Long non-coding RNAs in retinoblastoma

Yang, Ming; Wei, Wenbin

doi:10.1016/j.prp.2019.152435

Cited by 35 publications

(22 citation statements)

References 70 publications

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“…Recent studies indicate that numerous lncRNAs are dysregulated in RB and affect the aggressive characteristics of RB cells by serving as oncogenes or tumor suppressors [22][23][24]. Underexpressed miRNAs typically exert tumor-suppressive effects on the progression of RB, whereas overexpressed miRNAs play oncogenic roles [25]. Accordingly, some lncRNAs may serve as attractive biomarkers of (and/or therapeutic targets in) RB.…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA LINC00324 promotes retinoblastoma progression by acting as a competing endogenous RNA for microRNA-769-5p, thereby increasing STAT3 expression

Dong

Wan

Yan

et al. 2020

Aging

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Long intergenic non-protein-coding RNA 324 (LINC00324) is abnormally expressed in multiple human cancer types and plays an important role in cancer initiation and progression. This study showed that LINC00324 was expressed at higher levels in retinoblastoma (RB) tumors and cell lines than in control samples. Increased LINC00324 expression closely correlated with the TNM stage, optic nerve invasion, and shorter overall survival among patients with RB. The knockdown of LINC00324 decreased RB cell proliferation, colony formation, migration, and invasion, and promoted apoptosis and cell cycle arrest in vitro as well as hindered tumor growth in vivo. With respect to the mechanism, LINC00324 acted as a competing endogenous RNA for microRNA-769-5p (miR-769-5p) in RB cells. The mRNA of signal transducer and activator of transcription 3 (STAT3) was identified as a direct target of miR-769-5p in RB cells. Rescue experiments indicated that restoration of STAT3 expression attenuated the tumor-suppressive actions of miR-769-5p in RB cells. Downregulation of miR-769-5p or restoration of STAT3 almost completely reversed the effects of LINC00324 knockdown on RB cells. Our findings describe a novel RB-related LINC00324-miR-769-5p-STAT3 axis that is implicated in the malignancy of RB in vitro and in vivo. This study may point to innovative therapeutic targets in RB.

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Section: Introductionmentioning

confidence: 99%

Long noncoding RNA LINC00324 promotes retinoblastoma progression by acting as a competing endogenous RNA for microRNA-769-5p, thereby increasing STAT3 expression

Dong

Wan

Yan

et al. 2020

Aging

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“…Long non‐coding RNAs (lncRNAs) are defined as non‐protein coding transcripts longer than 200 nucleotides . Evidence suggests that lncRNAs play important roles in retinoblastoma diagnosis and prognosis .…”

Section: Introductionmentioning

confidence: 99%

“…Long non-coding RNAs (lncRNAs) are defined as non-protein coding transcripts longer than 200 nucleotides. 8 Evidence suggests that lncRNAs play important roles in retinoblastoma diagnosis and prognosis. [9][10][11][12] The lymphoid enhancer binding factor 1 antisense RNA 1 (LEF1-AS1) is a highly conserved transcript located in the human chromosome 4q25.…”

Section: Introductionmentioning

confidence: 99%

Long non‐coding RNA LEF1‐AS1 is involved in the progression of retinoblastoma through regulating the Wnt/β‐catenin pathway

Qin

2020

Clin Exp Pharma Physio

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The lymphoid enhancer binding factor 1 antisense RNA 1 (LEF1‐AS1) has been suggested to function as a tumour‐associated lncRNA in several types of human cancers, but there is no study to date about the role of LEF1‐AS1 in retinoblastoma. In our study, LEF1‐AS1 expression was increased in retinoblastoma tissues and cell lines compared with paired adjacent normal tissues and the retinal pigment epithelial cell line, respectively. Meanwhile, we found that patients with retinoblastoma with IIRC D‐E or undifferentiated type had notably higher levels of LEF1‐AS1 expression than those with IIRC A‐C or differentiated type. High LEF1‐AS1 expression predicted poor disease‐free survival in patients with retinoblastoma. The in vitro assays suggested that silencing of LEF1‐AS1 suppressed retinoblastoma cell proliferation, migration, and invasion through regulating the Wnt/β‐catenin pathway. In conclusion, LEF1‐AS1 functions as an oncogenic lncRNA in retinoblastoma.

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“…RB is the most frequent intraocular cancer of childhood with highly aggressive and metastatic but a rare disease (about 4% of all pediatric malignancies) [22]. Interestingly, increasing studies demonstrated that lncRNAs play important roles in promoting or suppressing RB progression, metastasis, diagnosis, and prognosis, such as HOTAIR, NEAT1, XIST, MEG3, and H19 [23]. XIST, a widely studied lncRNA, is dysregulated and serves as a potential biomarker in multiple cancers [24], but research on the mechanism of XIST is not well studied in RB.…”

Section: Discussionmentioning

confidence: 99%

XIST promotes cell proliferation and invasion by regulating miR-140-5p and SOX4 in retinoblastoma

et al. 2020

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Background: Retinoblastoma (RB) is the most common intraocular malignancy in children. Long non-coding RNA X-inactive specific transcript (lncRNA XIST) has been reported to be associated with RB, but research on the mechanism of XIST is not well studied. Methods: Expressions of XIST, microRNA-140-5p (miR-140-5p), and sex-determining region Y-related high-mobility group box 4 (SOX4) were analyzed by qRT-PCR or Western blot. Relationships of XIST, SOX4, and miR-140-5p were evaluated by dual-luciferase reporter assay and Spearman's analysis. Cell Counting Kit-8 (CCK-8) and Transwell assay were performed to assess the function of XIST on RB cell proliferation and invasion. Results: In RB tissues, XIST and SOX4 expressions were obviously increased, but the miR-140-5p expression was markedly reduced. XIST expression was positively related to SOX4 expression while negatively correlated with miR-140-5p expression, and negative correlation was exhibited between miR-140-5p and SOX4 expression in RB tissues. XIST was confirmed to directly bind to miR-140-5p, and SOX4 was one target of miR-140-5p. XIST knockdown could impede RB cell proliferation and invasion, while miR-140-5p inhibition reversed the effects. In addition, XIST overexpression or miR-140-5p inhibition could abrogate the inhibition of SOX4 silencing on cell proliferation and invasion of RB cells. Conclusions: XIST was obviously increased in RB tissues and cells, and XIST inhibition repressed the proliferation and invasion of RB cells by miR-140-5p/SOX4 axis, which may provide new understandings of the XIST molecular mechanism in RB.

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Long non-coding RNAs in retinoblastoma

Cited by 35 publications

References 70 publications

Long noncoding RNA LINC00324 promotes retinoblastoma progression by acting as a competing endogenous RNA for microRNA-769-5p, thereby increasing STAT3 expression

Long noncoding RNA LINC00324 promotes retinoblastoma progression by acting as a competing endogenous RNA for microRNA-769-5p, thereby increasing STAT3 expression

Long non‐coding RNA LEF1‐AS1 is involved in the progression of retinoblastoma through regulating the Wnt/β‐catenin pathway

XIST promotes cell proliferation and invasion by regulating miR-140-5p and SOX4 in retinoblastoma

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