Localization and osteoblastic differentiation potential of neural crest-derived cells in oral tissues of adult mice

Ono, Miki; Suzawa, Tetsuo; Miki, Takami; Yamamoto, Gou; Hosono, Tomohiko; Yamada, Atsushi; Suzuki, Daisuke; Yoshimura, Kenichi; Watahiki, Junichi; Hayashi, Ryuhei; Arata, Satoru; Mishima, Kenji; Nishida, Kohji; Osumi, Noriko; Maki, Koutaro; Kamijo, Ryutaro

doi:10.1016/j.bbrc.2015.07.106

Cited by 13 publications

(13 citation statements)

References 31 publications

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“…The P0-Cre transgenic mouse line has been widely used for NC cell fate mapping (Feltri et al, 1999b;Ono et al, 2015;Sommer and Suter, 1998) and genetic modifications of NC-derived cells (Hu, Strobl-Mazzulla, & Bronner, 2014;Liu and Xiao, 2011). However, questions remain about how early, how specifically, and to what extent P0-Cre transgene labels NC cells in early embryos.…”

Section: Discussionmentioning

confidence: 99%

Specific and spatial labeling of P0‐Cre versus Wnt1‐Cre in cranial neural crest in early mouse embryos

Chen

Ishan

Yang

et al. 2017

Genesis

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P0-Cre and Wnt1-Cre mouse lines have been widely used in combination with loxP-flanked mice to label and genetically modify neural crest (NC) cells and their derivatives. Wnt1-Cre has been regarded as the gold standard and there have been concerns about the specificity of P0-Cre because it is not clear about the timing and spatial distribution of the P0-Cre transgene in labeling NC cells at early embryonic stages. We re-visited P0-Cre and Wnt1-Cre models in the labeling of NC cells in early mouse embryos with a focus on cranial NC. We found that R26-lacZ Cre reporter responded to Cre activity more reliably than CAAG-lacZ Cre reporter during early embryogenesis. Cre immunosignals in P0-Cre and reporter (lacZ and RFP) activity in P0-Cre/R26-lacZ and P0-Cre/R26-RFP embryos were detected in the cranial NC and notochord regions in E8.0–9.5 (4–19 somite) embryos. P0-Cre transgene expression was observed in migrating NC cells and was more extensive in the forebrain and hindbrain but not apparent in the midbrain. Differences in the Cre distribution patterns of P0-Cre and Wnt1-Cre were profound in the midbrain and hindbrain regions, i.e., extensive in the midbrain of Wnt1-Cre and in the hindbrain of P0-Cre embryos. The difference between P0-Cre and Wnt1-Cre in labeling cranial NC may provide a better explanation of the differential distributions of their NC derivatives and of the phenotypes caused by Cre-driven genetic modifications.

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Section: Discussionmentioning

confidence: 99%

Specific and spatial labeling of P0‐Cre versus Wnt1‐Cre in cranial neural crest in early mouse embryos

Chen

Ishan

Yang

et al. 2017

Genesis

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“…Neural crest ectomesenchyme stem cells consist of dental-derived MSCs (DMSCs) and non-dental-derived MSCs (NDMSCs), both of which possess the capacity for dental and periodontal regeneration under certain conditions 16 . Subsets of neural crest-derived cells (NCDCs) also remain as stem cells into adulthood 17 . NCDCs in adults may be used as a stem cell source for tissue regeneration.…”

Section: Tooth Development and Dental Stem Cellsmentioning

confidence: 99%

Wnt and BMP signaling crosstalk in regulating dental stem cells: Implications in dental tissue engineering

et al. 2016

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Tooth is a complex hard tissue organ and consists of multiple cell types that are regulated by important signaling pathways such as Wnt and BMP signaling. Serious injuries and/or loss of tooth or periodontal tissues may significantly impact aesthetic appearance, essential oral functions and the quality of life. Regenerative dentistry holds great promise in treating oral/dental disorders. The past decade has witnessed a rapid expansion of our understanding of the biological features of dental stem cells, along with the signaling mechanisms governing stem cell self-renewal and differentiation. In this review, we first summarize the biological characteristics of seven types of dental stem cells, including dental pulp stem cells, stem cells from apical papilla, stem cells from human exfoliated deciduous teeth, dental follicle precursor cells, periodontal ligament stem cells, alveolar bone-derived mesenchymal stem cells (MSCs), and MSCs from gingiva. We then focus on how these stem cells are regulated by bone morphogenetic protein (BMP) and/or Wnt signaling by examining the interplays between these pathways. Lastly, we analyze the current status of dental tissue engineering strategies that utilize oral/dental stem cells by harnessing the interplays between BMP and Wnt pathways. We also highlight the challenges that must be addressed before the dental stem cells may reach any clinical applications. Thus, we can expect to witness significant progresses to be made in regenerative dentistry in the coming decade.

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“…Furthermore, mineralization was observed in osteoblastic differentiated GFP-positive cell cultures. Lipid droplets were also noted in adipogenic-stimulated cultures utilizing Oil Red O staining, suggesting that oral NCDCs are multipotent and capable of generating osteoblasts and adipocytes 23 . Consistent with our results, Davies et al suggested that NC-derived progenitors in oral mucosa possess a multipotent capacity 11 .…”

Section: Clinical Potential Of Adult Neural Crest-derived Cellsmentioning

confidence: 98%

“…In our study, NCDCs were isolated from the lamina propria of the buccal mucosa, and then cultured in sphere-forming and osteoblastic differentiation media. Following isolation of GFP-positive and -negative cells using flow cytometry with a cell sorter, 10 -15 of the buccal mucosa lamina propria cells were found to be positive for GFP 23 , When isolated from the lamina propria of the buccal mucosa and cultured, GFP-positive cells formed spheres, a stem cell phenotype, and produced alkaline phosphatase ALP , a marker enzyme of the early stage of osteoblastic differentiation Fig. 3 .…”

Section: Clinical Potential Of Adult Neural Crest-derived Cellsmentioning

confidence: 99%

Neural Crest-derived Cells in the Oral and Maxillofacial Regions of Adult Mice: Isolation and Application for Regenerative Medicine

Suzawa

Yoshida

Itose

et al. 2016

Showa Univ J Med Sci

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: Neural crest cells emerge from the dorsal region of the fusing neural tube in vertebrate embryos, then migrate throughout tissues to differentiate into various cell types, including osteoblasts. In adults, subsets of neural crest-derived cells NCDCs reside as stem cells and are considered to be useful cell sources for regenerative medicine. Previous studies have suggested that these NCDC subsets persist into adulthood in mammals, especially those cells within the craniofacial compartments. Recently, our group found that NCDCs were scattered throughout tissues of the palate, gingiva, tongue, hair follicle, submandibular glands, and buccal mucosa of adult mice. NCDCs from the buccal mucosa can also form neurosphere-like structures that have the capability to differentiate into osteoblasts in the presence of bone morphogenetic protein-2. In addition, NCDCs in adults have characteristic gene expression pro les, especially their cell surface molecules. Thus, cell sorting using several speci c cell surface molecules has been proposed as a useful method for isolating NCDCs with high purity. Together, these results suggest that NCDCs reside in various adult oral and maxillofacial regions, and possess the potential to differentiate into osteoblastic cells, indicating that these cells in adults may be a useful source for bone regeneration strategies. In this review, we discuss the distribution, isolation and osteoblastic differentiation potential of NCDCs isolated from various adult tissue sources in the oral and maxillofacial regions.

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Localization and osteoblastic differentiation potential of neural crest-derived cells in oral tissues of adult mice

Cited by 13 publications

References 31 publications

Specific and spatial labeling of P0‐Cre versus Wnt1‐Cre in cranial neural crest in early mouse embryos

Specific and spatial labeling of P0‐Cre versus Wnt1‐Cre in cranial neural crest in early mouse embryos

Wnt and BMP signaling crosstalk in regulating dental stem cells: Implications in dental tissue engineering

Neural Crest-derived Cells in the Oral and Maxillofacial Regions of Adult Mice: Isolation and Application for Regenerative Medicine

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