Localization in Human Term Placental Bed and Amniochorion of Cells Bearing Trophoblast Antigens Identified by Monoclonal Antibodies*

Johnson, Peter; Molloy, C.M.

doi:10.1111/j.1600-0897.1983.tb00250.x

Cited by 31 publications

(6 citation statements)

References 11 publications

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“…The murine mAb 18–6, which recognises an acrosome‐associated antigen 32 was kindly provided by Professor Harry Moore (University of Sheffield, UK). An unrelated mAb prepared in‐house, H317, was also used as a negative control 33 . Texas Red (TR)‐conjugated antimouse IgG was obtained from Molecular Probes (Europe).…”

Section: Methodsmentioning

confidence: 99%

The complement regulatory proteins CD55 (decay accelerating factor) and CD59 are expressed on the inner acrosomal membrane of human spermatozoa as well as CD46 (membrane cofactor protein)

et al. 2006

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Summary The complement regulatory proteins CD55 and CD59 are expressed on the plasma membrane of human spermatozoa, whereas CD46 is only on the inner acrosomal membrane (IAM) which becomes surfaced exposed after the acrosome reaction when sperm assume fertilisation‐competence. CD55 & CD59, two glycosylphosphatidylinositol (GPI)‐anchored proteins, have been detected previously in some studies also in the acrosomal region of chemically fixed spermatozoa but never demonstrated at this site on unfixed spermatozoa. Dual labelling immunofluorescence and confocal microscopy on fresh unfixed spermatozoa, with minimal subsequent time to fixation, has shown CD55 to be markedly expressed on the IAM, more than on the plasma membrane. However, unlike for CD46, CD55 displayed patchy staining over the acrosome, with some variation between individual spermatozoa. All IAM‐associated CD55 was localised within GM1‐containing lipid rafts. CD59 was expressed also on the IAM, but in a pronounced granular pattern with more variation observed from one spermatozoa to another. Both CD55 & CD59 were released from the IAM by PI‐PLC, demonstrating them to be GPI‐anchored. Analysis of acrosome‐reacted spermatozoal CD55 by Western blotting revealed a novel single 55 kDa protein lacking significant oligosaccharides susceptible to glycosidases. Antibody‐induced membrane rafting and release of CD55 & CD59 in vitro may have influenced previous results. Significant coexpression of CD55 & CD46 on the IAM suggests some functional cooperation at this site.

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Section: Methodsmentioning

confidence: 99%

The complement regulatory proteins CD55 (decay accelerating factor) and CD59 are expressed on the inner acrosomal membrane of human spermatozoa as well as CD46 (membrane cofactor protein)

et al. 2006

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“…The H317 hybridoma had been produced after immunization with isolated plasma membrane preparations from syncytiotrophoblast microvilli of normal human term placentae (Johnson et al, 1981;Johnson & Molloy, 1983). This mAb is specific for the heat-stable, L-phenylalanine-inhibitable, PLAP isoenzyme and is unreactive with other AP isoenzymes from normal human tissues (McLaughlin et al, 1982(McLaughlin et al, , 1984a A sensitive solid-phase EIA using the H317 mAb, described in detail previously (McLaughlin et al, , 1984a, was used to determine active PLAP in sera collected prior to laparotomy and in fresh tumour tissue extracts.…”

Section: Monoclonal Antibodymentioning

confidence: 99%

Detection of placental-type alkaline phosphatase in ovarian cancer

McDicken¹,

Pj²,

Tromans³

et al. 1985

Br J Cancer

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Summary A monoclonal antibody, H317, has been used for the sensitive and specific detection of placentaltype alkaline phosphatase (PLAP) in sera, solubilized tissue extracts and fixed tumour tissue sections from patients representing a variety of ovarian tumours. PLAP was detected in over 30% of these sera and in most solubilized tumour tissue extracts. There was no association between circulating PLAP levels and either tissue extract levels or immunohistological staining of ovarian tumour tissue sections with H317. Nevertheless, immunohistology demonstrated the heterogeneity of cellular localization of PLAP within different tumours, and can often be of value in localizing tumour tissue.

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“…They suggested that these antibodies recognize substances similar to pregnancy-associated proteins which could either be produced by glandular tissue and actively taken up by trophoblast or be produced by trophoblast and absorbed by glandular epithelium. The former possibility was dismissed by the authors as unlikely on the assumption that no glands were present in the placental bed at term, yet trophoblast from term placenta continued to show reactivity to the monoclonal antibodies (Johnson et al, 1981;Johnson and Molloy, 1983). The current study's identification of glands active in glycoprotein synthesis at term again raises the possibility that they could have a role in secreting the antigen recognized by the two monoclonal antibodies.…”

Section: Discussionmentioning

confidence: 65%

Morphology and glycoprotein synthesis of uterine glandular epithelium in human basal plate at term: An ultrastructural and autoradiographic study

Nelson

Ortman-Nabi

1986

Anat. Rec.

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This study describes the morphologic features of uterine glandular epithelium in human basal plate at term and identifies this epithelium as an active site of glycoprotein synthesis. Wedge biopsies were obtained from the basal plate at the time of repeat cesarean section from 11 normal pregnant patients at term. Biopsy specimens were either processed immediately for microscopic examination or incubated in vitro with 25 microCi/cc of 3H-galactose or 3H-leucine. Tissues incubated with tritiated compounds (2-hour pulse +/- 3-hour chase in nonradioactive medium) were either processed for light microscopic autoradiographic analysis or extracted for determination of trichloroacetic-acid-precipitable tritiated macromolecules in tissues and medium. Profiles of cuboidal-columnar glandular epithelium were identified in the decidual component of the basal plate region adjacent to spiral arterioles and perpendicular to the inner layers of myometrial muscle. Autoradiographic and biochemical studies identified the glandular epithelium, as well as large decidual cells, to be major sites of incorporation of both 3H-galactose and 3H-leucine and to be prime sources for secretion of tritiated macromolecules that appeared in the medium during in vitro incubation of basal plate tissue. Ultrastructurally, the glandular epithelium was noted to rest on a basal lamina, to have lateral cell membranes with numerous desmosomes, and to exhibit an apical surface with microvilli projecting into a luminal space. Cytologic features of the cells included abundant profiles of rough endoplasmic reticulum, multiple mitochondria with lamellar cristae, a well-developed perinuclear but nonpolarized Golgi apparatus,and nuclei containing predominantly euchromatin.(ABSTRACT TRUNCATED AT 250 WORDS)

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Localization in Human Term Placental Bed and Amniochorion of Cells Bearing Trophoblast Antigens Identified by Monoclonal Antibodies*

Cited by 31 publications

References 11 publications

The complement regulatory proteins CD55 (decay accelerating factor) and CD59 are expressed on the inner acrosomal membrane of human spermatozoa as well as CD46 (membrane cofactor protein)

The complement regulatory proteins CD55 (decay accelerating factor) and CD59 are expressed on the inner acrosomal membrane of human spermatozoa as well as CD46 (membrane cofactor protein)

Detection of placental-type alkaline phosphatase in ovarian cancer

Morphology and glycoprotein synthesis of uterine glandular epithelium in human basal plate at term: An ultrastructural and autoradiographic study

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