Localization of 5‐hydroxytryptamine‐like immunoreactive cells and nerve fibers in the rat female reproductive system

Amenta, Francesco; Ja, Vega; Rícci, Alberto; Collier, Wade L.

doi:10.1002/ar.1092330315

Cited by 51 publications

(29 citation statements)

References 23 publications

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“…Taken together, the current findings support an important role of 5-HT on the migration of sperm throughout the female tract. The possibility that 5-HT plays a role in sperm physiology may occur at 5-HT receptors and/or 5-HT transporters located on the surface of sperm, responding to the 5-HT contained in the uterine or seminal fluid (Mann et al 1961, Amenta et al 1992. However, the surprising finding that some other serotoninergic elements are intracellular remains unclear and requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Evidence of 5-HT components in human sperm: implications for protein tyrosine phosphorylation and the physiology of motility

Jiménez-Trejo¹,

Tapia-Rodríguez²,

Cerbón³

et al. 2012

Reproduction

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Serotonin (5-hydroxytryptamine; C 10 H 12 N 2 O (5-HT)) is produced in the CNS and in some cells of peripheral tissues. In the mammalian male reproductive system, both 5-HT and tryptophan hydroxylase (TPH) have been described in Leydig cells of the testis and in principal cells of the caput epididymis. In capacitated hamster sperm, it has been shown that 5-HT promotes the acrosomal reaction. The aim of this work was to explore the existence of components of the serotoninergic system and their relevance in human sperm physiology. We used both immunocytochemistry and western blot to detect serotoninergic markers such as 5-HT, TPH1, MAO A , 5-HT 1B , 5-HT 3 , and 5HT T ; HPLC for TPH enzymatic activity; Computer Assisted Semen Analysis assays to measure sperm motility parameters and pharmacological approaches to show the effect of 5-HT in sperm motility and tyrosine phosphorylation was assessed by western blot. We found the presence of serotoninergic markers (5-HT, TPH1, MAO A , 5-HT 1B , 5-HT 2A , 5-HT 3 , 5-HT T , and TPH enzymatic activity) in human sperm. In addition, we observed a significant increase in tyrosine phosphorylation and changes in sperm motility after 5-HT treatment. In conclusion, our data demonstrate the existence of components of a serotoninergic system in human sperm and support the notion for a functional role of 5-HT in mammalian sperm physiology, which can be modulated pharmacologically.

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Section: Discussionmentioning

confidence: 99%

Evidence of 5-HT components in human sperm: implications for protein tyrosine phosphorylation and the physiology of motility

Jiménez-Trejo¹,

Tapia-Rodríguez²,

Cerbón³

et al. 2012

Reproduction

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“…This PCR-based assay could also detect a minimum of 10 femtograms. These results indicate that serotonin present in embryos could arise either through synthesis by TPH2 or by transport from extracellular sources, for example, serotonin present in the tubal fluid of the oviduct (Juorio et al 1989, Amenta et al 1992, Yavarone et al 1993. The multiplicity of the mechanisms for obtaining serotonin may indicate the importance of this molecule to the embryo.…”

Section: Serotonin In Mitochondria In Mouse Embryosmentioning

confidence: 93%

“…In mammals, such studies have been lacking, though in mice, drastic effects are observed in the early embryo upon blocking tryptophan hydroxylase, the rate-limiting enzyme in serotonin synthesis. Serotonin is present in the tubal fluid of the oviduct, cervix, and uterus (Juorio et al 1989, Amenta et al 1992, Yavarone et al 1993). On administering p-chlorophenylalanine, a tryptophan hydroxylase inhibitor, to pregnant mice, it was observed that the cleavage divisions of the zygote were arrested and in cases where the embryo proceeded to the blastocyst stage, it was defective and failed to implant (Khozhai et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Serotonin in pre-implantation mouse embryos is localized to the mitochondria and can modulate mitochondrial potential

Basu¹,

Desai²,

Balaji³

et al. 2008

Reproduction

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Serotonin is reported to be present in early embryos of many species and plays an important role in early patterning. Since it is a fluorophore, it can be directly visualized using fluorescence microscopy. Here, we use three-photon microscopy to image serotonin in live pre-implantation mouse embryos. We find that it is present as puncta averaging 1.3 square microns and in concentrations as high as 442 mM. The observed serotonin puncta were found to co-localize with mitochondria. Live embryos pre-incubated with serotonin showed a higher mitochondrial potential, indicating that it can modulate mitochondrial potential. Pre-implantation mouse embryos were also examined at various developmental stages for the presence of transcripts of the peripheral and neuronal forms of tryptophan hydroxylase (Tph1 and Tph2 respectively) and the classical serotonin transporter (Slc6a4). Transcripts of Tph2 were seen in oocytes and in two-cell stages, whereas transcripts of Tph1 were not detected at any stage. Transcripts of the transporter, Slc6a4, were present in all pre-implantation stages investigated. These results suggest that serotonin in embryos can arise from a combination of synthesis and uptake from the surrounding milieu.

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“…Among the indications that 5-HT might be such a local regulator is its detection in female rodent genital tracts (Amenta et al, 1992) and in human follicular fluid (Bodis et al, 1993). Moreover, 5-HT has also been reported recently in isolated mouse oocytes and embryos (Il'kova et al, 2004;Amireault and Dubé, 2005) and in surrounding cumulus cells that also possess the rate-limiting enzyme tryptophan hydroxylase for 5-HT production, thus making these cells a potential immediate direct source of 5-HT (Amireault and Dubé, 2005).…”

mentioning

confidence: 97%

Intracellular cAMP and Calcium Signaling by Serotonin in Mouse Cumulus-Oocyte Complexes

Amireault

Dubé²

2005

Mol Pharmacol

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cAMP and intracellular Ca 2ϩ are important second messengers involved in mammalian follicular growth and oocyte meiotic maturation. We investigated the capacity of the neurohormone serotonin (5-hydroxytryptamine, 5-HT) to regulate intracellular cAMP and Ca 2ϩ in mouse oocytes and surrounding cumulus cells. On the basis of a reverse transcription-polymerase chain reaction study, 5-HT 7 receptor mRNA is expressed in cumulus cells, oocytes, and embryos up to the four-cell stage, and 5-HT 2A and 5-HT 2B receptor mRNAs are expressed in cumulus cells only, whereas 5-HT 2C , 5-HT 4 , and 5-HT 6 receptors are expressed in neither oocytes nor cumulus cells. The addition of 5-HT (10 nM to 10 M) to isolated metaphase II oocytes had no effect on their internal cAMP or Ca 2ϩ levels, whereas it caused dose-dependent cAMP and Ca 2ϩ increases in cumulus cells. This cAMP increase in cumulus cells could be mimicked by 5-HT agonists with the following order of potency: 5-HT Ͼ 8-hydroxy-2-(di-n-propylamino) tetralin ϭ ␣-methyl-5-HT ϭ 5-carboxamidotryptamine maleate Ͼ 2-[1-(4-piperonyl)piperazinyl]benzo-triazole, thereby supporting a preferential involvement of 5-HT 7 receptors. As measured with cumulus cells preloaded with fura-2/acetoxymethyl ester (AM), the addition of 5-HT also caused dose-dependent Ca 2ϩ increases, which were probably linked to detected 5-HT 2A and 5-HT 2B receptors.

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Localization of 5‐hydroxytryptamine‐like immunoreactive cells and nerve fibers in the rat female reproductive system

Cited by 51 publications

References 23 publications

Evidence of 5-HT components in human sperm: implications for protein tyrosine phosphorylation and the physiology of motility

Evidence of 5-HT components in human sperm: implications for protein tyrosine phosphorylation and the physiology of motility

Serotonin in pre-implantation mouse embryos is localized to the mitochondria and can modulate mitochondrial potential

Intracellular cAMP and Calcium Signaling by Serotonin in Mouse Cumulus-Oocyte Complexes

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