulates GH secretion, inhibits LH secretion, and has variable effects on sex behavior in the adult male rat

Johnson

Tsutsui

Fraley

2007

Hormones and Behavior

352

383

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A recently described avian neuropeptide, gonadotropin inhibitory hormone (GnIH), has been shown to have seasonal regulatory effects on the hypothalamic-pituitary-gonadotropin axis (HPG) in several avian species. In the bird, GnIH expression is increased during the photorefractory period and has inhibitory effects on the HPG. A recently described mammalian neuropeptide, RF-amide-related peptide-3 (RFRP-3), may be genetically related and functionally similar to this avian neuropeptide. The purposes of this study were to first see if rat RFRP-3 is expressed in the male rat brain and second to determine if ICV injections of RFRP-3 will have effects on feeding and sex behaviors, as well as hormone release from the anterior pituitary. Results confirm other studies in that immunoreactive cell bodies and fibers are observable in areas of the male rat brain known to control the HPG and feeding and sex behaviors. RFRP-3 fibers are also observed in close proximity to GnRH immunoreactive cell bodies. Behavioral tests indicate that high but not low ICV RFRP-3 (500 vs. 100 ng, respectively) significantly (p < 0.05) suppressed all facets of male sex behavior while not having any observable effects on their ability to ambulate. Sex behavior was later exhibited when those same male rats received the ICV vehicle. While suppressing sex behavior, ICV RFRP-3 significantly (p < 0.05) increased food intake compared to controls. ICV RFRP-3 also significantly reduced plasma levels of luteinizing hormone but increased growth hormone regardless of the time of day; however, at no time did RFRP-3 alter plasma levels of FSH, thyroid hormone, or cortisol. These results indicate that although RFRP-3 has similar effects on LH as observed with GnIH in avian species, in the rat RFRP-3 has additional roles in regulating feeding and growth.

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Section: Discussionsupporting

confidence: 83%

Section: Experiments 2: Immunocytochemistry For Rfrp-3supporting

confidence: 90%

Section: Experiments 2: Immunocytochemistry For Rfrp-3supporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rat RFamide-related peptide-3 stimulates GH secretion, inhibits LH secretion, and has variable effects on sex behavior in the adult male rat

Johnson

Tsutsui

Fraley

2007

Hormones and Behavior

352

383

View full text Add to dashboard Cite

show abstract

“…Studies of the rat brain imply that neurons expressing RFRP-1 and -3 are limited to the caudal portion of the hypothalamus, namely the periventricular nucleus (PeVN), and an area between the VMN and the DMN , Fukusumi et al 2001, Yano et al 2003, although it is unclear from these reports whether RFRP-expressing neurons can be found elsewhere. As with the mouse, RFRP immunoreactive nerve fibres are widespread in the rat brain, but notably include the hypothalamic PVN, the supraoptic nucleus (SON) and the external layer of the dorsal horn (Fukusumi et al 2001, Yano et al 2003. ICV administration of the RFRPs induce the expression of c-fos in a number of areas, including the anterior NTS, the locus coeruleus, SON, PeVN and the arcuate nucleus of the hypothalamus (Yano et al 2003).…”

Section: Lpxrfamide Familymentioning

confidence: 99%

The role of RFamide peptides in feeding

Bechtold

Luckman

2007

Journal of Endocrinology

118

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In the three decades since FMRFamide was isolated from the clam Macrocallista nimbosa, the list of RFamide peptides has been steadily growing. These peptides occur widely across the animal kingdom, including five groups of RFamide peptides identified in mammals. Although there is tremendous diversity in structure and biological activity in the RFamides, the involvement of these peptides in the regulation of energy balance and feeding behaviour appears consistently through evolution. Even so, questions remain as to whether feedingrelated actions represent a primary function of the RFamides, especially within mammals. However, as we will discuss here, the study of RFamide function is rapidly expanding and with it so is our understanding of how these peptides can influence food intake directly as well as related aspects of feeding behaviour and energy expenditure.

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The hypothalamic neuropeptide FF network is impaired in hypertensive patients

et al. 2014

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BackgroundThe human hypothalamus contains the neuropeptide FF (NPFF) neurochemical network. Animal experiments demonstrated that NPFF is implicated in the central cardiovascular regulation. We therefore studied expression of this peptide in the hypothalamus of individuals who suffered from essential hypertension (n = 8) and died suddenly due to acute myocardial infarction (AMI), and compared to that of healthy individuals (controls) (n = 6) who died abruptly due to mechanical trauma of the chest.MethodsThe frozen right part of the hypothalamus was cut coronally into serial sections of 20 μm thickness, and each tenth section was stained immunohistochemically using antibody against NPFF. The central section through each hypothalamic nucleus was characterized by the highest intensity of NPFF immunostaining and thus was chosen for quantitative densitometry.ResultsIn hypertensive patients, the area occupied by NPFF immunostained neuronal elements in the central sections through the suprachiasmatic nucleus (SCh), paraventricular hypothalamic nucleus (Pa), bed nucleus of the stria terminalis (BST), perinuclear zone (PNZ) of the supraoptic nucleus (SON), dorso- (DMH), ventromedial (VMH) nuclei, and perifornical nucleus (PeF) was dramatically decreased compared to controls, ranging about six times less in the VMH to 15 times less in the central part of the BST (BSTC). The NPFF innervation of both nonstained neuronal profiles and microvasculature was extremely poor in hypertensive patients compared to control.ConclusionsThe decreased NPFF expression in the hypothalamus of hypertensive patients might be a cause of impairment of its interaction with other neurochemical systems, and thereby might be involved in the pathogenesis of the disease.

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Localization and neuronal response of RFamide related peptides in the rat central nervous system

Cited by 104 publications

References 30 publications

Rat RFamide-related peptide-3 stimulates GH secretion, inhibits LH secretion, and has variable effects on sex behavior in the adult male rat

Rat RFamide-related peptide-3 stimulates GH secretion, inhibits LH secretion, and has variable effects on sex behavior in the adult male rat

The role of RFamide peptides in feeding

The hypothalamic neuropeptide FF network is impaired in hypertensive patients

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