Long non-coding RNA LINC00511 regulates the expression of microRNA-625-5p and activates signal transducers and activators of transcription 3 (STAT3) to accelerate the progression of gastric cancer

Abstract

This study aimed to investigate the expression, biological function, and downstream mechanism of LINC00511 in gastric cancer (GC). In paired GC samples, LINC00511, miR-625-5p and STAT3 mRNA expression levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR); STAT3 protein expression was detected by immunohistochemical (IHC). The gain-of-function and loss-of-function models were established, and the proliferative and migrative ability of GC cells were measured by CCK-8 and transwell assays, respectively. The regulatory relationship between miR-625-5p and LINC00511 or STAT3 was examined by bioinformatics analysis, luciferase reporter gene assay, qRT-PCR, and western blot. We reported that LINC00511 and STAT3 expressions in GC tissues and cell lines were observably up-regulated, while miR-625-5p expression was inhibited. High expression of LINC00511 could facilitate the proliferation and promote the migration of GC cells. miR-625-5p was proved to be a downstream target of LINC00511, and LINC00511 could induce the expression of STAT3 by inhibiting the expression of miR-625-5p. Additionally, knockdown of LINC00511 suppressed the growth and lung metastases of CRC cells in nude mice. In conclusion, LINC00511 promotes the GC cell proliferation and migration via targeting the miR-625-5p/STAT3 axis, implying that LINC00511 can function as a target for GC therapy.

Long non-coding RNA FGD5-AS1 contributes to cisplatin resistance in hepatocellular carcinoma via sponging microRNA-153-3p by upregulating Twinfilin Actin Binding Protein 1 (TWF1)

“…Subsequently, membranes were incubated with secondary antibody (Abcam). Protein bands were visualized by an enhanced chemiluminescent kit (Applygen) [ 22 ].…”

Long non-coding RNAs: Biogenesis, functions, and clinical significance in gastric cancer

“… 77 , 78 , 79 In addition, some lncRNAs, such as HOXD-AS1, TRPM2-AS, and LINC00691, have been reported to regulate the expression of key components in the STAT3 signaling pathway. 80 , 104 , 105 …”

Molecular Classification of Breast Cancer Utilizing Long Non-Coding RNA (lncRNA) Transcriptomes Identifies Novel Diagnostic lncRNA Panel for Triple-Negative Breast Cancer

“…In gastric cancer (GC), LINC00511 was highly expressed and further studies found LINC00511 to recruit EZH2 (enhancer of zeste 2 polycomb repressive complex 2 subunit) to the PTEN (phosphatase and tensin homolog) promoter, facilitating its methylation, and subsequently activating the PI3K/AKT pathway, promoting GC cell proliferation, migration, and stemness, while inhibiting GC cell apoptosis [ 32 ]. LINC00511 was also found to regulate the expression of microRNA-625-5p and activate signal transducers and activators of transcription 3 (STAT3) to accelerate the progression of GC [ 33 ].…”

“…In colorectal cancer (CRC), the overexpression of LINC00511 accelerated CRC development by facilitating cell proliferation, metastasis, and stemness. Studies found that LINC00511 acted as a competing endogenous RNA (ceRNA) with NFIA (Nuclear Factor I A) to bind with miR-29c-3p, revealing the LINC00511/miR-29c-3p/NFIA axis as potential therapeutic targets for CRC treatment [ 33 , 34 ]. In a study on temozolomide (TMZ), the first-line chemotherapy drug for glioblastoma (GBM), LINC00511 expression upregulation correlated with the poor prognosis of GBM patients and LINC00511 silencing impaired the tolerance of TMZ, while its overexpression increased the TMZ resistance of sensitive GBM cells [ 35 ].…”