Long non‑coding RNA heart and neural crest derivatives expressed�2‑antisense RNA�1 overexpression inhibits the proliferation of cancer cells by reducing RUNX2 expression in triple‑negative breast cancer

Abstract

The role of long non-coding RNA heart and neural crest derivatives expressed 2-antisense RNA 1 (lncRNA HAND2-AS1) in tumor suppression has been reported in a number of cancer types, while its functionality in triple-negative breast cancer (TNBC) remains unclear. The present study aimed to investigate the involvement of lncRNA HAND2-AS1 in TNBC using different methodologies. HAND2-AS1 and RUNX2 expression was analyzed using reverse transcription-quantitative PCR and western blot analysis. Diagnostic analysis was performed using receiver operating characteristic curve. Overexpression experiments were performed to analyze the interaction between HAND2-AS1 and RUNX2 while the Cell Counting Kit-8 assay was performed to analyze cell proliferation. In patients with early-stage TNBC, the expression level of lncRNA HAND2-AS1 was downregulated, whilst runt-related transcription factor 2 (RUNX2) mRNA was upregulated in tumor tissues, compared with paired healthy tissues. Expression levels of lncRNA HAND2-AS1 and RUNX2 mRNA were inversely correlated in tumor tissues, but not in paired healthy tissues. Decreased plasma expression levels of lncRNA HAND2-AS1 were observed in TNBC patients compared with those in healthy controls, and the downregulation of lncRNA HAND2-AS1 distinguished patients with TNBC from healthy controls. lncRNA HAND2-AS1 overexpression inhibited RUNX2 expression, whilst RUNX2 overexpression did not significantly affect lncRNA HAND2-AS1 expression in the MDA-MB-231 and BT-20 cell lines. lncRNA HAND2-AS1 overexpression resulted in the inhibition of cell proliferation, while RUNX2 overexpression promoted the proliferation of TNBC cells. RUNX2 overexpression partially reversed the effects of lncRNA HAND2-AS1 overexpression on cancer cells. Therefore lncRNA HAND2-AS1 may inhibit the proliferation of cancer cells by inhibiting RUNX2 expression in TNBC.

HAND2-AS1 Works as a ceRNA of miR-3118 to Suppress Proliferation and Migration in Breast Cancer by Upregulating PHLPP2

“…In this study, we mainly analyzed HAND2-AS1 and discovered that it is downregulated in BC cell lines, which was in accordance with previous reports [ 19 , 20 ]. In addition, HAND2-AS1 could suppress proliferation, migration, and invasion but accelerated death in BC cells, which was consistent with the findings in the previous study about the HAND2-AS1 role in cancers [ 20 , 21 ]. In addition, there has not been research involving the effect of HAND2-AS1 in a breast cancer animal model.…”

Comprehensive Analysis of Molecular Clusters and Prognostic Signature Based on m7G-related LncRNAs in Esophageal Squamous Cell Carcinoma

“…In Jin et al reported that lncRNA HAND2-AS1 expression was reduced in patients with cervical cancer compared to normal subjects and that it inhibited the proliferation, migration and invasion of cancer cells by downregulating ROCK1 (13). In a study by Min et al, patients with Triple-negative breast cancer (TNBC) had significantly lower plasma levels of lncRNA HAND2-AS1 and were able to act as oncogenes in TNBC by downregulating RUNX 2 to inhibit cancer cell proliferation compared to healthy controls (14). Small nucleolar RNA host gene 7 (SNHG7) is a novel oncogenic lncRNA located on chromosome 9q34.3, which is 2,176 bp long (15).…”

LncRNA HAND2-AS1 suppressed the growth of triple negative breast cancer via reducing secretion of MSCs derived exosomal miR-106a-5p

“…Herein, we predicted that a binding between miR-106a-5p and HAND2-AS1. And it has been reported that HAND2-AS1 was involved in the progression of breast cancer [ 31 , 32 ]. And our results showed HAND2-AS1 acted as a sponge of miR-106a-5p, and HAND2-AS1 was decreased in TNBC tissues and cells.…”