Long Non-Coding RNA Signatures Associated with Ferroptosis Predict Prognosis in Colorectal Cancer

Abstract

Background: Currently, colorectal cancer has become a common gastrointestinal malignancy that usually occurs in the colon and rectum, and ferroptosis plays a vital role in the pathology and progression of colorectal tumors. Methods: A total of 627 patients (51 normal and 644 tumor samples) from The Cancer Genome Atlas (TCGA)-COAD and TCGA-READ were included in the study. Lasso and Cox's regression was employed to analyze the characteristic lncRNAs in colorectal cancer samples, and a distinctive prognostic model of ferroptosis-related lncRNAs was established. By analyzing the divergence between the high and low-risk groups of ferroptosis-related lncRNAs, 15 characteristic lncRNAs related to the prognosis of colorectal cancer were evaluated. Kaplan-Meier analysis, operation characteristic curve (ROC), nomogram, and gene set enrichment analyses (GSEA) further confirmed the validity of the characteristic prognostic model with ferroptosis-related lncRNAs. Results: Kaplan-Meier analysis confirmed a high-risk group of ferroptosis-related lncRNA interrelated with a poor prognosis in colorectal cancer. AUC estimates of 1 -, 3 -, and 5-year survival rates for ferroptosis-related lncRNA characteristic models were 0.745, 0.767 and 0.789. GSEA analysis showed that immune and malignancy-related pathways were active in the high-risk score group. In addition, differential analyses of immune function, including Checkpoint, cytolytic, HLA, and T cell co-inhibition, differed significantly betwixt low -and high-risk groups.CD160,

Construction of immune-related lncRNA signature to predict aggressiveness, immune landscape, and drug resistance of colon cancer

“…7 , the level of LAG3 expression was higher in the high-risk group compared to the low-risk group, demonstrating the potential effect of LAG3 on shaping the tumor immune microenvironment. This finding is consistent with a recently published study demonstrating that the expression level of LAG3 in colorectal cancer is tightly associated with the levels of AC124067.4, AL137782.1 and AC010973.2 which are involved in our models [ 39 ]. These results indicate a potential functional link of those lncRNAs to LAG3 expression.…”

N7-methylguanosine-related lncRNAs: Predicting the prognosis and diagnosis of colorectal cancer in the cold and hot tumors

“…Among the 17 lncRNAs related to m7G identified in our study, only AL137782.1, AC012313.5, AL031985.3, AC007728.3 and AC099850.4 are protective factors, but the other lncRNAs were risk factors. Consistent with our results, Previous reports have shown that AC133540.1, AL137782.1, AP001619.1, AC013652.1, AC008760.1, ALMS1-IT1, AC012313.5, FGF14-AS2, AP006621.2, LINC00702, LINC02550 and AC083900.1 are associated with poor prognosis in CRC patients (Huang and Pan, 2019;Hou et al, 2020;Yu et al, 2020;Xu et al, 2021a;Li et al, 2021;Li et al, 2022), whereas AC007991.4, LINC02381, AL031985.3 and AC099850.4 were reported in the other cancer patients with poor prognosis (Jafarzadeh and Soltani, 2020;Zhang et al, 2020;Zhao et al, 2020;Wu et al, 2021). The other lncRNAs were initially discovered.…”

Identification of RNA Methylation-Related lncRNAs Signature for Predicting Hot and Cold Tumors and Prognosis in Colon Cancer

“…Previous studies have shown that upregulation of ALMS1-IT1 can promote lung cancer progression by mediating AVL9 activation of the cell cycle protein-dependent kinase pathway ( Luan et al, 2021 ). Li et al constructed a ferroptosis-related lncRNA prognostic signature that also included ALMS1-IT1 and found it to be strongly associated with poor prognosis in colon cancer ( Li et al, 2022 ). These findings validated the oncogenic properties of ALMS1-IT1 and are consistent with our results.…”