Long non-coding RNA AGAP2-AS1 increases the invasiveness of papillary thyroid cancer

Abstract

Papillary thyroid cancer (PTC) is considered a low hazard endocrine system cancer, but a considerable number of patients have poor prognosis because of lymph node metastasis and invasion of surrounding tissues. In this study, we analyzed the expression and function of the long non-coding RNA (lncRNA) AGAP2-AS1 in PTC. We found that AGAP2-AS1 expression was significantly higher in human PTC tissues than adjacent noncancerous tissues (n=110; p<0.01) and correlated with lymph node metastasis (p=0.01) and tumor-node-metastasis stage (p=0.006). AGAP2-AS1 downregulation decreased migration and invasion by PTC cells, and reduced expression of matrix metalloproteinase-2 (MMP2). AGAP2-AS 1 upregulated MMP2 expression by competitively binding to microRNA-425-5p. In addition, miR-424-5p expression was decreased in PTC tissues and correlates negatively with the AGAP2-AS1 levels. These results demonstrate that AGAP2-AS1 expression is significantly elevated in PTC tissues and that, by binding to miRNA-425-5p, it upregulates the MMP2 expression, thereby increasing the invasiveness and migration capacity of PTC cells.

The lncRNA CASC9 alleviates lipopolysaccharide-induced acute kidney injury by regulating the miR-424-5p/TXNIP pathway

“… 38 Previous studies illustrated that miR-93-5p can be packaged in extracellular vesicles and secreted by endothelial progenitor cells to alleviate sepsis-induced AKI. 11 miR-424-5p also plays an important role in muscle development 39 and tumor progression in cancers including papillary thyroid cancer, non-small-cell lung cancer, and cervical cancer, 40 – 42 but its role in S-AKI remains to be elucidated. Our study suggests that miR-424-5p is a target of CASC9, and the interaction between miR-424-5p and CASC9 is involved in regulating LPS-induced cell damage.…”

The prognostic value of lncRNA AGAP2-AS1 in cancer patients

“…All of the included studies were published between 2016 and 2020 and came from China. Multiple forms of cancers were analyzed in the present meta-analysis, including pancreatic cancer, [ 11 ] hepatocellular carcinoma, [ 15 ] glioblastoma, [ 16 ] gastric cancer, [ 12 ] nonsmall cell lung cancer (NSCLC), [ 9 , 17 ] colorectal cancer (CRC), [ 18 ] epithelial ovarian cancer, [ 19 ] papillary thyroid cancer, [ 20 ] glioma. [ 21 ] The detailed information obtained from the studies is summarized in Table 1 .…”

“…Region Tumor type Sample size TNM stage High Low Cutoff value Detection method Outcome measure NOS Hui et al 2019 [11] China PC 46 I-IV 23 23 Median qRT-PCR OS 6 Liu et al 2019 [15] China HCC 137 I-IV 69 68 Median qRT-PCR OS, DFS 7 Tian et al 2018 [16] China GBM 40 NA 20 20 Median qRT-PCR OS 7 Qi et al 2017 [12] China GC 50 I-IV 25 25 Median qRT-PCR OS, PFS 8 Li et al 2016 [17] China NSCLC 80 NA 40 40 Median qRT-PCR OS, DFS 6 Hong et al 2020 [18] China CRC 116 I-IV 58 58 Median qRT-PCR OS, DFS 7 Fan et al 2017 [9] China NSCLC 198 I-IV 99 99 Median qRT-PCR OS 8 Tingting et al 2020 [19] China EOC 80 NA 40 40 Median qRT-PCR NA 8 Shao et al 2020 [20] China PTC 110 I-IV 55 55 Median qRT-PCR NA 8 Zheng et al 2019 [21] China…”

Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines

“…Concerning miR-425, upregulated by A FPE in CAL62 cells, in literature is described to be decreased in PTC tissues; furthermore, the transfection of miR-425 mimic decreases the motility phenotype of PTC cells [ 66 ]. Interestingly, the aberrant expression of miR-425 is also correlated to the oxidative stress in breast cancer cell lines where it regulates the apoptosis by PTEN axis [ 67 , 68 ].…”