Localization of polysaccharides, proteins and lipids in human and pig blood platelets

Morgenstern, Eberhard; Weber, E.

doi:10.1007/bf00490275

Cited by 7 publications

(1 citation statement)

References 38 publications

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“…Data from the literature indicate that the granule nucleoid contains a large amount of proteoglycan (41,42). The differential localization in the granule may therefore be explained by assuming that the larger fibrinogen molecule (mol wt 340,000) is excluded from the dense proteoglycan accumulation, whereas the much smaller beta thromboglobulin molecule (mol wt 36,000) may penetrate into this area.…”

Section: Resultsmentioning

confidence: 99%

Immunocytochemical localization of fibrinogen, platelet factor 4, and beta thromboglobulin in thin frozen sections of human blood platelets.

Sander¹,

Slot²,

Bouma³

et al. 1983

J. Clin. Invest.

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A B S T R A C T Affinity-purified monospecific antibodies against human fibrinogen and the platelet-specific proteins platelet factor 4 and beta thromboglobulin were used to localize these antigens in thin and ultrathin frozen sections of mildly fixed, washed human blood platelets. By immunofluorescent double-labeling experiments the distribution of fibrinogen was compared to that of platelet factor 4 and beta thromboglobulin. All three antigens occurred in virtually all platelets and showed an identical, dotlike distribution. For immunoelectron microscopy we used protein Acolloidal gold on ultrathin frozen sections to visualize the specific reaction indirectly. The staining for platelet factor 4, beta thromboglobulin, and fibrinogen localized exclusively over a-granules of washed platelets. Within the granules, platelet factor 4 was localized preferentially in the electron dense, a-granule nucleoid, whereas fibrinogen was more predominant in the electron-lucent granule periphery. Beta thromboglobulin localization did not show a preferential intragranular distribution.

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Section: Resultsmentioning

confidence: 99%

Immunocytochemical localization of fibrinogen, platelet factor 4, and beta thromboglobulin in thin frozen sections of human blood platelets.

Sander¹,

Slot²,

Bouma³

et al. 1983

J. Clin. Invest.

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An ultrastructural study of the leukocytes of the channel catfish, Ictalurus punctatus

Cannon¹,

Mollenhauer²,

Eurell³

et al. 1980

Journal of Morphology

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Ultrastructural studies on blood leukocytes of the channel catfish, Ictalurus punctatus, show the presence of heterophils (neutrophils), small lymphocytes, monocytes, and thrombocytes. Monocytes cannot always be distinguished from large lymphocytes. Cells resembling macrophages or transitional forms between monocytes and macrophages are occasionally seen. Blood eosinophils and basophils are not found. Thrombocytes and small lymphocytes are the most abundant leukocytes, while monocytes are the least frequently encountered leukocyte. Glycogen, present in all leukocytes, is most abundant in heterophils and least abundant in monocytes. Although monocytes are similar to heterophils in size and shape, a greater amount of rough endoplasmic reticulum, free ribosomes, and fewer granules are observed in monocytes. Heterophils possess oval or elongate granules, which often contain a crystalline or striated structure; small tubules which resemble smooth endoplasmic reticulum, and cristae which traverse the long axes of the mitochondria are frequently seen. Small lymphocytes are characterized by the presence of pseudopodia, many free ribosomes, numerous large mitochondria, dictyosomes (Golgi), and long profiles of rough endoplasmic reticulum. The dictyosomes are often associated with a large zone of exclusion. Bundles of microtubules are observed near the elongated ends of thrombocytes. Deep indentations of the plasmalemma, which give the appearance of vacuoles, are also seen in thrombocytes.

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Feinstruktur der Thrombozytopoese und ihre Störungen

Morgenstern¹

1976

Elektronenmikroskopische Hämatologie

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Localization of polysaccharides, proteins and lipids in human and pig blood platelets

Cited by 7 publications

References 38 publications

Immunocytochemical localization of fibrinogen, platelet factor 4, and beta thromboglobulin in thin frozen sections of human blood platelets.

Immunocytochemical localization of fibrinogen, platelet factor 4, and beta thromboglobulin in thin frozen sections of human blood platelets.

An ultrastructural study of the leukocytes of the channel catfish, Ictalurus punctatus

Feinstruktur der Thrombozytopoese und ihre Störungen

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