Long non-coding RNA small nucleolar RNA host gene 12 (SNHG12) promotes cell proliferation and migration by upregulating angiomotin gene expression in human osteosarcoma cells

Ruan, Wendong; Wang, Pei; Feng, Shiqing; Xue, Yuan; Li, Yulin

doi:10.1007/s13277-015-4256-7

Cited by 96 publications

(84 citation statements)

References 30 publications

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“…To explore the potential mechanism underlying the effects of SNHG12, an FCM assay determined that silencing SNHG12 expression promoted apoptosis and inhibited migration and invasion of cervical cancer cells. Our results confirmed that the overexpression of SNHG12 promoted cell proliferation and migration and inhibited apoptosis [16-18]. Our study expanded upon the current knowledge of the downstream regulatory mechanism of SNHG12.…”

Section: Discussionsupporting

confidence: 83%

“…Previous studies have found that SNHG12 expression was increased in and contribute to the metastasis of several types of cancer [16-18]. We assessed the effect of SNHG12 on cervical cancer cells, and we observed that the downregulation of SNHG12 led to a significant decrease in the migratory and invasive capacities of the cells (Fig.…”

Section: Resultsmentioning

confidence: 78%

“…The lncRNA nuclear enriched abundant transcript 1 (NEAT1) was found to be overexpressed and associated with tumor progression in prostate cancer [15]. Recent studies have demonstrated that the lncRNA small nucleolar RNA host gene 12 (SNHG12) was increased in several cancers, including osteosarcoma, breast cancer and hepatocellular carcinoma [16-18]. However, the expression and role of SNHG12 in cervical cancer remain unknown and warrant investigation.…”

Section: Introductionmentioning

confidence: 99%

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Upregulation of Long Non-Coding RNA Small Nucleolar RNA Host Gene 12 Contributes to Cell Growth and Invasion in Cervical Cancer by Acting as a Sponge for MiR-424-5p

Dong

Wang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Cervical cancer, which is one of the most aggressive cancers affecting females, has high rates of recurrence and mortality. Small nucleolar RNA host gene 12 (SNHG12) is known to promote the progression of several cancers; however, its exact effects and molecular mechanisms in cervical cancer remain unknown. Methods: Real-time quantitative PCR was used to determine the expression level of SNHG12 in cervical cancer tissues and cell lines. Loss-of-function assays were performed to examine the effect of SNHG12 on the proliferation, apoptosis, migration and invasion of cervical cancer cells in vitro and tumor growth in vivo. Luciferase experiments were employed to explore the interactions between SNHG12 and miR-424-5p. Results: SNHG12 was found to be abnormally elevated in human cervical cancer tissues compared with paired adjacent normal tissues. Moreover, high SNHG12 expression in tumor tissues was significantly correlated with vascular involvement, lymph node metastasis, advanced FIGO stage and poor prognosis. Furthermore, the knockdown of SNHG12 was found to inhibit proliferation, migration and invasion of cervical cancer cells in vitro, and silencing SNHG12 was shown to suppress tumor growth in a nude mouse model. Mechanistic studies showed that SNHG12 functioned as an endogenous sponge for miR-424-5p, thereby downregulating the expression of miR-424-5p in cervical cancer. Furthermore, the inhibition of miR-424-5p in SNHG12-depleted cells partially reversed the effects on cervical cancer cell apoptosis, adhesion and invasion. Conclusion: In summary, our findings suggest that the tumor-promoting role of SNHG12 is to function as a molecular sponge, which negatively regulates miR-424-5p. These findings may provide a potent therapeutic target for cervical cancer.

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Section: Discussionsupporting

confidence: 83%

Section: Resultsmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

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Upregulation of Long Non-Coding RNA Small Nucleolar RNA Host Gene 12 Contributes to Cell Growth and Invasion in Cervical Cancer by Acting as a Sponge for MiR-424-5p

Dong

Wang

et al. 2018

Cell Physiol Biochem

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“…57,58 Similarly, a recent study illuminated that the level of SNHG12 in CRC tissues is higher than the adjacent normal mucosa as detected by qRT-PCR. Furthermore, upregulation of SNHG12 was distinctly associated with larger tumor size, advanced tumor stage, and worse 5-year survival rate in CRC patients.…”

Section: Small Nucleolar Rna Host Gene 12mentioning

confidence: 93%

Long non-coding RNAs: New biomarkers for prognosis and diagnosis of colon cancer

Deng

Wang

et al. 2017

Tumour Biol.

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BackgroundColorectal cancer (CRC) is the third most diagnosed cancer in this blue planet, whose incidence is increasing in young adults, especially in the developing countries. 1 As the fourth main cause of cancer-related deaths in the world, CRC contributes to a serious threat to human health. To descend mortality of CRC, in past decades, researchers strive to study the mechanisms of this disease. Unfortunately, the prognosis of this malignancy remains pessimistic in numerous patients who even received surgery, and the cancer reappear or metastasis come up after several months. One of the leading challenges of this disease is the clinical diagnosis may be late. 2 Therefore, biological biomarkers for the identification of detection and prediction may have clinical significance. Especially, in past decade, extensive molecular research has demonstrated that long non-coding RNAs (lncRNAs) may be novel and significant biological biomarkers for early diagnosis and prognosis of colon cancer. 3 As its name indicates, LncRNAs are a group of noncoding RNA molecules whose lengths are larger than 200 nucleotides and lack protein-coding capability.

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“…High SPRY4-IT1 expression has been observed in renal cancer and down-regulation of SPRY4-IT1 reduces renal cancer cell proliferation, invasion and migration (10). EWSAT1 and SNHG12 were all found to be up-regulated in OS and act as tumor promoter (11,12).…”

Section: Introductionmentioning

confidence: 99%

MEG3 long non-coding RNA prevents cell growth and metastasis of osteosarcoma

Zhang¹,

Cai²,

Li³

2018

BLL

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OBJECTIVE: This study aimed to investigate the role of long non-coding RNA MEG3 (lncRNA MEG3) in osteosarcoma (OS) and further explore the underlying molecular mechanism. MATERIALS AND METHODS: The expression profi les of MEG3 in OS cell lines and normal osteoblast cell line were detected by qRT-PCR. MEG3 was over-expressed in OS cell line by using LV-MEG3. MTT and colonyformation assays were applied for cell proliferation analysis. Cell migration assay was applied to investigate the cell migration ability. In addition, the expression levels of cell growth and metastasis related factors (Notch1, Hes1, TGF-β, N-cadheren and E-cadheren) were determined to illustrate the mechanisms. RESULTS: We found that compared with normal osteoblast hFOB1.19 cell line, MEG3 was signifi cantly downregulated in MG63 and U2OS cell lines, particularly in MG-63 cells. MEG3 was signifi cantly up-regulated in MG63 cells by LV-MEG3. Cell proliferation and migration ability were obviously repressed by MEG3 over-expression. In addition, MEG3 over-expression markedly inhibited Notch1, Hes1,TGF-β and N-cadheren expression, and the expression level of E-cadheren was improved. CONCLUSIONS: These results indicated that MEG3 could prevent cell growth and metastasis of OS by repressing Notch and TGF-β signaling pathway, thus providing a potential therapeutic target for OS treatment (Tab. 1, Fig. 4, Ref. 30). Text in PDF www.elis.sk.

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Long non-coding RNA small nucleolar RNA host gene 12 (SNHG12) promotes cell proliferation and migration by upregulating angiomotin gene expression in human osteosarcoma cells

Cited by 96 publications

References 30 publications

Upregulation of Long Non-Coding RNA Small Nucleolar RNA Host Gene 12 Contributes to Cell Growth and Invasion in Cervical Cancer by Acting as a Sponge for MiR-424-5p

Upregulation of Long Non-Coding RNA Small Nucleolar RNA Host Gene 12 Contributes to Cell Growth and Invasion in Cervical Cancer by Acting as a Sponge for MiR-424-5p

Long non-coding RNAs: New biomarkers for prognosis and diagnosis of colon cancer

MEG3 long non-coding RNA prevents cell growth and metastasis of osteosarcoma

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