Long non-coding RNA HOXA-AS3 promotes cell proliferation of oral squamous cell carcinoma through sponging microRNA miR-218-5p

Abstract

Increasing evidence demonstrated long non-coding RNAs (lncRNAs) play important roles in the occurrence and development of oral squamous cell carcinoma (OSCC). This study aimed to explore the role and molecular mechanism of lncRNA HOXA-AS3 in the progression of OSCC. Here, we found that t he expression of lncRNA HOXA-AS3 was upregulated in OSCC tissues and cell lines compared with the para-cancerous tissues and normal human oral keratinocyte (NHOK), respectively. Inhibition of HOXA-AS3 significantly inhibited the proliferation and colony formation of OSCC cells. Further, the luciferase reporter assay showed that HOXA-AS3 was directly bound to miR-218-5p. Moreover, the expression of miR-218-5p was negatively regulated by HOXA-AS3, and miR-218-5p could inhibit the expression of collagen type I alpha1 (COL1A1) and lysophosphatidylcholine acyltransferase 1 (LPCAT1). In addition, silencing miR-218-5p reversed the inhibitory effect of HOXA-AS3 knockdown on the proliferative potential of OSCC cells. In summary, our study illustrated that HOXA-AS3 promoted cancer cell proliferation in OSCC, possibly by sponging miR-218-5p for the first time, which provides a new target or a potential diagnostic biomarker for OSCC.

Downregulation of circLPAR3 inhibits tumor progression and glycolysis by liberating miR‐144‐3p and upregulating LPCAT1 in oral squamous cell carcinoma

“…A previous report showed that LPCAT1 could facilitate OSCC cell invasiveness and proliferation via increasing the biosynthesis of platelet‐activating factors 37 . In addition, HOXA‐AS3 promoted OSCC cell proliferation via LPCAT1 38 . Our data showed an overt elevation in LPCAT1 expression in OSCC samples and cells.…”

“…37 In addition, HOXA-AS3 promoted OSCC cell proliferation via LPCAT1. 38 Our data showed an overt elevation in LPCAT1 expression in OSCC samples and cells.…”

Long non-coding RNA metastasis-related lung adenocarcinoma transcript 1 (MALAT1) forms a negative feedback loop with long non-coding RNA colorectal neoplasia differentially expressed (CRNDE) in sepsis to regulate lung cell apoptosis

“…Although many studies have shown the involvement of lncRNAs in sepsis [ 19 , 20 ], the roles of most lncRNAs in this disease remain unclear and need to be further explored.…”

The integrated comprehension of lncRNA HOXA-AS3 implication on human diseases

“…Recent evidence showed that the overexpression of HOXA-AS3 was revealed in various types of human diseases and pathophysiological processes, including liver cancer [23][24][25], glioma [26,27], lung cancer [28][29][30], oral cancer [31], colorectal cancer [32], gastric cancer [33], pancreatic cancer [34], endometriosis [35], atherosclerosis [36,37], pulmonary arterial hypertension [38], and the lineage differentiation of mesenchymal stem cells (MSCs) [39]. In addition, high HOXA-AS3 expression has been identified to correlate with unfavorable clinicopathological features and poor prognoses, such as pathological grade, TNM stage, tumor size, lymph node metastasis, invasion depth, and Helicobacter pylori infection status, overall survival, and disease-free survival (Table 1).…”

“…lncRNA HOXA Cluster Antisense RNA 3 (HOXA-AS3) is a newly discovered lncRNA with 25,952 bases and in the genomic location at human chromosome 7p15.2 started 900 nt downstream of the 3' end of HOXB5 [22]. HOXA-AS3 expression has been implicated in a variety of human diseases and pathophysiological processes, including liver cancer [23][24][25], glioma [26,27], lung cancer [28][29][30], oral cancer [31], colorectal cancer [32], gastric cancer [33], pancreatic cancer [34], endometriosis [35], atherosclerosis [36,37], pulmonary arterial hypertension [38,39], and even the lineage differentiation of mesenchymal stem cells (MSCs) [39], according to numerous studies. In these disorders, high HOXA-AS3 expression has been reported to closely relate to several clinicopathologic characteristics, such as pathological grade, TNM stage, tumor size, lymph node metastasis, invasion depth, and Helicobacter pylori infection status, overall survival, and disease-free survival.…”