Locus-specific proteome decoding reveals Fpt1 as a chromatin-associated negative regulator of RNA polymerase III assembly

van Breugel, Maria Elize; van Kruijsbergen, Ila; Mittal, Chitvan; Lieftink, Cor; Brouwer, Ineke; van den Brand, Teun; Kluin, Roelof J.C.; Hoekman, Liesbeth; Menezes, Renée X.; van Welsem, Tibor; Del Cortona, Andrea; Malik, Muddassir; Beijersbergen, Roderick L.; Lenstra, Tineke L.; Verstrepen, Kevin J.; Pugh, B. Franklin; van Leeuwen, Fred

doi:10.1016/j.molcel.2023.10.037

Cited by 2 publications

(1 citation statement)

References 86 publications

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“…Alternatively, specific DNA-binding proteins can also regulate the binding of the general RNA Pol III transcription factors TFIIIB and TFIIIC to individual tRNA genes similar to the mechanism described in mammalian cells [52]. Indeed, the regulator of yeast RNA Pol III assembly Fpt1 binds to promoters of some tRNA genes and modulates their transcription [53].…”

Section: Abundance Of Trna-synthesis and Degradationmentioning

confidence: 95%

Non-Coding RNAs: Regulators of Stress, Ageing, and Developmental Decisions in Yeast?

Čáp,

Palková

2024

Cells

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Cells must change their properties in order to adapt to a constantly changing environment. Most of the cellular sensing and regulatory mechanisms described so far are based on proteins that serve as sensors, signal transducers, and effectors of signalling pathways, resulting in altered cell physiology. In recent years, however, remarkable examples of the critical role of non-coding RNAs in some of these regulatory pathways have been described in various organisms. In this review, we focus on all classes of non-coding RNAs that play regulatory roles during stress response, starvation, and ageing in different yeast species as well as in structured yeast populations. Such regulation can occur, for example, by modulating the amount and functional state of tRNAs, rRNAs, or snRNAs that are directly involved in the processes of translation and splicing. In addition, long non-coding RNAs and microRNA-like molecules are bona fide regulators of the expression of their target genes. Non-coding RNAs thus represent an additional level of cellular regulation that is gradually being uncovered.

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Section: Abundance Of Trna-synthesis and Degradationmentioning

confidence: 95%

Non-Coding RNAs: Regulators of Stress, Ageing, and Developmental Decisions in Yeast?

Čáp,

Palková

2024

Cells

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The choreography of chromatin in RNA polymerase III regulation

van Breugel,

Gerber,

van Leeuwen

2024

Biochemical Society Transactions

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Regulation of eukaryotic gene expression involves a dynamic interplay between the core transcriptional machinery, transcription factors, and chromatin organization and modification. While this applies to transcription by all RNA polymerase complexes, RNA polymerase III (RNAPIII) seems to be atypical with respect to its mechanisms of regulation. One distinctive feature of most RNAPIII transcribed genes is that they are devoid of nucleosomes, which relates to the high levels of transcription. Moreover, most of the regulatory sequences are not outside but within the transcribed open chromatin regions. Yet, several lines of evidence suggest that chromatin factors affect RNAPIII dynamics and activity and that gene sequence alone does not explain the observed regulation of RNAPIII. Here we discuss the role of chromatin modification and organization of RNAPIII transcribed genes and how they interact with the core transcriptional RNAPIII machinery and regulatory DNA elements in and around the transcribed genes.

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Locus-specific proteome decoding reveals Fpt1 as a chromatin-associated negative regulator of RNA polymerase III assembly

Cited by 2 publications

References 86 publications

Non-Coding RNAs: Regulators of Stress, Ageing, and Developmental Decisions in Yeast?

Non-Coding RNAs: Regulators of Stress, Ageing, and Developmental Decisions in Yeast?

The choreography of chromatin in RNA polymerase III regulation

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