Long non-coding RNA HOTAIR promotes HLA-G expression via inhibiting miR-152 in gastric cancer cells

Song, Bingtan; Guan, Zhongzheng; Liu, Fengjun; Sun, Dong; Wang, Kexin; Qu, Hui

doi:10.1016/j.bbrc.2015.07.040

Cited by 76 publications

(55 citation statements)

References 26 publications

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“…Moreover, accumulating studies have demonstrated the aberrant expression of lncRNAs in various human tumors, including colorectal cancer [17], liver cancer [18], breast cancer [19]and lung cancer [20]. For instance, lncRNA HOTAIR (Hox transcript antisense intergenic RNA) controls gene expression by interacting with a Polycomb Repressive Complex 2 (PRC2) and high levels of HOTAIR expression was investigated in multiple tumors pathogenesis and progression [21–23]. Metastasis-associated lung adenocarcinoma transcript1 (MALAT1) was firstly characterized as one of the cancer-associated lncRNAs that played crucial roles in several cancer types [24–26].…”

Section: Discussionmentioning

confidence: 99%

Knockdown of long non-coding RNA CCAT2 suppressed proliferation and migration of glioma cells

Guo

Yang

et al. 2016

Oncotarget

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Long non-coding RNA colon cancer-associated transcript 2 (CCAT2) is commonly investigated in a number of cancers. However, little is known of its expression and biological function in glioma biology. In the current study, we used quantitative real-time PCR (qRT-PCR) to determine the expression of CCAT2 in glioma tissues. We found that expression of CCAT2 was up-regulated in glioma tissues and significantly correlated with the advanced tumor stage (III/IV). Functional assays in vitro and in vivo demonstrated that knockdown of CCAT2 could inhibit proliferation, cell cycle progression and migration of glioma cells. Further analysis indicated the effect of CCAT2 knockdown on glioma cell phenotype through inhibiting Wnt/β-catenin signal pathway activity. Thus, our study provides evidence that CCAT2 may function as a potential biomarker for glioma.

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Section: Discussionmentioning

confidence: 99%

Knockdown of long non-coding RNA CCAT2 suppressed proliferation and migration of glioma cells

Guo

Yang

et al. 2016

Oncotarget

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“…As some authors have reported, long noncoding RNAs (lncRNAs) can inhibit or stimulate miRNA and regulate certain physiological processes. 23,24 Although the repertoire of lncRNAs remains to be elucidated, a growing body of evidence suggests that they are important regulators of a diverse array of cellular function. 25 Thus, in future research, we need to describe the expression profile and functions of lncRNAs in HBSMCs.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA 4323 induces human bladder smooth muscle cell proliferation under cyclic hydrodynamic pressure by activation of erk1/2 signaling pathway

Sun

Luo

Zhu

et al. 2016

Exp Biol Med (Maywood)

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We cultivated human bladder smooth muscle cells (HBSMCs) under pressures of 0 or 200 cm H 2 O pressure for 24 h, before using microarray technology to extract and analyze the different expressions of miRNAs and mRNAs in the two groups. We also predicted the target mRNA of the miDNA and performed functional forecasting. Changes in miRNA were identified by quantitative real-time polymerase chain reaction (qRT-PCR) after overexpressing miRNA by transfection. We used flow cytometry to examine HBSMC proliferation, and we used qRT-PCR and Western blot analyses to quantify the expression and activation of mRNAs and proteins. There were nine upregulated and four downregulated miRNAs involved in cell proliferation, including miR 4323, which was identified by qRT-PCR ( p ¼ 0.027). In addition, miR 4323 was shown to inhibit LYN ( p ¼ 0.031), decrease lyn kinase ( p ¼ 0.037), and promotes the phosphorylation of extracellular regulated protein kinases 1 and 2 (Erk1/2) (p ¼ 0.004). Moreover, overexpression of miR 4323 activated the proliferation pathway regulated by Erk1/2. Then, miR 4323 was shown to stimulate the proliferation of HBSMCs, with the proliferation index improving from 30.84 AE 4.57 to 52.13 AE 3.41 ( p ¼ 0.001). In summary, when the miRNA miR 4323 was overexpressed under cyclic hydrodynamic pressure, LYN is decreased and the Erk1/2 signaling pathway is activated; in addition, miR 4323 is involved in HBSMC proliferation when under hydrodynamic pressure.

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“…Moreover, also miR-1 was able to negatively regulate HOTAIR expression, thus generating a reciprocal repression feedback loop between these two ncRNAs [118]. Other experimental evidence showed that HOTAIR was capable of binding and downregulating miR-152 in gastric cancer [119]. HOTAIR overexpression in gastric cancer tissues led to decreased expression of miR-152 and to upregulation of its target, HLA-G (human leukocyte antigen G), which in turn facilitated tumor escape mechanisms [119].…”

Section: Noncoding Rnas: Different Ways To Interplay Among Each Othermentioning

confidence: 99%

Molecular Crosstalking among Noncoding RNAs: A New Network Layer of Genome Regulation in Cancer

Ragusa

Barbagallo

Brex

et al. 2017

International Journal of Genomics

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Over the past few years, noncoding RNAs (ncRNAs) have been extensively studied because of the significant biological roles that they play in regulation of cellular mechanisms. ncRNAs are associated to higher eukaryotes complexity; accordingly, their dysfunction results in pathological phenotypes, including cancer. To date, most research efforts have been mainly focused on how ncRNAs could modulate the expression of protein-coding genes in pathological phenotypes. However, recent evidence has shown the existence of an unexpected interplay among ncRNAs that strongly influences cancer development and progression. ncRNAs can interact with and regulate each other through various molecular mechanisms generating a complex network including different species of RNAs (e.g., mRNAs, miRNAs, lncRNAs, and circRNAs). Such a hidden network of RNA-RNA competitive interactions pervades and modulates the physiological functioning of canonical protein-coding pathways involved in proliferation, differentiation, and metastasis in cancer. Moreover, the pivotal role of ncRNAs as keystones of network structural integrity makes them very attractive and promising targets for innovative RNA-based therapeutics. In this review we will discuss: (1) the current knowledge on complex crosstalk among ncRNAs, with a special focus on cancer; and (2) the main issues and criticisms concerning ncRNAs targeting in therapeutics.

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Long non-coding RNA HOTAIR promotes HLA-G expression via inhibiting miR-152 in gastric cancer cells

Cited by 76 publications

References 26 publications

Knockdown of long non-coding RNA CCAT2 suppressed proliferation and migration of glioma cells

Knockdown of long non-coding RNA CCAT2 suppressed proliferation and migration of glioma cells

MicroRNA 4323 induces human bladder smooth muscle cell proliferation under cyclic hydrodynamic pressure by activation of erk1/2 signaling pathway

Molecular Crosstalking among Noncoding RNAs: A New Network Layer of Genome Regulation in Cancer

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