Long Non-coding Antisense RNA TNRC6C-AS1 Is Activated in Papillary Thyroid Cancer and Promotes Cancer Progression by Suppressing TNRC6C Expression

Muhanhali, Dilidaer; Zhai, Tianyu; Jiang, Jingjing; Ai, Zhilong; Zhu, Wei; Ling, Yan

doi:10.3389/fendo.2018.00360

Cited by 19 publications

(18 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One predicted apoptosis-associated lncRNA TNRC6C-AS1 was recently reported to powerfully modulate cell apoptosis. After down-regulation of TNRC6C-AS1 by transfecting siRNA in papillary thyroid cancer derived cell lines TPC1, apoptosis of TPC1 cells were significantly increased when compared to the control in an apoptosis assay by flow cytometry [ 52 ]. LncRNAs MIR17HG and LINC00263 were predicted to be involved in cell proliferation and growth (hallmark "Sustaining proliferative signaling") and LINC00263 also contributes to hallmark "Evading growth suppressors".…”

Section: Resultsmentioning

confidence: 99%

Capturing functional long non-coding RNAs through integrating large-scale causal relations from gene perturbation experiments

Long

et al. 2018

EBioMedicine

View full text Add to dashboard Cite

Characterizing functions of long noncoding RNAs (lncRNAs) remains a major challenge, mostly due to the lack of lncRNA-involved regulatory relationships. A wide array of genome-wide expression profiles generated by gene perturbation have been widely used to capture causal links between perturbed genes and response genes. Through annotating >600 gene perturbation profiles, over 354,000 causal relationships between perturbed genes and lncRNAs were identified. This large-scale resource of causal relations inspired us to develop a novel computational approach LnCAR for inferring lncRNAs' functions, which showed a higher accuracy than the co-expression based approach. By application of LnCAR to the cancer hallmark processes, we identified 38 lncRNAs involved in distinct carcinogenic processes. The “activating invasion & metastasis” related lncRNAs were strongly associated with metastatic progression in various cancer types and could act as a predictor of cancer metastasis. Meanwhile, the “evading immune destruction” related lncRNAs showed significant associations with immune infiltration of various immune cells and, importantly, can predict response to anti-PD-1 immunotherapy, suggesting their potential roles as biomarkers for immune therapy. Taken together, our approach provides a novel way to systematically reveal functions of lncRNAs, which will be helpful for further experimental exploration and clinical translational research of lncRNAs.

show abstract

Section: Resultsmentioning

confidence: 99%

Capturing functional long non-coding RNAs through integrating large-scale causal relations from gene perturbation experiments

Long

et al. 2018

EBioMedicine

View full text Add to dashboard Cite

show abstract

“…21 Meanwhile, a new study has showed the influence of lncRNA TNRC6C-AS1 on proliferation, apoptosis and invasion of papillary TC cells. 8 In this study, we explored the role of the lncRNA TNRC6C-AS1, STK4…”

Section: Discussionmentioning

confidence: 99%

“…LncRNA TNRC6C-AS1 has been reported to play a tumorigenic role in the tumour occurrence and invasive ability of papillary thyroid carcinoma (PTC). 8 A new study has found that lncRNA TNRC6C-AS1 affects the development of TC by regulating UNC5B expression as a competitive endogenous RNA of microRNA-129-5p (miR-129-5p). 9 The complementary pairing sites in the promoter regions of lncRNA TNRC6C-AS1 and serine/ threonine-protein kinase 4 (STK4) genes were analysed by bioinformatics.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, lncRNA TNRC6C‐AS1 was the main focus of our study. LncRNA TNRC6C‐AS1 has been reported to play a tumorigenic role in the tumour occurrence and invasive ability of papillary thyroid carcinoma (PTC) . A new study has found that lncRNA TNRC6C‐AS1 affects the development of TC by regulating UNC5B expression as a competitive endogenous RNA of microRNA‐129‐5p (miR‐129‐5p) .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Long non‐coding RNA TNRC6C‐AS1 promotes methylation of STK4 to inhibit thyroid carcinoma cell apoptosis and autophagy via Hippo signalling pathway

Peng

Tan

et al. 2019

J Cellular Molecular Medi

View full text Add to dashboard Cite

The role of long non‐coding RNAs (lncRNAs) in thyroid carcinoma (TC), the most frequent endocrine malignancy, has been extensively examined. This study investigated effect of interaction among lncRNA TNRC6C‐AS1, serine/threonine‐protein kinase 4 (STK4) and Hippo signalling pathway on TC. Initially, lncRNA TNRC6C‐AS1 expression in TC tissues was detected. To explore roles of lncRNA TNRC6C‐AS1, STK4 and Hippo signalling pathway in TC progression, their expressions were altered. Interaction between lncRNA TNRC6C‐AS1 and STK4, STK4 promoter methylation, or Hippo signalling pathway was verified. After that, a series of experiments were employed to evaluate in vitro ability of apoptosis, proliferation and autophagy of TC cells and in vivo tumorigenicity, and tumour growth of TC cells. lncRNA TNRC6C‐AS1 was highly expressed while STK4 was poorly expressed in TC tissues. LncRNA TNRC6C‐AS1 promoted the STK4 methylation and down‐regulated STK4 expression, which further activated the Hippo signalling pathway. STK4 silencing was observed to promote the proliferation ability of TC cells, inhibit the apoptosis and autophagy abilities, as well as enhance the tumorigenicity and tumour growth. Moreover, the in vitro proliferation ability as well as the in vivo tumorigenicity and tumour growth of TC cells were inhibited after the blockade of Hippo signalling pathway, while the apoptosis and autophagy abilities were promoted. The results demonstrate that the lncRNA TNRC6C‐AS1 increases STK4 promoter methylation to down‐regulate STK4 expression, thereby promoting the development of TC through activation of Hippo signalling pathway. It highlights that lncRNA TNRC6C‐AS1 may be a novel therapeutic target for the treatment of TC.

show abstract

“…In thyroid cancer several lncRNAs were shown to have pathogenic and predictive role, including BANCR, FALEC, CNALPTC1, PVT1, NAMA, PTCSC1, PTCSC2, PTCSC3, TNRC6C-AS1 and others [10][11][12][13][14][15][16][17][18][19][20][21].…”

mentioning

confidence: 99%

Long non-coding RNA landscapes in benign and malignant thyroid neoplasms of distinct histological subtypes

Yakushina

Лавров

2019

Preprint

View full text Add to dashboard Cite

Background: The main types of thyroid neoplasms, follicular adenoma (FA), follicular thyroid carcinoma (FTC), classical and follicular variants of papillary carcinoma (clPTC and fvPTC), anaplastic thyroid carcinoma (ATC), are differ in the prognosis, rate of progression and metastatic behavior. It can be supposed that there are specific patterns of lncRNAs involved in the development of clinical and morphological features. The lncRNA landscapes within distinct benign and malignant histological variants of thyroid neoplasm are unknown. Methods: Comprehensive set of Microarray and RNA-Seq datasets was analyzed for the expression of lncRNAs in FA, FTC, fvPTC, clPTC and ATC. The potential biological functions were evaluated via coexpression and enrichment analysis. Results and conclusion: Abberant expression of lncRNA in FA, FTC, fvPTC, clPTC and ATC was established. The lncRNAs common for benign and malignant neoplasms, specific for papillary carcinomas, specific for clPTC, fvPTC and ATC are determined. The determined common and specific lncRNAs are found to be putatively involved into L1CAM interactions; processing of capped intron-containing pre-mRNA; Tryptophan metabolism; PCP/CE pathway and Beta-catenin independent WNT signaling; extracellular matrix organization and cell cycle and mitotic. The patterns of lncRNA expression in FA and FTC are appeared to be similar with no genes significantly differentially expressed within these subtypes. Previously known oncogenic and supressor lncRNAs (NR2F1-AS1, LINC00511, SLC26A4-AS1, CRNDE, LINC01116, RMST) are found aberrantly expressed in thyroid carcinomas. The findings enhance the understanding of lncRNA landscape in thyroid neoplasms and its role in thyroid cancer progression.

show abstract

Long Non-coding Antisense RNA TNRC6C-AS1 Is Activated in Papillary Thyroid Cancer and Promotes Cancer Progression by Suppressing TNRC6C Expression

Cited by 19 publications

References 34 publications

Capturing functional long non-coding RNAs through integrating large-scale causal relations from gene perturbation experiments

Capturing functional long non-coding RNAs through integrating large-scale causal relations from gene perturbation experiments

Long non‐coding RNA TNRC6C‐AS1 promotes methylation of STK4 to inhibit thyroid carcinoma cell apoptosis and autophagy via Hippo signalling pathway

Long non-coding RNA landscapes in benign and malignant thyroid neoplasms of distinct histological subtypes

Contact Info

Product

Resources

About