Long non‑coding RNA TUG1 knockdown repressed the viability, migration and differentiation of osteoblasts by sponging miR‑214

Abstract

The maxillofacial region in the human body is susceptible to fracture and corresponding soft tissue injury. In the current study, the effect of long non-coding RNA (lncRNA) taurine upregulated gene 1 (TUG1) on maxillofacial fracture development was investigated. In total, 50 patients diagnosed with maxillary fracture and 50 healthy volunteers were enrolled in this study. Participants' TUG1 expression level in serum was measured using reverse transcription-quantitative (RT-q)PCR. After transfection with small interfering (si)-TUG1, microRNA (miR)-214 mimic, miR-214 inhibitor, bone morphogenetic protein 2 (BMP2) mimic or a combination, the biological behavior of osteoblasts was evaluated using MTT, Transwell assays, RT-qPCR, flow cytometry and western blot analysis. Recovery experiments were used to explore the potential mechanism. Results demonstrated that TUG1 expression was decreased in the serum of patients with maxillary fractures. Knockdown of TUG1 repressed viability, migration and differentiation and induced apoptosis of osteoblasts. StarBase v2.0 revealed that TUG1 served as a sponge for miR-214 and BMP2 is a direct target of miR-214. Altogether, it was revealed that TUG1 expression was decreased in patients with maxillary fractures and TUG1 knockdown repressed the biological process of osteoblasts by sponging miR-214.

[Retracted] Downregulation of miR‐221‐3p by LncRNA TUG1 Promoting the Healing of Closed Tibial Fractures in Mice

“…For instance, LncRNA TUG1 can adsorb miR-29c-3p, which enhances the malignant behavior of gastric adenocarcinoma [5]. Yao et al have found that knockout of LncRNA TUG1 can inhibit viability, CM, and differentiation and lead to CA of osteoblasts in patients with maxillary fractures, suggesting that LncRNA TUG1 is also involved in the biological process of osteoblast CP and CA [6].…”

LncRNA TUG1 relieves renal mesangial cell injury by modulating the miR‐153‐3p/Bcl‐2 axis in lupus nephritis

“…Yao et al reported that lncRNA TUG1 knockdown repressed the viability, migration, and differentiation of osteoblasts by sponging miR‐214. 25 A study by Zhang et al 26 demonstrated that silencing lncRNA TUG1 inhibits acute myeloid leukemia cell viability and promotes apoptosis by targeting the microRNA‐221‐3p/KIT axis. We performed functional analysis of TUG1‐plasmid or miR‐153‐3p mimic to explore whether lncRNA TUG1 mediated the functions of LPS in HRMCs and conducted a rescue experiment.…”

“…A large number of studies have suggested that lncRNAs are involved in various biological processes, including cell proliferation, apoptosis, and invasion. Yao et al reported that lncRNA TUG1 knockdown repressed the viability, migration, and differentiation of osteoblasts by sponging miR‐214 25 . A study by Zhang et al 26 demonstrated that silencing lncRNA TUG1 inhibits acute myeloid leukemia cell viability and promotes apoptosis by targeting the microRNA‐221‐3p/KIT axis.…”

Functions of the bone morphogenetic protein signaling pathway through non-coding RNAs

“…Ultimately, they demonstrated that the lncRNA PVT1 acts as a miR-128-3p sponge and influences the regulation of the BMPs signaling pathway as well as the downstream signaling proteins BMP-2 and BMP-4 through the regulation of GREM1. More illustrative examples of the lncRNA/miRNA/mRNA axis functioning by activating BMPs signaling pathways are presented in Table 2 [ [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] , [57] , [58] ].…”