Long non-coding RNA ZFAS1 regulates cell proliferation and invasion in cervical cancer via the miR-190a-3p/KLF6 axis

Su, Yuehui; Hou, Wenjing; Zhang, Chunyan; Ji, Pengcheng; Hu, Rui; Zhang, Qiongying; Wang, Yao; Li, Panpan; Zhang, Huiping; Chen, Yueyue; Zhang, Xiaodong; Zhang, Mengzhen

doi:10.1080/21655979.2021.2022265

Cited by 15 publications

(15 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, Liu et al [49] found that lncRNA ZFAS1 promoted pancreatic adenocarcinoma metastasis via sponge aspiration of the miR-3924-mediated RHOA/ROCK2 pathway. The existing studies focus on the involvement of ZFAS1 in the multiplication and metastasis of cancer cells by regulating miRNA-mediated pathways [50,51], while herein, we reveal a novel role of ZFAS1 and the potential molecule mechanism in PC. We found that ZFAS1 can affect PC lipometabolism, but its underlying mechanism remains to be clarified.…”

Section: Discussionmentioning

confidence: 89%

lncRNA ZFAS1 Promotes HMGCR mRNA Stabilization via Binding U2AF2 to Modulate Pancreatic Carcinoma Lipometabolism

Wang

Ruan

et al. 2022

Journal of Immunology Research

View full text Add to dashboard Cite

Being one of the most lethal malignant tumors worldwide, pancreatic carcinoma (PC) shows strong invasiveness and high mortality. In tumorigenesis and progression, the role played by long-chain noncoding RNAs (lncRNAs) cannot be ignored. This article mainly probes into the function of lncRNA ZFAS1 in PC. ZFAS1 expression in PC and normal counterparts retrieved from the Genotype-Tissue Expression (GTEx) project and The Cancer Genome Atlas (TCGA) database was analysed by GEPIA2. Its expression profile in clinical specimens and human PC cell strains was quantified using qRT-PCR. Measurements of BxPC-3 cell multiplication and invasiveness employed CCK-8, plate clone formation test, and Transwell chamber assay. ZFAS1’s impact on lipid content in BxPC-3 cells was detected. RNA pulldown and RIP assays analyzed the interaction of ZFAS1 with U2AF2 and HMGCR in BxPC-3 cells. Finally, the impacts of U2AF2 and HMGCR on the biological behavior of BxPC-3 were observed. ZFAS1 was kept at a high level in PC tissues versus the normal counterparts. ZFAS1 gene knockout remarkably suppressed PC cell multiplication and invasiveness and decreased the contents of free fatty acids, total cholesterol, triglycerides, and phospholipids. Mechanistically, ZFAS1 stabilized HMGCR mRNA through U2AF2, thus increasing HMGCR expression and promoting PC lipid accumulation. Meanwhile, reduced PC cell viability and invasiveness were observed after downregulating U2AF2 and HMGCR. As an oncogene of PC, ZFAS1 can modulate lipometabolism and stabilize HMGCR mRNA expression by binding with U2AF2 in PC, which is a candidate target for PC diagnosis and treatment.

show abstract

Section: Discussionmentioning

confidence: 89%

lncRNA ZFAS1 Promotes HMGCR mRNA Stabilization via Binding U2AF2 to Modulate Pancreatic Carcinoma Lipometabolism

Wang

Ruan

et al. 2022

Journal of Immunology Research

View full text Add to dashboard Cite

show abstract

“…Recent research suggests that miR-190a-3p promotes the proliferation and migration of gliomas, opening up new possibilities for glioma treatment (Zhou et al, 2021). In another work, miR-190a-3p was downregulated by the lncRNA zinc finger antisense 1 in the mediation of cervical cancer cell proliferation and invasion (Su et al, 2022).…”

Section: Discussionmentioning

confidence: 98%

The targeting of DAPK1 with miR‐190a‐3p promotes autophagy in trophoblast cells

Luo,

Tian,

et al. 2024

Molecular Reproduction Devel

View full text Add to dashboard Cite

Pre‐eclampsia (PE) is a dangerous pathological status that occurs during pregnancy and is a leading reason for both maternal and fetal death. Autophagy is necessary for cellular survival in the face of environmental stress as well as cellular homeostasis and energy management. Aberrant microRNA (miRNA) expression is crucial in the pathophysiology of PE. Although studies have shown that miRNA (miR)‐190a‐3p function is tissue‐specific, the precise involvement of miR‐190a‐3p in PE has yet to be determined. We discovered that miR‐190a‐3p was significantly lower and death‐associated protein kinase 1 (DAPK1) was significantly higher in PE placental tissues compared to normal tissues, which is consistent with the results in cells. The luciferase analyses demonstrated the target‐regulatory relationship between miR‐190a‐3p and DAPK1. The inhibitory effect of miR‐190a‐3p on autophagy was reversed by co‐transfection of si‐DAPK1 and miR‐190a‐3p inhibitors. Thus, our data indicate that the hypoxia‐dependent miR‐190a‐3p/DAPK1 regulatory pathway is implicated in the development and progression of PE by promoting autophagy in trophoblast cells.

show abstract

“…As an important mechanism of lncRNA function, some lncRNAs with miRNA-binding sites can serve as 'sponges' to seclude endogenous miRNAs, which disturbs the modulation of gene expression mediated by miRNA. This phenomenon called competing endogenous RNAs (ceRNAs) belongs to the genome-wide finetuning regulatory molecules network [43][44][45]. PTEN pseudogene (PTENP1) is one of the ceRNAs correlated with PCa, which is linked to PTEN phosphatase as a tumor-suppressive pseudogene.…”

Section: Discussionmentioning

confidence: 99%

LncRNA weighted gene co-expression network analysis reveals novel biomarkers related to prostate cancer metastasis

et al. 2022

View full text Add to dashboard Cite

Background Most prostate cancer patients die from metastasis and lack accurate efficacious biomarkers to monitor the disease behavior, optimize treatment and assess prognosis. Herein, we aimed to identify meaningful lncRNA biomarkers associated with prostate cancer metastatic progression. Methods By repurposing microarray probes, 11,624 lncRNAs in prostate cancer were obtained from Gene Expression Omnibus database (GSE46691, N = 545; GSE29079, N = 235; GSE94767, N = 130). Weighted gene co-expression network analysis was applied to determine the co-expression lncRNA network pertinent to metastasis. Hub lncRNAs were screened. RNA-seq and clinical data from the Cancer Genome Atlas prostate cancer (TCGA-PRAD) cohort (N = 531) were analyzed. Transwell assay and bioinformatic analysis were performed for mechanism research. Results The high expression levels of nine hub lncRNAs (FTX, AC005261.1, NORAD, LINC01578, AC004542.2, ZFAS1, EBLN3P, THUMPD3-AS1, GAS5) were significantly associated with Gleason score and increased probability of metastatic progression. Among these lncRNAs, ZFAS1 had the consistent trends of expression in all of the analysis from different cohorts, and the Kaplan-Meier survival analyses showed higher expression of ZFAS1 was associated with shorter relapse free survival. In-vitro studies confirmed that downregulation of ZFAS1 decreased prostate cancer cell migration. Conclusion We offered some new insights into discovering lncRNA markers correlated with metastatic progression of prostate cancer using the WGCNA. Some may serve as potential prognostic biomarkers and therapeutic targets for advanced metastatic prostate cancer.

show abstract

Long non-coding RNA ZFAS1 regulates cell proliferation and invasion in cervical cancer via the miR-190a-3p/KLF6 axis

Cited by 15 publications

References 52 publications

lncRNA ZFAS1 Promotes HMGCR mRNA Stabilization via Binding U2AF2 to Modulate Pancreatic Carcinoma Lipometabolism

lncRNA ZFAS1 Promotes HMGCR mRNA Stabilization via Binding U2AF2 to Modulate Pancreatic Carcinoma Lipometabolism

The targeting of DAPK1 with miR‐190a‐3p promotes autophagy in trophoblast cells

LncRNA weighted gene co-expression network analysis reveals novel biomarkers related to prostate cancer metastasis

Contact Info

Product

Resources

About