Localization of cortical tissue optical changes during seizure activity in vivo with optical coherence tomography

Eberle, Melissa; Hsu, Mike S.; Rodriguez, Carissa L.; Szu, Jenny I.; Oliveira, Michael; Binder, Devin K.; Park, B. Hyle

doi:10.1364/boe.6.001812

Cited by 9 publications

(5 citation statements)

References 37 publications

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“…The drug 4-aminopyridine (4-AP) was used to induce epileptic seizure in various models of epileptic seizure 44 – 48 . Epileptiform activity has been extensively studied using different optical imaging methods 49 – 56 . Among these, photoacoustic tomography has the potential to record deep brain voltage response signals and hemodynamic signals in parallel and to become a new tool for understanding the induction, maintenance, and propagation of seizure discharges.…”

Section: Resultsmentioning

confidence: 99%

Photoacoustic imaging of voltage responses beyond the optical diffusion limit

Rao

Zhang

et al. 2017

Sci Rep

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Non-invasive optical imaging of neuronal voltage response signals in live brains is constrained in depth by the optical diffusion limit, which is due primarily to optical scattering by brain tissues. Although photoacoustic tomography breaks this limit by exciting the targets with diffused photons and detecting the resulting acoustic responses, it has not been demonstrated as a modality for imaging voltage responses. In this communication, we report the first demonstration of photoacoustic voltage response imaging in both in vitro HEK-293 cell cultures and in vivo mouse brain surfaces. Using spectroscopic photoacoustic tomography at isosbestic wavelengths, we can separate voltage response signals and hemodynamic signals on live brain surfaces. By imaging HEK-293 cell clusters through 4.5 mm thick ex vivo rat brain tissue, we demonstrate photoacoustic tomography of cell membrane voltage responses beyond the optical diffusion limit. Although the current voltage dye does not immediately allow in vivo deep brain voltage response imaging, we believe our method opens up a feasible technical path for deep brain studies in the future.

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Section: Resultsmentioning

confidence: 99%

Photoacoustic imaging of voltage responses beyond the optical diffusion limit

Rao

Zhang

et al. 2017

Sci Rep

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“…Moreover, the ability to noninvasively map the variations of CBF over a long period of time using OCT angiography would contribute to the understanding of the complex mechanisms related to TBI recovery [ 3 ]. Some research groups are utilizing OCT to detect cortical optical changes during seizure activity [ 92 , 93 ]. As it is a relatively newer field of research, future studies should compare the temporal performance of seizure detection with OCT with that of gold standard EEG.…”

Section: Oct In Neurologymentioning

confidence: 99%

Optical Coherence Tomography: Basic Concepts and Applications in Neuroscience Research

Mokbul¹

2017

Journal of Medical Engineering

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Optical coherence tomography is a micrometer-scale imaging modality that permits label-free, cross-sectional imaging of biological tissue microstructure using tissue backscattering properties. After its invention in the 1990s, OCT is now being widely used in several branches of neuroscience as well as other fields of biomedical science. This review study reports an overview of OCT's applications in several branches or subbranches of neuroscience such as neuroimaging, neurology, neurosurgery, neuropathology, and neuroembryology. This study has briefly summarized the recent applications of OCT in neuroscience research, including a comparison, and provides a discussion of the remaining challenges and opportunities in addition to future directions. The chief aim of the review study is to draw the attention of a broad neuroscience community in order to maximize the applications of OCT in other branches of neuroscience too, and the study may also serve as a benchmark for future OCT-based neuroscience research. Despite some limitations, OCT proves to be a useful imaging tool in both basic and clinical neuroscience research.

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“…Thus, fOCT is a potentially attractive tool for detecting electrical activity in neural tissue in vivo. [18][19][20][21][22][23][24][25][26][27] For many studies (e.g., aimed at neuromodulation or functional tract tracing), neural stimulation methods are desirable. Electrical stimulation has been a long-standing tool for neurostimulation, 28,29 but it suffers from spread of electrical current, often leading to unwanted activation of additional brain circuits and resulting side effects.…”

Section: Introductionmentioning

confidence: 99%

INS-fOCT: a label-free, all-optical method for simultaneously manipulating and mapping brain function

et al. 2020

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Significance: Current approaches to stimulating and recording from the brain have combined electrical or optogenetic stimulation with recording approaches, such as two-photon, electrophysiology (EP), and optical intrinsic signal imaging (OISI). However, we lack a label-free, all-optical approach with high spatial and temporal resolution. Aim: To develop a label-free, all-optical method that simultaneously manipulates and images brain function using pulsed near-infrared light (INS) and functional optical coherence tomography (fOCT), respectively. Approach: We built a coregistered INS, fOCT, and OISI system. OISI and EP recordings were employed to validate the fOCT signals. Results: The fOCT signal was reliable and regional, and the area of fOCT signal corresponded with the INS-activated region. The fOCT signal was in synchrony with the INS onset time with a delay of ∼30 ms. The magnitude of fOCT signal exhibited a linear correlation with the INS radiant exposure. The significant correlation between the fOCT signal and INS was further supported by OISI and EP recordings. Conclusions: The proposed fiber-based, all-optical INS-fOCT method allows simultaneous stimulation and mapping without the risk of interchannel cross-talk and the requirement of contrast injection and viral transfection and offers a deep penetration depth and high resolution.

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Localization of cortical tissue optical changes during seizure activity in vivo with optical coherence tomography

Cited by 9 publications

References 37 publications

Photoacoustic imaging of voltage responses beyond the optical diffusion limit

Photoacoustic imaging of voltage responses beyond the optical diffusion limit

Optical Coherence Tomography: Basic Concepts and Applications in Neuroscience Research

INS-fOCT: a label-free, all-optical method for simultaneously manipulating and mapping brain function

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