Localization-based full-field microscopy: how to attain super-resolved images

Son, Taehwang; Lee, Won-Ju; Kim, Donghyun

doi:10.1038/srep12365

Cited by 13 publications

(7 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1d ). We note that the high resonance amplitude obtained with this mode should translate into high contrast imaging and thus improves the resolution of imaging 41 . Furthermore, the fact that the optical field is strongly confined in the holes and less distributed on the surface improves the spatial resolution, which suggests the suitability of the TE mode for imaging.…”

Section: Resultsmentioning

confidence: 91%

Dielectric nanohole array metasurface for high-resolution near-field sensing and imaging

et al. 2021

View full text Add to dashboard Cite

Dielectric metasurfaces support resonances that are widely explored both for far-field wavefront shaping and for near-field sensing and imaging. Their design explores the interplay between localised and extended resonances, with a typical trade-off between Q-factor and light localisation; high Q-factors are desirable for refractive index sensing while localisation is desirable for imaging resolution. Here, we show that a dielectric metasurface consisting of a nanohole array in amorphous silicon provides a favourable trade-off between these requirements. We have designed and realised the metasurface to support two optical modes both with sharp Fano resonances that exhibit relatively high Q-factors and strong spatial confinement, thereby concurrently optimizing the device for both imaging and biochemical sensing. For the sensing application, we demonstrate a limit of detection (LOD) as low as 1 pg/ml for Immunoglobulin G (IgG); for resonant imaging, we demonstrate a spatial resolution below 1 µm and clearly resolve individual E. coli bacteria. The combined low LOD and high spatial resolution opens new opportunities for extending cellular studies into the realm of microbiology, e.g. for studying antimicrobial susceptibility.

show abstract

Section: Resultsmentioning

confidence: 91%

Dielectric nanohole array metasurface for high-resolution near-field sensing and imaging

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The secondary mode appears in part to be contributed by the inter-aperture coupling of localized SP, thereby becoming more prominent when aperture length increases compared to the aperture period. One way to circumvent secondary localization is to employ a long period between apertures, although this may deteriorate full-field imaging resolution 68 . Use of a short aperture length may reduce the strength of the secondary modes: if an aperture is too small, the main and the secondary modes may be created together in the diffraction-limited field-of-view, because the location of the secondary modes is largely defined by the aperture structure.…”

Section: Resultsmentioning

confidence: 99%

“…The nanopost apertures were modeled of gold with periods ranging from Λ = 300 to 900 nm in a step of 200 nm. The range of periods precludes near-field inter-aperture coupling as well as degradation of resolution in potential imaging applications 68 . Gold nanoposts were considered with 15 nm height in each of the aperture models on a 30-nm thick ITO layer and a quartz substrate, as shown in Fig.…”

Section: Model and Methodsmentioning

confidence: 99%

Sub-10 nm near-field localization by plasmonic metal nanoaperture arrays with ultrashort light pulses

Lee

Kim

2015

Sci Rep

Self Cite

View full text Add to dashboard Cite

Near-field localization by ultrashort femtosecond light pulses has been investigated using simple geometrical nanoapertures. The apertures employ circular, rhombic, and triangular shapes to localize the distribution of surface plasmon. To understand the geometrical effect on the localization, aperture length and period of the nanoapertures were varied. Aperture length was shown to affect the performance more than aperture period due mainly to intra-aperture coupling of near-fields. Triangular apertures provided the strongest spatial localization below 10 nm in size as well as the highest enhancement of field intensity by more than 7000 times compared to the incident light pulse. Use of ultrashort pulses was found to allow much stronger light localization than with continuous-wave light. The results can be used for super-localization sensing and imaging applications where spatially localized fields can break through the limits in achieving improved sensitivity and resolution.

show abstract

“…1d). We note that the high dynamic range obtained with this mode should translate into high contrast imaging and thus improves the resolution imaging [41]. Furthermore, the fact that the optical eld is strongly con ned in the holes and less distributed on the surface improves the spatial resolution, which suggests the suitability of the TE mode for imaging.…”

Section: Single Bacteria Detection With Resonant Hyperspectral Imagingmentioning

confidence: 86%

Dielectric Nanohole Array Metasurface For High-Resolution Near-Field Sensing and Imaging

Conteduca

Barth

Pitruzzello

et al. 2020

Preprint

View full text Add to dashboard Cite

Dielectric metasurfaces support resonances that are widely explored both for far-field wavefront shaping and for near-field sensing and imaging. Their design explores the interplay between localised Mie resonances and extended Bragg resonances, with a typical trade-off between Q-factor and light localisation; high Q-factors are desirable for refractive index sensing while localisation is desirable for imaging resolution. Here, we show that a dielectric metasurface consisting of a nanohole array in amorphous silicon provides a favourable trade-off between these requirements. We have designed and realised the metasurface to support two optical modes both with sharp Fano resonances that exhibit relatively high Q factors and strong spatial confinement, thereby concurrently optimizing the device for both imaging and biochemical sensing. For the sensing application, we demonstrate a limit of detection (LOD) as low as 1pg/ml for Immunoglobulin G (IgG); for resonant imaging, we demonstrate a spatial resolution below 1µm and clearly resolve individual E.coli bacteria. The combined low LOD and high spatial resolution opens new opportunities for extending cellular studies into the realm of microbiology, e.g. for studying antimicrobial susceptibility.

show abstract

Localization-based full-field microscopy: how to attain super-resolved images

Cited by 13 publications

References 39 publications

Dielectric nanohole array metasurface for high-resolution near-field sensing and imaging

Dielectric nanohole array metasurface for high-resolution near-field sensing and imaging

Sub-10 nm near-field localization by plasmonic metal nanoaperture arrays with ultrashort light pulses

Dielectric Nanohole Array Metasurface For High-Resolution Near-Field Sensing and Imaging

Contact Info

Product

Resources

About