Long non-coding RNA SPRY4-IT1 promotes cell proliferation and invasion by regulation of Cdc20 in pancreatic cancer cells

Guo, Wenhao; Zhong, Kunhong; Heng, Wei; Nie, Chao; Zhu, Yong‐Guan

doi:10.1371/journal.pone.0193483

Cited by 22 publications

(19 citation statements)

References 36 publications

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“…CDC20 regulates cyclins and interacts with various proteins. Guo et al found that CDC20 is regulated by lncRNA SPRY4-IT1 to induce cell growing and invasion in pancreatic tumour [30]. Wang et al reported that CDC20 exhibits cancer-promoting effects.…”

Section: Discussionmentioning

confidence: 99%

CDK1 and CCNB1 as potential diagnostic markers of rhabdomyosarcoma: validation following bioinformatics analysis

Zhang

Jiang

et al. 2019

BMC Med Genomics

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BackgroundRhabdomyosarcoma (RMS), a common soft-tissue malignancy in pediatrics, presents high invasiveness and mortality. However, besides known changes in the PAX3/7-FOXO1 fusion gene in alveolar RMS, the molecular mechanisms of the disease remain incompletely understood. The purpose of the study is to recognize potential biomarkers related with RMS and analyse their molecular mechanism, diagnosis and prognostic significance.MethodsThe Gene Expression Omnibus was used to search the RMS and normal striated muscle data sets. Differentially expressed genes (DEGs) were filtered using R software. The DAVID has become accustomed to performing functional annotations and pathway analysis on DEGs.The protein interaction was constructed and further processed by the STRING tool and Cytoscape software. Kaplan–Meier was used to estimate the effect of hub genes on the ending of sarcoma sufferers, and the expression of these genes in RMS was proved by real-time polymerase chain reaction (RT-PCR). Finally, the expression of CDK1 and CCNB1 in RMS was validated by immunohistochemistry (IHC).ResultsA total of 1932 DEGs were obtained, amongst which 1505 were up-regulated and 427were down-regulated. Up-regulated genes were largely enriched in the cell cycle, ECM-receptor interaction, PI3K/Akt and p53 pathways, whilst down-regulated genes were primarily enriched in the muscle contraction process. CDK1, CCNB1, CDC20, CCNB2, AURKB, MAD2L1, HIST2H2BE, CENPE, KIF2C and PCNA were identified as hub genes by Cytoscape analyses. Survival analysis showed that, except for HIST2H2BE, the other hub genes were highly expressed and related to poor prognosis in sarcoma. RT-PCR validation showed that CDK1, CCNB1, CDC20, CENPE and HIST2H2BE were significantly differential expression in RMS compared to the normal control. IHC revealed that the expression of CDK1 (28/32, 87.5%) and CCNB1 (26/32, 81.25%) were notably higher in RMS than normal controls (1/9, 11.1%; 0/9, 0%). Moreover, the CCNB1 was associated with the age and location of the patient’s onset.ConclusionsThese results show that these hub genes, especially CDK1 and CCNB1, may be potential diagnostic biomarkers for RMS and provide a new perspective for the pathogenesis of RMS.

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Section: Discussionmentioning

confidence: 99%

CDK1 and CCNB1 as potential diagnostic markers of rhabdomyosarcoma: validation following bioinformatics analysis

Zhang

Jiang

et al. 2019

BMC Med Genomics

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“…The levels of glucose uptake, lactate production, and cellular ATP levels were detected in SW480 cells upon the transfection of the indicated plasmids in SW480 cells. Results are presented as mean ± SEM, **p < 0.01. carcinoma (HCC) cells, pancreatic cancer cells, and melanoma cells (Khaitan et al 2011;Jing et al 2016;Guo et al 2018;Li et al 2019). Additionally, SPRY4-IT1 mediated the cell stemness of breast cancer cells in vitro, and correlated with the prognosis of breast cancer patients (Xiang et al 2019;Song et al 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, several studies indicated the critical role of SPRY4-IT1 in cancer progression and metastasis. SPRY4-IT1 promoted the proliferation and metastasis of hepatocellular carcinoma (HCC) cells, pancreatic cancer cells, and melanoma cells (Khaitan et al 2011 ; Jing et al 2016 ; Guo et al 2018 ; Li et al 2019 ). Additionally, SPRY4-IT1 mediated the cell stemness of breast cancer cells in vitro , and correlated with the prognosis of breast cancer patients (Xiang et al 2019 ; Song et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

SPRY4-IT1 promotes survival of colorectal cancer cells through regulating PDK1-mediated glycolysis

Liu

Huang

et al. 2020

Animal Cells and Systems

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Colorectal cancer (CRC) becomes the third leading cause of cancer-related deaths worldwide recently. The prognosis of CRC is still poor in decades, and targeted therapy is still a potential effective treatment. Long non-coding RNAs (lncRNAs) could regulate series of cellular functions and developmental processes. LncRNA-SPRY4-IT1 (GenBank ID AK024556) is derived from an intron of the SPRY4 gene, which was highly expressed in melanoma cells and affected the progression of multiple types of cancers. However, the mechanism of SPRY4-IT1 in CRC progression remains unclear. Herein, we found the high level of SPRY4-IT1 in human colorectal cancer (CRC) tissues and cells, and correlated with patients' prognosis. We further noticed that SPRY4-IT1 regulated CRC cell growth and glycolysis, and promoting PDK1 expression. Our data further confirmed that SPRY4-IT1 regulated CRC progression targeting PDK1. We therefore thought SPRY4-IT1 could serve as a promising molecular target for the treatment of CRC.

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“…SPRY4-Intronic transcript or Sprouty4-Intronic transcript 1 (SPRY4-IT1) is a 687 nt unspliced and polyadenylated transcript derived from the second intron of the SPRY4 gene, which is located at 5q31.3. It has recently been revealed as an oncogenic regulatory factor or tumor-suppressor in different types of human carcinomas, such as in melanoma, in which it is overexpressed [ 60 , 61 , 120 ]. Data indicate that SPRY4-IT1 is involved in cancer development through the regulation of alternative splicing or gene expression of its target genes.…”

Section: Lncrnas Dysregulated In Cancer Cervicalmentioning

confidence: 99%

Roles and Mechanisms of the Long Noncoding RNAs in Cervical Cancer

Durán

Ribeiro-dos-Santos

Vidal

2020

IJMS

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Cervical cancer (CC) continues to be one of the leading causes of death for women across the world. Although it has been determined that papillomavirus infection is one of the main causes of the etiology of the disease, genetic and epigenetic factors are also required for its progression. Among the epigenetic factors are included the long noncoding RNAs (lncRNAs), transcripts of more than 200 nucleotides (nt) that generally do not code for proteins and have been associated with diverse functions such as the regulation of transcription, translation, RNA metabolism, as well as stem cell maintenance and differentiation, cell autophagy and apoptosis. Recently, studies have begun to characterize the aberrant regulation of lncRNAs in CC cells and tissues, including Homeobox transcript antisense RNA (HOTAIR), H19, Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), Cervical Carcinoma High-Expressed 1 (CCHE1), Antisense noncoding RNA in the inhibitors of cyclin-dependent kinase 4 (ANRIL), Growth arrest special 5 (GAS5) and Plasmacytoma variant translocation 1 (PVT1). They have been associated with several disease-related processes such as cell growth, cell proliferation, cell survival, metastasis and invasion as well as therapeutic resistance, and are novel potential biomarkers for diagnosis and prognosis in CC. In this review, we summarize the current literature regarding the knowledge we have about the roles and mechanisms of the lncRNAs in cervical neoplasia.

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Long non-coding RNA SPRY4-IT1 promotes cell proliferation and invasion by regulation of Cdc20 in pancreatic cancer cells

Cited by 22 publications

References 36 publications

CDK1 and CCNB1 as potential diagnostic markers of rhabdomyosarcoma: validation following bioinformatics analysis

CDK1 and CCNB1 as potential diagnostic markers of rhabdomyosarcoma: validation following bioinformatics analysis

SPRY4-IT1 promotes survival of colorectal cancer cells through regulating PDK1-mediated glycolysis

Roles and Mechanisms of the Long Noncoding RNAs in Cervical Cancer

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