Long non-coding RNA MEG3 induces cell apoptosis in esophageal cancer through endoplasmic reticulum stress

Huang, Zhen-Lun; Chen, Rui-Pei; Zhou, Xiao‐Tao; Zhan, Hao-Lian; Mei, Hu; Liu, Bin; Wu, Guandi; Wu, Ling‐Fei

doi:10.3892/or.2017.5568

Cited by 50 publications

(51 citation statements)

References 25 publications

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“…For instance, upregulation of MEG3 inhibited breast cancer cells proliferation and invasion capacities . MEG3 was able to inhibit cell growth and induced apoptosis in esophageal squamous cell carcinoma EC109 cells . However, to our knowledge, there is only one literature reported the functional impacts of MEG3 on OS cells, and in which MEG3 was indicated as a tumor suppressor .…”

Section: Discussionmentioning

confidence: 99%

Retracted: Knockdown of lncRNA MEG3 inhibits viability, migration, and invasion and promotes apoptosis by sponging miR‐127 in osteosarcoma cell

Wang

Kong

2017

J of Cellular Biochemistry

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Osteosarcoma (OS) is one of the most common bone malignancies and occurs almost exclusively in children and adolescents. This study aimed to explore the role of lncRNA maternally expressed gene 3 (MEG3) in OS cells growth and metastasis, and to uncover the possible underlying mechanism. In this study, the expressions of MEG3 in five OS cell lines (MG63, OS-732, SaOS, G292, and 143B) and in a human osteoblast cell line hFOB1.19 were measured by qRT-PCR analysis. The expressions of MEG3, miR-127, and ZEB1 in OS-732 cells were overexpressed or suppressed by transfection. Cell viability, migration, invasion, and apoptosis were then assessed.The results showed that MEG3 was highly expressed in OS cell lines when compared to hFOB1.19 cell. MEG3 silence significantly suppressed OS-732 cells growth and metastasis, as evidenced by the decreases in cell viability, migration, invasion, and increase in apoptotic cell rate. MEG3 acted as an endogenous sponge by binding to miR-127. More interestingly, MEG3 silence could not suppress OS-732 cells growth and metastasis when miR-127 was knocked down. ZEB1 was a target gene of miR-127, and miR-127 overexpression-induced impairments in cell growth and metastasis were attenuated when ZEB1 was overexpressed. Moreover,miR-127 suppression activated JNK and Wnt signaling pathways, while these activations were recovered by ZEB1 silence. To conclude, our findings suggest that lncRNA MEG3 promoted OS cells growth and metastasis in vitro through sponging miR-127. This study provides the evidence that MEG3 may be a potential therapeutic target for OS. K E Y W O R D SMEG3, miR-127, osteosarcoma, ZEB1

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Section: Discussionmentioning

confidence: 99%

Retracted: Knockdown of lncRNA MEG3 inhibits viability, migration, and invasion and promotes apoptosis by sponging miR‐127 in osteosarcoma cell

Wang

Kong

2017

J of Cellular Biochemistry

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“…It was previously determined that ERS was prevalent in tumor tissues (25). ERS was positively correlated with the depth of tumor invasion and degree of metastasis, indicating that ERS might be an important inducement of invasion and metastasis of tumor cells (26). ERS, a type of self-protective reaction of the cells, is involved in the occurrence and development of breast, liver and pancreatic cancer (27)(28)(29).…”

Section: Discussionmentioning

confidence: 99%

Expression of ERO1L in gastric cancer and its association with patient prognosis

Zhou

Wang

Gao

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. The present study aimed to assess the expression of endoplasmic reticulum oxidoreductin-1-like (ERO1L) in gastric cancer and determine its association with patient prognosis. A total of 105 patients with gastric cancer undergoing radical gastrectomy were selected for the current study. Gastric cancer tissues (the observation group) and normal gastric tissue adjacent to the carcinoma (the control group) were resected from patients. Levels of ERO1L mRNA and protein in tumor tissues and adjacent tissues were detected using reverse transcription-quantitative polymerase chain reaction, western blotting and immunohistochemistry. Patients were divided into two groups: A positive group and negative group, according to the expression of ERO1. The expression of ERO1L in gastric cancer and its association with patient prognosis was analyzed. Levels of ERO1 mRNA and protein in gastric cancer were significantly higher than those of adjacent tissues (P<0.05). Immunohistochemical analysis demonstrated that there were 22 patients exhibiting negative expression of ERO1L and 83 patients exhibiting positive expression of ERO1L. The cumulative recurrence rates over 3 years in patients with positive expression of ERO1L were significantly higher than in patients with negative expression of ERO1L (P<0.05); the cumulative survival rates over 3 years in patients with positive expression of ERO1L were significantly lower than those of patients with negative expression of ERO1L (P<0.05). Thus, the current study determined that ERO1L was highly expressed in gastric cancer tissue. The high expression of ERO1L was associated with adverse prognoses in patients with gastric cancer. ERO1L may therefore be a therapeutic target for the prevention of gastric cancer.

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“…36 Moreover, it has been shown that MEG3 inhibits cell growth and induces apoptosis in ESCC cells, mostly via activation of the endoplasmic reticulum stress pathway. 50 Furthermore, Zhang et al reported that MEG3 suppresses BC cell growth, invasion, and tumor angiogenesis via downregulation of AKT signaling pathway, and hence may serve as a novel therapeutic target of BC. 52 Consistent with these findings, Braconi and colleagues have reported that aberrant expression of MEG3 may F I G U R E 3 A, Forest plot demonstrating subgroup analysis of the relationship between MEG3 expression with OS in patients with GI; or B, breast cancer.…”

Section: Discussionmentioning

confidence: 99%

The prognostic value of long noncoding RNA MEG3 expression in the survival of patients with cancer: A meta‐analysis

Binabaj

Bahrami

Bahreyni

et al. 2018

J of Cellular Biochemistry

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Long noncoding RNAs (lncRNAs) play an important role in carcinogenesis and cancer progression. lncRNA maternally expressed gene 3 (MEG3) is a tumor suppressor that is downregulated in several cancers. However, its prognostic value in human malignancies remains controversial. We have therefore undertaken a meta-analysis to explore the relationship between cancer survival and the expression of lncRNA MEG3. A systematic literature search identified 13 potentially eligible investigations comprising 1733 patients in nine different cancer types. In the pooled analysis, a low expression of MEG3 was associated with low overall survival (OS) in patients with cancer having a combined hazard ratio (HR) of 0.830 (HR = 0.83; 95% CI: 0.70-0.98; p = 0.03; random effect model). However, subgroup analysis according to cancer type revealed that MEG3 expression was not associated with better OS in gastrointestinal cancer (HR = 0.58, 95% CI = 0.33-1.03; p = 0.06), and patients with breast cancer (HR = 0.85, 95% CI: 0.12-5.88; p = 0.87). In conclusion, our results demonstrate that only in the pooled analysis, there was a significant relationship between MEG3 expression and cancer survival. Further investigation of other molecular biomarkers involved in tumorigenesis-related pathways is necessary.

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Long non-coding RNA MEG3 induces cell apoptosis in esophageal cancer through endoplasmic reticulum stress

Cited by 50 publications

References 25 publications

Retracted: Knockdown of lncRNA MEG3 inhibits viability, migration, and invasion and promotes apoptosis by sponging miR‐127 in osteosarcoma cell

Retracted: Knockdown of lncRNA MEG3 inhibits viability, migration, and invasion and promotes apoptosis by sponging miR‐127 in osteosarcoma cell

Expression of ERO1L in gastric cancer and its association with patient prognosis

The prognostic value of long noncoding RNA MEG3 expression in the survival of patients with cancer: A meta‐analysis

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