Long Non-coding RNA CRNDE Exacerbates NPC Advancement Mediated by the miR-545-5p/CCND2 Axis

Abstract

Background: Previous studies indicated CRNDE to have a pivotal part within tumorigenesis. Notwithstanding, precise details on CRNDE activities within NPC are still uncertain. The investigation described in this article served to focus in greater depth on the mechanistics regarding CRNDE, together with all associated regulatory networks, on nasopharyngeal carcinoma (NPC) and its treatment possibilities.Methods：Quantitative real-time polymerase chain reaction (RT-qPCR) analyzed CRNDE, miR-545-5p and CCND2 expression within NPCs and representative cell lineages. CCK-8 cell counting-, EdU-, wound-healing- / transwell-assays analyzed cellular proliferation, migrative, together with invasive properties. Apoptosis / cell cycle progression were scrutinized through flow cytometry. Dual-luciferase reporter assays validated CRNDE / miR-545-5p / CCND2 interplay. Proteomic expression of apoptosis-related protein, EMT-related protein and CCND2 protein were evaluated through Western blotting. In addition, Ki67 expression was evaluated through immunohistochemical staining. The effect of CRNDE in vivo was assessed by nude murine xenograft model studies.Results: This study demonstrated up-regulated expression of CRNDE and CCND2 within NPC tissues /cell lines. Meanwhile, miR-545-5p was downregulated. CRNDE knockdown or miR-545-5p over-expression drastically reduced NPC proliferative, migrative and invasive properties, promoted apoptosis / altered cell cycle, and inhibited the expression of CCND2. However, miR-545-5p downregulation had opposing effects. All inhibiting functions generated by CRNDE downregulation upon NPC progression could be counterbalanced or synergistically exacerbated, depending on miR-545-5p downregulation or upregulation, respectively. Multiple-level investigations revealed CRNDE to serve as a sponge for miR-545-5p and can target CCND2 within NPCs.Conclusions：CRNDE increases CCND2 expression by competitive binding with miR-545-5p, thus accelerating the development of NPC. This provides potential therapeutic targets and prognostic markers against NPC.