Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154

Abstract

Renal carcinoma (RCC) is widely accepted as a malignant tumour of urinary system. Long intergenic non-coding RNA 1939 (LINC01939) is a novel lncRNA which was found to be down-regulated in RCC. Thus, we set out to explore the effect and regulation mechanism of LINC01939 in RCC. LINC01939 and miR-154 in RCC tissues and cell lines were detected using qRT-PCR assay. To examine cellular viability of ACHN and CAKI-1 cells, cell counting kit-8 (CCK-8) assay was exploited here. Flow cytometric analysis was conducted to examine apoptosis. Cell mobility was valued through wound healing assays. Western blotting was applied for examination of proteins related to proliferation, apoptosis, migration and Wnt/ b-catenin/Notch. LINC01939 was down-regulated in RCC tissues. LINC01939 overexpression impeded proliferation and migration, and induced apoptosis. Further study found that the overexpression of LINC01939 strongly suppressed miR-154 expression. Then, the inhibiting effect of overexpressed LINC01939 on proliferation and mobility and the promoting role of LINC01939 in apoptosis were abolished by the combination of miR-154 mimic. Finally, we found that overexpressed LINC01939 inactivated Wnt/b-catenin and Notch through suppressing miR-154. Up-regulation of LINC01939 inhibited proliferation and migration of RCC cells by down-regulating miR-154.

Salvianolic acid A blocks vasculogenic mimicry formation in human non‐small cell lung cancer via PI3K/Akt/mTOR signalling

“…Cell migration was evaluated via wound healing assay as reported previously 26 . Different concentrations of Sal‐A (0–50 µmol/L) were treated with NSCLC cells for 24 hours.…”

The interplay between non-coding RNAs and Wnt/β-catenin signaling pathway in urinary tract cancers: from tumorigenesis to metastasis

“…Functionally, lncRNA NBAT1 functions as a ceRNA for miR-346 to upregulate GSK3β and mitigate cancer cell proliferation, invasion, and metastasis (Xue et al, 2019[ 64 ]). To further investigate the interplay between lncRNAs and miRNAs involved in RCC tumorigenesis, Zhang et al (2020[ 73 ]) reported that the long intergenic ncRNA 1939 (LINC01939) regulates tumor cell growth and progression through downregulating miR-154. The overexpression of LINC01939 inactivates Wnt/β-catenin pathway through suppressing miR-154 in RCC.…”

“…Mechanically, LINC01939 attenuates cancer cell growth and metastasis by downregulating cyclin D1, MMP2, and MMP9. Moreover, LINC01939 was found to induce RCC cell apoptosis through inducing the expression of pro-apoptotic factors including bax and caspase-3 (Zhang et al, 2020[ 73 ]).…”

Long Non-Coding RNAs: A Double-Edged Sword in Renal Cell Carcinoma Carcinogenesis

“…The long intergenic non-coding RNA 1939 (LINC01939) is a novel lncRNA and was first confirmed to be downregulated in gastric cancer and involved in cancer progression 91 . Recently, the latest study indicated that LINC01939 possessed decreased expression in RCC tissues 92 . Upregulation of LINC01939 inhibited the proliferation and migration, as well as induced the apoptosis of ACHN and CAKI-1 cells by decreasing the level of miR-154 with the bluntness of Wnt/$ catenin and Notch 92 .…”

“…Recently, the latest study indicated that LINC01939 possessed decreased expression in RCC tissues 92 . Upregulation of LINC01939 inhibited the proliferation and migration, as well as induced the apoptosis of ACHN and CAKI-1 cells by decreasing the level of miR-154 with the bluntness of Wnt/$ catenin and Notch 92 .…”