Long non‑coding RNA 00152 functions as a competing endogenous RNA to regulate NRP1 expression by sponging with miRNA‑206 in colorectal cancer

Abstract

Colorectal cancer (CRC) is the third most common type of cancer; however, the molecular mechanisms underlying colorectal tumor metastasis and growth remain elusive. Recently, accumulating evidence has indicated that long non‑coding RNAs (lncRNAs) play a critical role in CRC progression and metastasis; however, the biological role and clinical significance of lncRNA 00152 (lnc00152) in CRC remains largely unknown. Thus, in this study, lnc00152 expression was measured in 80 human CRC tissue samples, 40 non‑cancerous tissue samples, and 3 CRC cell lines (SW480, SW620 and LoVo) using RT‑qPCR. We examined the effects of lnc00152 on CRC cells following transfection with lnc00152 overexpression plasmid or respective siRNA in vitro and in vivo. Luciferase assays revealed the mechanism driving competitive endogenous RNA (ceRNA). We identified that lnc00152 was aberrantly overexpressed in colorectal tumors and cancer cells and that lnc00152 was modulated by miRNA‑206. lnc00152 overexpression enhanced the proliferative and invasive ability of CRC cells in vitro, promoted tumor growth in vivo, and was associated with the shorter overall survival of patients with CRC. In addition, lnc00152 overexpression promoted epithelial-mesenchymal transition (EMT) and increased neuropilin‑1 (NRP1) expression in the CRC cells. By contrast, lnc00152 silencing exerted a counteractive effect. Collectively, these findings demonstrate the critical role of lnc00152 in tumor growth and progression in CRC, and identify a novel therapeutic target associated with CRC development and progression.

Identify CRNDE and LINC00152 as the key lncRNAs in age-related degeneration of articular cartilage through comprehensive and integrative analysis

“…LINC00152 is an 828 bp lncRNA located on chromosome 2p11.2. Previous studies have found that LINC00152 plays an oncogenic role in the development of a wide range of tumor types (Chen et al, 2018a; Chen et al, 2018c). Enhanced LINC00152 expression has also been found to be a potential prognostic biomarker in patients with lung and colorectal cancers (Chen et al, 2018b; Li et al, 2017b).…”

Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p/RAI14 axis

“…LncRNA PVT1 enhances the viability and invasion of papillary thyroid carcinoma cells by functioning as ceRNA of microRNA-30a to mediate the expression of insulin like growth factor 1 receptor [29]. LncRNA 00152 functions as a ceRNA to regulate NRP1 expression by sponging miRNA-206 in colorectal cancer [30]. Through bioinformatics tools and (See figure on previous page.)…”

Neuropilins in the Context of Tumor Vasculature

“…Therefore, they play a major role in all stages of tumorigenesis and metastasis by [263]. In colorectal cancer, miR-206 inhibits tumor growth and invasion by downregulating the long non-coding RNA lnc00152 which again promotes NRP1 expression and EMT [264]. In addition, miRs can also mutually regulate their activity.…”