Long non-coding RNA SNHG17 is an unfavourable prognostic factor and promotes cell proliferation by epigenetically silencing P57 in colorectal cancer

Abstract

Recently, substantial evidence has demonstrated that long non-coding RNAs (lncRNAs) play critical roles in multiple cancers including colorectal cancer (CRC). Utilizing publicly available lncRNA-expression-profiling data from the Gene Expression Omnibus (GEO) dataset GSE21510, we screened SNHG17 as a new candidate lncRNA associated with CRC development and progression. We further demonstrated that SNHG17 was upregulated in CRC tissues, and that its overexpression was significantly correlated with tumor size, TNM stage, and lymph node metastasis in CRC patients. Moreover, SNHG17 knockdown significantly inhibited the proliferation of CRC cells, and induced cell cycle G1/G0 phase arrest and cell apoptosis. Consistent with these findings, SNHG17 silencing inhibited tumor growth in vivo. Mechanistic studies revealed the capability of lncRNA SNHG17 to epigenetically suppress P57 by binding to enhancer of zeste homolog 2 (a key component of polycomb repressive complex 2) in CRC cells, and quantitative real-time polymerase chain reaction assays demonstrated that SNHG17 expression levels were inversely correlated with those of P57 in CRC tissues. Furthermore, rescue experiments confirmed that SNHG17 exerted oncogenic functions partly through regulating P57 expression. These findings represent the first reporting of the roles and mechanisms associated with SNHG17 in CRC progression, highlighting SNHG17 as a potential therapeutic target for CRC patients.

Long noncoding RNA MAPKAPK5‐AS1 promotes colorectal cancer proliferation by partly silencing p21 expression

“…A large number of published works have reported that these lncRNAs play an important role in multiple tumors . Our previous research shows that lncRNA SNHG17 can promote the proliferation of CRC cells by inhibiting the expression of p57 . The high expression of lncRNA LL22NC03‐N64E9.1 could predict poor prognosis in patients with colon cancer .…”

lncRNA DLEU1 contributes to tumorigenesis and development of endometrial carcinoma by targeting mTOR

“…There are also many types of lncRNAs that have been studied. Growing evidence suggests that lncRNAs are closely related with tumor . For example, lncRNA H19 promotes breast cancer progression, and plasma circulating lncRNA H19 can also serve as a screening and diagnostic marker for early breast cancer .…”

Long Non-Coding RNA SH3PXD2A-AS1 Promotes Cell Progression Partly Through Epigenetic Silencing P57 and KLF2 in Colorectal Cancer

“…Our previous studies investigated the expression patterns of P57 in CRC tissues, and highlighted P57 as an important anti-oncogene in CRC progression [47, 48]. We also reported that lncRNA HOXA-AS2 mediated CRC proliferation partly through inhibiting P21 and KLF2 expression [30].…”

“…P57 is a crucial component of CKIs family, which also includes P15, P16, P21, and P27 [47]. Our previous studies investigated the expression patterns of P57 in CRC tissues, and highlighted P57 as an important anti-oncogene in CRC progression [47, 48].…”

“…Our previous studies have highlighted P21 and P57 as important anti-oncogenes in CRC progression [47, 48]. P15 and P16 have also been identified as tumor-suppressive proteins in CRC [49, 51].…”