Long non-coding RNA HOTAIR modulates HLA-G expression by absorbing miR-148a in human cervical cancer

Abstract

Abstract. The long non-coding RNA HOX transcript antisense RNA (HOTAIR) has been found overexpressed in many human malignancies and involved in tumor progression and metastasis. However, little is known about the potential biological roles of HOTAIR in tumor escape. In the present study, the expression of HOTAIR was detected in 59 paired cervical cancer tissue samples by real-time PCR and then subjected to correlation analysis with clinical features. The effects of HOTAIR on cervical cancer cells as well as the expression of human leukocyte antigen (HLA)-G were studied by overexpression and RNA interference approaches. Insight into the mechanism of HOTAIR acting as competitive endogenous RNAs (ceRNAs) was gained from bioinformatic analysis and luciferase assays. HOTAIR expression was obviously increased in cervical cancer tissue. HOTAIR upregulation was associated with advanced pathological stage, histology, lymph node invasion and lymphatic metastasis, and also correlated with shorter overall survival of cervical cancer patients. Furthermore, HOTAIR overexpression promoted the proliferation, migration and invasion of cervical cancer cells, while HOTAIR knockdown inhibited cell invasion and cell viability, induced apoptosis and inhibited growth in vitro and in vivo. Moreover, HOTAIR modulated human leucocyte antigen-G (HLA-G) expression by competitively binding miR-148a. Our data suggest that HOTAIR plays an important oncogenic role in cervical cancer and might serve as a marker for cervical cancer prognosis and a potential target for therapeutic intervention.

Long Non-coding RNAs: Emerging Roles in the Immunosuppressive Tumor Microenvironment

“…This study investigated the ability of HOTAIR in differentiating benign thyroid nodules from malignant nodules (NCT03469544). As reviewed above, HOTAIR upregulates the expression of HLA-G in tumor tissues (47,57), which indicated the potential role of HOTAIR as a biomarker of tumor immune escape. Other lncRNAs that regulate the pivotal molecules and pathways that are targeted in clinical therapies, such as tumor vaccine, T cellbased therapy, and ICB, can also potentially predict response to tumor immunotherapy.…”

HOTAIR: An Oncogenic Long Non-Coding RNA in Human Cancer

“…Loss-of-function experiments indicated that overexpression of HOTAIR promoted, whereas silencing of HOTAIR inhibited growth, invasion, and cell viability of CaCx cells in vitro and in vivo. Moreover, HOTAIR modulated the expression of human leucocyte antigen-G by competitively binding to miR-148a; this effect suggests that HOTAIR played an important oncogenic role in CaCx and might serve as a biomarker and a potential target to determine prognosis, as well as a therapeutic intervention for CaCx [96]. Pearson's correlation analysis indicated the negative correlation between the expression levels of miR-17-5p and HOTAIR.…”

“…Elevated HOTAIR was associated with advanced tumor stage, lymphatic node metastasis, recurrence, and short OS. Thus, circulating HOTAIR may be used as a potent prognostic marker in patients with CaCx [94][95][96]. Loss-of-function experiments indicated that overexpression of HOTAIR promoted, whereas silencing of HOTAIR inhibited growth, invasion, and cell viability of CaCx cells in vitro and in vivo.…”

Long non-coding RNA LOC100507600 functions as a competitive endogenous RNA to regulate BMI1 expression by sponging miR128-1-3p in Hirschsprung's disease

“…As is known, lncRNAs can also function as miRNA sponge in thyroid cancer [27], cervical cancer [28] and gastric cancer [29]. We speculated that LOC100507600 might competitive bind to the miR128-1-3p, thereby stopping its target RNA from suppression.…”