Long non-coding RNA (lncRNA): A potential therapeutic target in acute lung injury

Zaki, Almaz; Ali, Mohammad Shadab; Hadda, Vijay; Ali, Syed Mansoor; Chopra, Anita; Fatma, Tasneem

doi:10.1016/j.gendis.2021.07.004

Cited by 21 publications

(14 citation statements)

References 101 publications

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“…Long noncoding RNAs (lncRNAs), one kind of noncoding RNA having a length of more than 200 nucleotides, are an important topic in lung inflammation and damage 6 . Emerging evidence has reported that lncRNAs perform various functions in a series of biological processes in illnesses, and lncRNAs play key roles in diagnosing and managing sepsis 7 .…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA MALAT1 inhibition attenuates sepsis‐induced acute lung injury through modulating the miR‐129‐5p/PAX6/ZEB2 axis

Liu

Xiao

Lin

2023

Microbiology and Immunology

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This research aimed to investigate the role of the long noncoding RNA metastasisassociated lung adenocarcinoma transcript 1 (MALAT1)/microRNA-129-5p (miR-129-5p)/paired box gene 6 (PAX6) axis in sepsis-induced acute lung injury (ALI). MLE-12 cells and C57BL/6 mice were induced by LPS to establish lung injury in in vitro and in vivo models. Cell viability and apoptosis were measured by cell counting kit-8 assay and TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, respectively. Levels of inflammatory cytokines in cell supernatants and bronchoalveolar lavage fluid (BALF) were detected by ELISA. Lung injury was evaluated by lung wet weight-to-dry weight ratio and hematoxylin-eosin staining. MALAT1, PAX6, and zinc finger E-box-binding homeobox 2 (ZEB2) expression was elevated and miR-129-5p expression was reduced in the serum of patients with sepsis-induced ALI, LPS-induced MLE-12 cells, and lung tissues of ALI mice. MALAT1 interference delayed the LPS-induced cell proliferation decrease, apoptosis increase, and inflammatory factor increase. miR-129-5p inhibition could reverse the delaying effect of MALAT1 interference on LPS-induced lung cell injury. PAX6 overexpression (oe) reversed the inhibitory effect of miR-129-5p oe on LPSinduced lung cell injury. Downregulating MALAT1 reduced pulmonary edema, inflammatory cytokine levels, lung injury, and apoptosis in ALI mice. Moreover, miR-129-5p suppression or PAX6 oe reversed the delaying effect of MALAT1 interference on sepsis-induced ALI. MALAT1 aggravates sepsis-induced ALI via the miR-129-5p/PAX6/ZEB2 axis.

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Section: Introductionmentioning

confidence: 99%

Long noncoding RNA MALAT1 inhibition attenuates sepsis‐induced acute lung injury through modulating the miR‐129‐5p/PAX6/ZEB2 axis

Liu

Xiao

Lin

2023

Microbiology and Immunology

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“…Another major class of non-coding RNA is lncRNA which are more than 200 nucleotides in length and is the second most widely studied ncRNA in human diseases after miRNA. Multiple studies have shown the dysregulation of lncRNA in brotic diseases, including pulmonary brosis as well as in acute lung injury [81], [82].…”

Section: Lncrnamentioning

confidence: 99%

“…MALAT1 is a prominent lncRNA involved in many diseases including acute lung injury [82]. MALAT1 has been reported in macrophage activation and associated with pulmonary brosis.…”

Section: Lncrnamentioning

confidence: 99%

Non-Coding RNA in Idiopathic Interstitial Pneumonia and Covid-19 Pulmonary Fibrosis

Ali

Singh

Alam

et al. 2022

Preprint

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Pulmonary fibrosis is the major manifestation of idiopathic interstitial pneumonia as well as post-COVID-19 complications. The pathogenesis of PF is a complex molecular process that involves many types of cells, proteins, genes, and regulatory elements. The non-coding RNA is the main regulatory element in this process which mainly includes; miRNA, circRNA, and lncRNA. These regulatory elements control the expression of many important genes and various pathways that are involved in the pathogenesis of pulmonary fibrosis. Identification and molecular mechanisms by which these non-coding RNA molecules works are very important because they do not only help to understand the molecular basis of the disease but could also serve as a potential diagnostic/prognostic marker as well as therapeutic targets. In this review, we have provided the latest findings and discussed the role of these regulatory elements in various biological processes and pathways involved in the pathogenesis of pulmonary fibrosis associated with IIPs and Covid-19.

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“…The lncRNAs may also take part in chromatin modification [ 36 ]. Many lncRNAs have been implicated in pathophysiological processes such as cancer [ 37 ], inflammation [ 38 ], hematopoiesis [ 31 ], and metabolism [ 39 ]; the interest in lncRNAs has grown with the advancement of high-throughput technology.…”

Section: Exosome-specific Ncrnasmentioning

confidence: 99%

Fighting Fire with Fire: Exosomes and Acute Pancreatitis-Associated Acute Lung Injury

et al. 2022

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Acute pancreatitis (AP) is a prevalent clinical condition of the digestive system, with a growing frequency each year. Approximately 20% of patients suffer from severe acute pancreatitis (SAP) with local consequences and multi-organ failure, putting a significant strain on patients’ health insurance. According to reports, the lungs are particularly susceptible to SAP. Acute respiratory distress syndrome, a severe type of acute lung injury (ALI), is the primary cause of mortality among AP patients. Controlling the mortality associated with SAP requires an understanding of the etiology of AP-associated ALI, the discovery of biomarkers for the early detection of ALI, and the identification of potentially effective drug treatments. Exosomes are a class of extracellular vesicles with a diameter of 30–150 nm that are actively released into tissue fluids to mediate biological functions. Exosomes are laden with bioactive cargo, such as lipids, proteins, DNA, and RNA. During the initial stages of AP, acinar cell-derived exosomes suppress forkhead box protein O1 expression, resulting in M1 macrophage polarization. Similarly, macrophage-derived exosomes activate inflammatory pathways within endothelium or epithelial cells, promoting an inflammatory cascade response. On the other hand, a part of exosome cargo performs tissue repair and anti-inflammatory actions and inhibits the cytokine storm during AP. Other reviews have detailed the function of exosomes in the development of AP, chronic pancreatitis, and autoimmune pancreatitis. The discoveries involving exosomes at the intersection of AP and acute lung injury (ALI) are reviewed here. Furthermore, we discuss the therapeutic potential of exosomes in AP and associated ALI. With the continuous improvement of technological tools, the research on exosomes has gradually shifted from basic to clinical applications. Several exosome-specific non-coding RNAs and proteins can be used as novel molecular markers to assist in the diagnosis and prognosis of AP and associated ALI.

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Long non-coding RNA (lncRNA): A potential therapeutic target in acute lung injury

Cited by 21 publications

References 101 publications

Long noncoding RNA MALAT1 inhibition attenuates sepsis‐induced acute lung injury through modulating the miR‐129‐5p/PAX6/ZEB2 axis

Long noncoding RNA MALAT1 inhibition attenuates sepsis‐induced acute lung injury through modulating the miR‐129‐5p/PAX6/ZEB2 axis

Non-Coding RNA in Idiopathic Interstitial Pneumonia and Covid-19 Pulmonary Fibrosis

Fighting Fire with Fire: Exosomes and Acute Pancreatitis-Associated Acute Lung Injury

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