Long non-coding RNA NEAT1 deteriorates polycystic ovary syndrome progression via binding microRNA-324-3p to target bromodomain containing 3

Abstract

Objective: Long non-coding RNA (LncRNA) nuclear-enriched abundant transcript 1 (NEAT1) has been studied in polycystic ovary syndrome (PCOS), while the regulatory mechanism of NEAT1 on PCOS by regulating microRNA (miR)-324-3p remains extensive exploration. Our study aims to investigate the role of NEAT1, miR-324-3p and bromodomain containing 3 (BRD3) in PCOS.Methods: Firstly, the PCOS rat model was established, and then NEAT1, miR-324-3p and BRD3 levels were detected in PCOS rats. The decreased NEAT1 or augmented miR-324-3p were injected into the PCOS rats to determine the change of biochemical indices and pathology. Also, the rescue experiment was conducted. Thereafter, the binding relations among NEAT1, miR-324-3p and BRD3 were analyzed.Results: NEAT1 and BRD3 were enriched while miR-324-3p was decreased in PCOS rats. The reduction of NEAT1 or the elevation of miR-324-3p mitigated the obesity and metabolic disorders in PCOS rats. NEAT1 sponged miR-324-3p as a competing endogenous RNA and miR-324-3p targeted BRD3. The elevated miR-324-3p or reduced BRD3 reversed effects of enhanced NEAT1.Conclusion: NEAT1 exacerbates obesity and metabolic disorders of PCOS rats via regulating miR-324-3p to target BRD3. This study affords a novel direction for PCOS treatment.