Long non-coding RNA PCAT-1 promotes tumor progression by inhibiting miR-129-5p in human ovarian cancer

Gu, Ling; Jin, Shuangshuang; Xu, Rui‐hua; Zhang, Jing; Geng, Ying-Chun; Shao, Xingyue; Libo, Qin

doi:10.5114/aoms.2018.75534

Cited by 20 publications

(17 citation statements)

References 22 publications

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“…Another study has verified that intrathecal injection of miR-129-5p mimic reduced the levels of toll-like receptor 3, high-mobility group box-1, TNF-α and IL-1β [2]. Moreover, it was suggested up-regulation of miR-129-5p can efficiently inhibits proliferation and induces apoptosis of ovarian cancer cells [13]. The study also showed that down-regulating SOX6 descended the apoptosis and ascended the proliferation of neuronal cells of AD rats as well as alleviate the neuronal injury and inflammatory response in AD rats.…”

Section: Discussionmentioning

confidence: 90%

RETRACTED ARTICLE: MicroRNA-129-5p alleviates nerve injury and inflammatory response of Alzheimer’s disease via downregulating SOX6

et al. 2019

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There is growing evidence of the position of microRNAs (miRs) in Alzheimer's disease (AD), thus our objective was to discuss the impact of miR-129-5p regulating nerve injury and inflammatory response in AD rats by modulating SOX6 expression. The AD rat model was established by injecting Aβ 25-35 into the brain. The pathological changes, ultrastructure, number of neurons, cell degeneration and apoptosis of hippocampal tissue were observed in vivo. MiR-129-5p, SOX6, IL-1β, TNF-α, Bcl-2 and Bax expression in serum and hippocampal tissues were detected by ELISA, RT-qPCR or western blot analysis. The successfully modeled hippocampal neuronal cells of AD were transfected with miR-129-5p mimic, SOX6-siRNA or their controls to figure out their roles in proliferation, apoptosis and inflammatory reaction in vitro. Low expression of SOX6 and high expression of miR-129-5p in vivo of rats would shorten the escape latent period and increase the times of crossing platforms, alleviate the pathological injury, inhibit neuronal apoptosis and reduce the inflammatory reaction. Up-regulation of miR-129-5p and down-regulation of SOX6 promoted proliferation, suppressed apoptosis and degraded the inflammatory reaction of neuronal cells in vitro. Up-regulation of SOX6 reversed the expression of miR-129-5p to reduce the damage and inflammatory response of the cell model of AD. Our study presents that upregulation of miR-129-5p or down-regulation of SOX6 can reduce nerve injury and inflammatory response in rats with AD. Thus, miR-129-5p may be a potential candidate for the treatment of AD.

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Section: Discussionmentioning

confidence: 90%

RETRACTED ARTICLE: MicroRNA-129-5p alleviates nerve injury and inflammatory response of Alzheimer’s disease via downregulating SOX6

et al. 2019

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“…Upregulation of lncRNA PCAT-1 (prostate cancer-associated transcript-1) in OvCa cell lines and tissue samples and its ability to inhibit apoptosis and to promote proliferation, migration, and invasion of OvCa cells was shown in two studies ( Table 2 ). Oncogenic lncRNA PCAT-1 can bind suppressive miRNAs such as miR-129-5p and miR-124-3p, thus limiting their level in OvCa [ 159 , 160 ]. Moreover, direct interaction of PCAT-1 with miR-129-5p was validated using the luciferase reporter assay [ 159 ], and interaction with miR-124-3p was demonstrated via qRT-PCR, transfection, and loss/gain of function studies [ 160 ].…”

Section: Oncogenic Lncrnas As Cernas In Ovarian Cancermentioning

confidence: 99%

“…Oncogenic lncRNA PCAT-1 can bind suppressive miRNAs such as miR-129-5p and miR-124-3p, thus limiting their level in OvCa [ 159 , 160 ]. Moreover, direct interaction of PCAT-1 with miR-129-5p was validated using the luciferase reporter assay [ 159 ], and interaction with miR-124-3p was demonstrated via qRT-PCR, transfection, and loss/gain of function studies [ 160 ]. Some potential targets (e.g., cyclin D1, CDK6, p53, Bax, Wnt3a, β-catenin, etc.)…”

Section: Oncogenic Lncrnas As Cernas In Ovarian Cancermentioning

confidence: 99%

LncRNAs in Ovarian Cancer Progression, Metastasis, and Main Pathways: ceRNA and Alternative Mechanisms

Брага

Фридман

Moscovtsev

et al. 2020

IJMS

163

103

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Ovarian cancer (OvCa) develops asymptomatically until it reaches the advanced stages with metastasis, chemoresistance, and poor prognosis. Our review focuses on the analysis of regulatory long non-coding RNAs (lncRNAs) competing with protein-coding mRNAs for binding to miRNAs according to the model of competitive endogenous RNA (ceRNA) in OvCa. Analysis of publications showed that most lncRNAs acting as ceRNAs participate in OvCa progression: migration, invasion, epithelial-mesenchymal transition (EMT), and metastasis. More than 30 lncRNAs turned out to be predictors of survival and/or response to therapy in patients with OvCa. For a number of oncogenic (CCAT1, HOTAIR, NEAT1, and TUG1 among others) and some suppressive lncRNAs, several lncRNA/miRNA/mRNA axes were identified, which revealed various functions for each of them. Our review also considers examples of alternative mechanisms of actions for lncRNAs besides being ceRNAs, including binding directly to mRNA or protein, and some of them (DANCR, GAS5, MALAT1, and UCA1 among others) act by both mechanisms depending on the target protein. A systematic analysis based on the data from literature and Panther or KEGG (Kyoto Encyclopedia of Genes and Genomes) databases showed that a significant part of lncRNAs affects the key pathways involved in OvCa metastasis, EMT, and chemoresistance.

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“…The most common regulatory mechanism of lncRNAs is to function as competitive endogenous RNAs (ceRNAs), in which lncRNAs bind to targeted miRNA, resulting in downstream gene silencing [11]. miR-129-5p has recently been identified as a tumor suppressor in many types of cancer, including ovarian cancer [12], breast cancer [13], and osteosarcoma [14]. However, its biological role in LSCC has not been explored.…”

Section: Introductionmentioning

confidence: 99%

Small Nucleolar RNA Host Gene 12 (SNHG12) Promotes Proliferation and Invasion of Laryngeal Cancer Cells via Sponging miR-129-5p and Potentiating WW Domain-Containing E3 Ubiquitin Protein Ligase 1 (WWP1) Expression

Sun

Chen

et al. 2019

Med Sci Monit

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Background The clinical significance and biological function of long noncoding RNA SNHG12 have not been identified in laryngeal squamous cell carcinoma (LSCC). Material/Methods Expression levels of SNHG12, miR-129-5p, and WWP1 in LSCC tissues or cells were tested by RT-qPCR. MTT assay, flow cytometry, and Transwell assay were used to identify the progression of LSCC cells in vitro . Luciferase reporter assay was used to assess the associations among SNHG12, WWP1, and miR-129-5p. Results SNHG12 was significantly overexpressed in LSCC tissues compared with adjacent normal tissues. The expression level of SNHG12 was significantly associated with T classification, lymph node metastasis, and cancer stage of LSCC. High expression of SNHG12 predicted shorter disease-free survival. Suppressing SNHG12 using siRNA inhibited proliferation and invasion and promoted apoptosis in the AMC-HN-8 LSCC cell line. SNHG12, mainly located in cytoplasm of AMC-HN-8 cells, was validated by dual luciferase reporter test and RT-qPCR to directly interact with miR-129-5p. Inhibition of miR-129-5p significantly increased proliferation and invasion of AMC-HN-8 cells and ameliorated the suppressive effects of si-SNHG12. Luciferase assay showed that miR-129-5p was able to combine with the 3′UTR region of WWP1, which is generally regarded as an E3 ubiquitin protein ligase. RT-qPCR and Western blot showed that WWP1 was positively regulated by SNHG12 and negatively regulated by miR-129-5p at the mRNA level and protein level. Overexpression of WWP1 significantly increased proliferation and invasion of laryngeal cancer cells. Moreover, when SNHG12 was suppressed, rescue of WWP1 restored the proliferation and invasion abilities of AMC-HN-8 cells. Conclusions Our study demonstrated that SNHG12 promoted LSCC cells progression via sponging miR-129-5p and potentiating WWP1 expression.

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Long non-coding RNA PCAT-1 promotes tumor progression by inhibiting miR-129-5p in human ovarian cancer

Cited by 20 publications

References 22 publications

RETRACTED ARTICLE: MicroRNA-129-5p alleviates nerve injury and inflammatory response of Alzheimer’s disease via downregulating SOX6

RETRACTED ARTICLE: MicroRNA-129-5p alleviates nerve injury and inflammatory response of Alzheimer’s disease via downregulating SOX6

LncRNAs in Ovarian Cancer Progression, Metastasis, and Main Pathways: ceRNA and Alternative Mechanisms

Small Nucleolar RNA Host Gene 12 (SNHG12) Promotes Proliferation and Invasion of Laryngeal Cancer Cells via Sponging miR-129-5p and Potentiating WW Domain-Containing E3 Ubiquitin Protein Ligase 1 (WWP1) Expression

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