Long non‑coding RNA GACAT3 promotes liver cancer progression by regulating the proliferation, apoptosis and migration of tumor cells

Abstract

Long non-coding RNA gastric cancer associated transcript 3 (GACAT3), is a newly identified non-coding RNA, which has been found to be involved in the tumorigenesis of gastric cancer. However, the biological function of GACAT3 in liver cancer remains unclear. The present study aimed to determine the expression level and function of GACAT3 in liver cancer. The authors cultured liver cancer cells in vitro and GACAT3 was silenced in the cells. Cell proliferation, apoptosis and migration were determined by MTT assay, flow cytometric analysis and transwell assay, respectively. It was demonstrated that GACAT3 was upregulated in liver cancer tissues. The inhibition of GACAT3 decreased the ability of hepatoma cells to proliferate and migrate, and increased apoptosis of the cells. These findings provide the first evidence, to the best of our knowledge, of the exact role of GACAT3 in liver cancer, suggesting GACAT3 as a potential target for liver cancer therapy in the future.

LncRNA GACAT3 contributes to osteoarthritis progression by suppressing growth and inducing apoptosis of chondrocytes through miR-195/TGF-β/Smad5 axis

“…The effects were also supported by the results of western blot analysis, in which GACAT3 suppressed the expression of PCNA while enhanced the cleaved caspase 3 and cleaved PAPR. These results were consistent with that from studies on tumours [32,27]. Thus, we speculated that GACAT3 was involved in the OA pathology, and meanwhile its upregulation in chondrocytes may promote OA progression.…”

LncRNA GACAT3: A Promising Biomarker and Therapeutic Target in Human Cancers

“…Hepatocellular carcinoma (HCC) is the most common type of liver cancer ( Anwanwan et al, 2020 ), and overexpression of many lncRNAs in hepatocytes contributes to carcinogenesis in HCC ( Wang et al, 2017 ; Feng et al, 2019 ; Zhang et al, 2019 ; Zhang et al, 2020b ). GACAT3 is aberrantly overexpressed in HCC tissues and liver cancer cell lines, including HepG2, HCCLM3, SK-Hep-1, SMMC-7721, and Huh7 ( Dong et al, 2020 ; Li et al, 2020 ). Importantly, GACAT3 is associated with lower OS and disease-free survival in HCC patients ( Dong et al, 2020 ).…”

LncRNA LINC01535 promotes colorectal cancer development and chemoresistance by sponging miR‑761

“…Tumor xenograft experiment. HCT116 cells (2x10 6 ) stably transfected with sh-LINC01535 or sh-NC were subcutaneously injected subcutaneously into the left flank of 6-week-old female nude mice (n=5 mice per group; weight, ~20 g). For the animal study, humane endpoints were utilized to prevent unnecessary animal suffering according to the Guide for the Care and Use of laboratory Animals from the National Institutes of Health.…”

“…Long noncoding RNA (lncRNA) is a class of transcripts with >200 nucleotides in length (4,5). Accumulating evidence has indicated that lncRNAs have crucial roles in various biological processes (6)(7)(8). Furthermore, emerging studies have suggested that lncRNAs have important roles in the initiation and progression of human cancers (9,10).…”