Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer

Abstract

Background and objectiveLong non-coding RNAs can regulate tumorigenesis of various cancers. Dys-regulation of lncRNA-AFAP1-AS1 has not been studied in colorectal carcinoma (CRC). This study was to examine the function involvement of AFAP1-AS1 in tumor growth and metastasis of CRC.MethodsRelative expression of AFAP1-AS1 in CRC tissues and CRC cells lines was determined using quantitative real-time PCR (qRT-PCR). Functional involvement of AFAP1-AS1 in tumor proliferation and metastasis was evaluated in AFAP1-AS1-specific siRNA-treated CRC cells and in CRC cell xenograft. Expression of epithelial-mesenchymal transition (EMT)-related gene expression was determined using western blot.ResultsRelative expression of AFAP1-AS1 was significantly elevated in CRC tissues and CRC HCT116 and SW480 cell lines. AFAP1-AS1 knock-down suppressed SW480 cell proliferation, colony formation, migration and invasion. Also AFAP1-AS1 knock-down inhibited tumor metastasis-associated genes expression in terms of EMT. This carcinostatic action by AFAP1-AS1 knock-down was further confirmed by suppression of tumor formation and hepatic metastasis of CRC cells in nude mice.ConclusionlncRNA-AFAP1-AS1 knock-down exhibits antitumor effect on colorectal carcinoma in respects of suppression of cell proliferation and metastasis of cancer cells.

Long Noncoding RNA AFAP1-AS1 Promoted Tumor Growth and Invasion in Cholangiocarcinoma

“…It is well known that aberrant MMP-2 and MMP-9 expression was involved in metastasis [28]. As we expected, the findings of our study showed that AFAP1-AS1 silencing resulted in a significant decrease in cell migration and invasion with downregulation of MMP-2 and MMP-9, which was consistent with others reports [23, 29]. …”

Overexpression long non-coding RNA LINC00673 is associated with poor prognosis and promotes invasion and metastasis in tongue squamous cell carcinoma

“…After siRNA-mediated silencing of LINC00673 , we found that could significantly inhibit TSCC cancer cell invasive and migratory abilities. In recent findings, some lncRNAs, such as actin filament associated protein 1 antisense RNA1 ( AFAP1-AS1 ) [44, 45], HOX transcript antisense RNA ( HOTAIR ) [46], growth-arrest-specific transcript 5 ( GAS5 ) [47], have been reported to regulate cancer metastasis process by multiple ways. The potential role of LINC00673 abnormalities in TSCC is worth for further investigated.…”

AFAP1‐AS1: A novel oncogenic long non‐coding RNA in human cancers

“…30,35,[40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58] In this review, the related clinicopathological characteristics and molecular functions of this lncRNA in cancers are presented in Tables 1 and 2.…”