Localization of prodynorphin messenger RNA in rat brain by in situ hybridization using a synthetic oligonucleotide probe

Morris, Brian J.; Haarmann, I.; Kempter, B.; Höllt, Volker; Herz, A.

doi:10.1016/0304-3940(86)90423-4

Cited by 87 publications

(24 citation statements)

References 16 publications

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“…The arcuate nucleus of the hypothalamus is a key area in regulating food intake and energy homeostasis, where both NPY and prodynorphin mRNA are reported to be expressed. This further supports a major role for these two peptides in modulating of energy balance , Morris et al, 1986and Sato et al, 1991.…”

Section: Introductionsupporting

confidence: 72%

“…Prodynorphin mRNA expression has also been investigated in the rat brain, showing high levels of expression in the hippocampus, striatum, amygdala and hypothalamus (Morris et al, 1986, Sato et al, 1991and Merchenthaler et al, 1997. However, there are no studies so far describing the distribution and regulation of prodynorphin mRNA in the mouse brain.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of prodynorphin mRNA and its interaction with the NPY system in the mouse brain

et al. 2006

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Using radioactive in situ hybridisation, the distribution of prodynorphin mRNA in the brains of C57Bl/6 mice was systemically investigated, and double-labelling in situ hybridisation was used to determine the extent to which neuropeptide Y (NPY) and prodynorphin mRNAs were co-expressed. Our results demonstrate that prodynorphin mRNA expression in the mouse brain is localised at specific subregions of the olfactory bulb, cortex, hippocampus, amygdala, basal ganglia, thalamus, hypothalamus, mesencephalon and myelencephalon. Among the regions displaying the most intense labelling were the olfactory tubercle, lateral septum (LS), caudate putamen (Cpu), central amygdaloid nucleus (Ce), paraventricular hypothalamic nucleus (PVN), supraoptic nucleus (SO), lateral hypothalamic area (LHA), ventromedial hypothalamic nucleus (VMH), lateral reticular nucleus (LRt) and solitary tract nucleus (NTS). In the arcuate nucleus of the hypothalamus (Arc), doublelabelling in situ hybridisation revealed that prodynorphin expressing neurons also contained NPY mRNA, with a co-localisation rate of approximately 88% in the lateral part of the Arc, and 79% in the dorsal part of the Arc, respectively, suggesting potential overlapping functions of these two neurotransmitters in feeding type behaviour.

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Section: Introductionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Distribution of prodynorphin mRNA and its interaction with the NPY system in the mouse brain

et al. 2006

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“…The regional distribution of the hybridization with each probe parallels the regional distribution of immunoreactive neurons noted in immunohistochemical studies (e.g., Vincent et al, 1982;Williams and Dockray, 1983) and of the distribution of these mRNAs noted by other laboratories (Bloch et al, 1986;Morris et al, 1986;Siegel and Young, 1986;Shivers et al, 1986;Harlan et al, 1987;Lightman and Young, 1987). Thus, there are hybridization densities over the striatum for each probe but hybridization over the supraoptic nuclei only with the dynorphin probes (Fig.…”

Section: Resultsmentioning

confidence: 76%

Differential expression of preproenkephalin and preprodynorphin mRNAs in striatal neurons: high levels of preproenkephalin expression depend on cerebral cortical afferents

1988

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Preproenkephalin and preprodynorphin mRNAs can be detected by in situ hybridization in medium-sized striatal neurons in normal rats and in rats with unilateral cerebral cortical lesions. Hybridization of 35S- labeled oligonucleotide cDNAs complementary to specific regions of each mRNA reveals that preproenkephalin-expressing neurons are more numerous than cells expressing preprodynorphin. Hybridization densities above enkephalin-positive neurons are also more than twice those noted above preprodynorphin-expressing cells. Northern analyses of mRNA extracted from the striatum are consistent with these relationships. The striatal preproenkephalin hybridization densities are decreased ipsilateral to cerebral cortical lesions; this change evolves largely between 1 and 5 d following the lesion. Striatal preproenkephalin mRNA is thus more prominent than preprodynorphin mRNA and depends on cerebral cortical inputs for its full expression.

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“…Moderate to high levels of dynorphin mRNA and κ opioid receptors are expressed in regions of the brain that are stress-related in rodents, including the hypothalamic paraventricular nucleus (PVN), amygdala (AMY), hippocampus (Hip) and bed nucleus of the stria terminalis (BNST) [11,26,27] , and stress exposure has been shown to increase endogenous dynorphin levels [28] . A growing body of evidence reveals that the dynorphin/κ opioid receptor system plays an important role in stress [29][30][31] .…”

Section: Rodent Models Of Anxietymentioning

confidence: 99%

The role of the dynorphin/κ opioid receptor system in anxiety

Hang

Wang

et al. 2015

Acta Pharmacol Sin

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Anxiety disorders are the most common and prevalent forms of psychiatric disease, although the biological basis of anxiety is not well understood. The dynorphin/κ opioid receptor system is widely distributed in the central nervous system and has been shown to play a critical role in modulating mood and emotional behaviors. In the present review, we summarize current literature relating to the role played by the dynorphin/κ opioid receptor system in anxiety and κ opioid receptor antagonists as potential therapeutic agents for the treatment of anxiety disorders.

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Localization of prodynorphin messenger RNA in rat brain by in situ hybridization using a synthetic oligonucleotide probe

Cited by 87 publications

References 16 publications

Distribution of prodynorphin mRNA and its interaction with the NPY system in the mouse brain

Distribution of prodynorphin mRNA and its interaction with the NPY system in the mouse brain

Differential expression of preproenkephalin and preprodynorphin mRNAs in striatal neurons: high levels of preproenkephalin expression depend on cerebral cortical afferents

The role of the dynorphin/κ opioid receptor system in anxiety

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