Long-Distance Signaling in Nodulation Directed by a CLAVATA1-Like Receptor Kinase

Searle, Iain; Men, A.; Laniya, T. S.; Buzas, Diana Mihaela; Iturbe‐Ormaetxe, Iñaki; Carroll, Bernard J.; Gresshoff, Peter M.

doi:10.1126/science.1077937

Cited by 495 publications

(416 citation statements)

References 26 publications

Supporting

Mentioning

394

Contrasting

Unclassified

Order By: Relevance

“…This hypothesis was strongly supported by the findings from analyses of the Lotus japonicus hypernodulating mutant, hypernodulation aberrant root formation (har1) [8][9][10] , and orthologous mutants in other leguminous species 8,9,11,12 . HAR1 encodes a leucine-rich repeat receptor kinase (LRR-RK) that is expressed in both roots and shoots 8,9 .…”

supporting

confidence: 64%

Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase

et al. 2013

View full text Add to dashboard Cite

Leguminous plants establish a symbiosis with rhizobia to enable nitrogen fixation in root nodules under the control of the presumed root-to-shoot-to-root negative feedback called autoregulation of nodulation. In Lotus japonicus, autoregulation is mediated by CLE-RS genes that are specifically expressed in the root, and the receptor kinase HAR1 that functions in the shoot. However, the mature functional structures of CLE-RS gene products and the molecular nature of CLE-RS/HAR1 signalling governed by these spatially distant components remain elusive. Here we show that CLE-RS2 is a post-translationally arabinosylated glycopeptide derived from the CLE domain. Chemically synthesized CLE-RS glycopeptides cause significant suppression of nodulation and directly bind to HAR1 in an arabinose-chain and sequencedependent manner. In addition, CLE-RS2 glycopeptide specifically produced in the root is found in xylem sap collected from the shoot. We propose that CLE-RS glycopeptides are the long sought mobile signals responsible for the initial step of autoregulation of nodulation.

show abstract

supporting

confidence: 64%

Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Mutations in En 1314 and En 1282 soybeans were found in the NFR1 gene, which is known to be responsible for a LysM-type receptor kinase for Nod factor recognition (Radutoiu et al, 2003 Masaki Hayashi (National Institute of Agrobiological Sciences, Tsukuba, Japan), personal communication). A mutation in En 6500 was found in the GmNARK (NTS1) gene (Nishimura et al, 2002), which mediates systemic autoregulation of nodulation (Searle et al, 2003), and Sakukei 4 is a descendant with a hypernodulating phenotype that was derived from En 6500 (Matsunami et al, 2004).…”

Section: Plant Materials and Field Experimental Designmentioning

confidence: 99%

Community shifts of soybean stem-associated bacteria responding to different nodulation phenotypes and N levels

et al. 2009

View full text Add to dashboard Cite

The diversity of stem-associated bacteria of non-nodulated (Nod À ), wild-type nodulated (Nod þ ) and hypernodulated (Nod þ þ ) soybeans were evaluated by clone library analyses of the 16S ribosomal RNA gene. Soybeans were dressed with standard nitrogen (SN) fertilization (15 kg N ha -1 ) and heavy nitrogen (HN) fertilization (615 kg N ha -1 ). The relative abundance of Alphaproteobacteria in Nod þ soybeans (66%) was smaller than that in Nod À and Nod þ þ soybeans (75-76%) under SN fertilization, whereas that of Gammaproteobacteria showed the opposite pattern (23% in Nod þ and 12-16% in Nod À and Nod þ þ soybeans). Principal coordinate analysis showed that the bacterial communities of Nod À and Nod þ þ soybeans were more similar to each other than to that of Nod þ soybeans under SN fertilization. HN fertilization increased the relative abundance of Gammaproteobacteria in all nodulation phenotypes (33-57%) and caused drastic shifts of the bacterial community. The clustering analyses identified a subset of operational taxonomic units (OTUs) at the species level in Alpha-and Gammaproteobacteria responding to both the nodulation phenotypes and nitrogen fertilization levels. Meanwhile, the abundance of Betaproteobacteria was relatively constant in all libraries constructed under these environmental conditions. The relative abundances of two OTUs in Alphaproteobacteria (Aurantimonas sp. and Methylobacterium sp.) were especially sensitive to nodulation phenotype and were drastically decreased under HN fertilization. These results suggested that a subpopulation of proteobacteria in soybeans is controlled in a similar manner through both the regulation systems of plant-rhizobia symbiosis and the nitrogen signaling pathway in plants.

show abstract

“…However, there are clearly examples of where comparative mapping and the use of markers from other legume species can aid gene identification in soybean (e.g. Searle et al, 2003).…”

Section: Relationship Of Soybean To Model Plantsmentioning

confidence: 99%

National Science Foundation-Sponsored Workshop Report. Draft Plan for Soybean Genomics

et al. 2004

View full text Add to dashboard Cite

Recent efforts to coordinate and define a research strategy for soybean (Glycine max) genomics began with the establishment of a Soybean Genetics Executive Committee, which will serve as a communication focal point between the soybean research community and granting agencies. Secondly, a workshop was held to define a strategy to incorporate existing tools into a framework for advancing soybean genomics research. This workshop identified and ranked research priorities essential to making more informed decisions as to how to proceed with large scale sequencing and other genomics efforts. Most critical among these was the need to finalize a physical map and to obtain a better understanding of genome microstructure. Addressing these research needs will require pilot work on new technologies to demonstrate an ability to discriminate between recently duplicated regions in the soybean genome and pilot projects to analyze an adequate amount of random genome sequence to identify and catalog common repeats. The development of additional markers, reverse genetics tools, and bioinformatics is also necessary. Successful implementation of these goals will require close coordination among various working groups.

show abstract

Long-Distance Signaling in Nodulation Directed by a CLAVATA1-Like Receptor Kinase

Cited by 495 publications

References 26 publications

Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase

Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase

Community shifts of soybean stem-associated bacteria responding to different nodulation phenotypes and N levels

National Science Foundation-Sponsored Workshop Report. Draft Plan for Soybean Genomics

Contact Info

Product

Resources

About