Long non-coding RNA LSINCT5 promotes ovarian cancer cell proliferation, migration and invasion by disrupting the CXCL12/CXCR4 signalling axis

Abstract

Long stress-induced noncoding transcript 5 (LSINCT5) is a member of the LSINCT family, members of which are expressed during stress-induced cell formation and have also been reported to promote cancer progression. In the present study, the association between LSINCT5 expression and clinical significance was investigated and the biological function of LSINCT5 in epithelial ovarian cancer (EOC) was explored. LSINCT5 expression was examined in EOC tissues by reverse transcription-quantitative polymerase chain reaction and its association with clinicopathological factors was analysed. Cell proliferation, migration and invasion tests were performed to observe the role of LSINCT5 in human ovarian cancer cell lines in vitro. The negative control (NC) and siLSINCT5 SKOV3 cells were treated with chemokine ligand 12 (CXCL12) and their proliferation, migration and invasion activities were examined. LSINCT5 was overexpressed in EOC compared with normal ovarian tissue. LSINCT5 expression was significantly associated with the International Federation of Gynecologists and Obstetricians cancer stage and the presence of lymphatic metastases. Silencing LSINCT5 significantly reduced the expression of chemokine receptor 4 (CXCR4) and inhibited SKOV3 cell proliferation, migration and invasion, however the CXCL12 expression level had no significant change. When NC and siLSINCT5-SKOV3 cells were treated with CXCL12, the proliferation and invasion ability were significantly enhanced. The migration ability of the siLSINCT5-SKOV3 cells was also significantly enhanced. The present study indicated that LSINCT5 serves an important role in ovarian cancer metastasis by regulating the CXCL12/CXCR4 signalling axis, suggesting that this pathway may be a potential target for the treatment of patients with EOC.

Determination of Potential Therapeutic Targets and Prognostic Markers of Ovarian Cancer by Bioinformatics Analysis

“…The expression of CXCR6 in epithelial ovarian cancer is significantly higher than that in normal ovarian and benign tissues. Studies have shown that the expression of CXCR6 is associated with lymph node metastasis [36], and the same conclusions have been obtained in cell experiments [45]. Ovarian cancer cell lines OVCAR-3 and SKOV-3 with high expression of CXCR6 have higher migration and invasion abilities.…”

<p>lncRNA LSINCT5 Regulates miR-20a-5p/XIAP to Inhibit the Growth and Metastasis of Osteosarcoma Cells</p>

“… 19 As a newly discovered lncRNA in recent years, LSINCT5 is located on the human 5p15.33 chromosome. Previous research has shown that LSINCT5 affects the development and progression of colorectal carcinoma, ovarian cancer, 20 gastric cancer, 21 esophageal cancer, and chronic heart failure, 22 , 23 but there is relatively little research on OS at present. However, our study revealed that LSINCT5 was highly expressed in OS tissues and cell strains, and the 5-year survival of patients with high expression decreased, which was consistent with the study of He et al 12 However, the relevant mechanism is still unclear.…”

LSINCT5 predicts unfavorable prognosis and exerts oncogenic function in osteosarcoma

“…Meanwhile, Xu et al [16] also showed high LSINCT5 expression was associated with large tumor size, deep tumor invasion depth and advanced TNM stage in colorectal cancer patients. Long et al [18] demonstrated that ovarian cancer patients with high LSINCT5 expression tended to have advanced FIGO stage and lymph node metastasis. However, Mansoori et al [19] indicated there was no significant correlation between LSINCT5 expression and clinicopathological characteristics in breast cancer patients.…”