Long non‐coding <scp>RNA HOTTIP</scp> is frequently up‐regulated in hepatocellular carcinoma and is targeted by tumour suppressive miR‐125b

Tsang, Felice Ho‐Ching; Au, Shannon Wing Ngor; Wei, Lai; Fan, Dorothy Ngo-Yin; Lee, Joyce M.F.; Wong, Carmen Chak‐Lui; Ng, Irene Oi–Lin; Wong, Chun–Ming

doi:10.1111/liv.12746

Cited by 138 publications

(96 citation statements)

References 26 publications

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“…In ovarian cancer, miR-125a and miR-125b could inhibit ovarian cancer cell migration, invasion and proliferation by regulating EIF4E-binding protein 1 (24). A study on HCC revealed that the tumor suppressive miR-125b could negatively regulate the expression of long non-coding RNA HOTTIP (25), and low expression of miR-125b was a prognostic marker for poor disease status and outcome (13). It was also revealed that miR-125b could suppress gastric cancer proliferation and invasion by targeting MCL1, and the low expression of miR-125b was associated with advanced clinical stage, lymph node metastases and poor clinical outcomes.…”

Section: Discussionmentioning

confidence: 99%

“…A number of studies about miR-125b indicated that miR-125b acted as a tumor suppressor in osteosarcoma, ovarian cancer, hepatocellular carcinoma (HCC) and gastric cancer (15,(24)(25)(26). In ovarian cancer, miR-125a and miR-125b could inhibit ovarian cancer cell migration, invasion and proliferation by regulating EIF4E-binding protein 1 (24).…”

Section: Discussionmentioning

confidence: 99%

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miR-125b is associated with renal cell carcinoma cell migration, invasion and apoptosis

Jin

Zeng

et al. 2017

Oncology Letters

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Abstract. MicroRNA (miR)-125b has been identified as deregulated in a number of types of cancer. Previous studies have detected the expression of miR-125b in clear cell renal cell carcinoma (ccRCC) tissues by in situ hybridization and revealed that miR-125b was upregulated in ccRCC tissues, and was associated with recurrence and survival of patients with ccRCC. However, the function of miR-125b in RCC remains unclear. Thus, the expression of miR-125b was detected with quantitative polymerase chain reaction (qPCR) in 24 paired RCC and adjacent normal tissues. The result of qPCR showed that miR-125b was upregulated in RCC tissues. Furthermore, the function of miR-125b in RCC (786-O and ACHN) cells was detected by transfecting miR-125 mimic or inhibitor to upregulate or downregulate miR-125b expression. Cell proliferation assays (MTT and Cell Counting Kit-8), cell mobility assays (cell scratch and Transwell assay) and a cell apoptotic assay (flow cytometry assay) were performed to assess the function of miR-125b on RCC cells. Results from the assays demonstrated that overexpression of miR-125b could promote cell migration and invasion, and reduce the cell apoptotic rate. It was also revealed that downregulation of miR-125b could reduce cell migration and invasion, and induce cell apoptosis. However, the results of the cell proliferation assay revealed that miR-125b had no significant effect on cell proliferation. Not only could miR-125b predict recurrence and survival of ccRCC; the present study revealed that miR-125b could regulate RCC cell migration, invasion and apoptosis. Additional studies are required to determine the mechanism of miR-125b in RCC cells and define the target genes of miR-125b in RCC.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

miR-125b is associated with renal cell carcinoma cell migration, invasion and apoptosis

Jin

Zeng

et al. 2017

Oncology Letters

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“…Knockdown of HOTTIP significantly suppressed the expression of a number of HOXA genes Moreover, in human HCCs, HOTTIP expression negatively correlated with that of miR-125b, which is a post-transcriptional regulator of HOTTIP [31] (Figure 1e). miR-141 and miR-148a show an inhibitory effect on the expression of DNA methyltransferase 1 (DNMT-1) and thus induces the overexpression of MEG3 [32] (Figure 2c).…”

Section: Interaction With Mirnasmentioning

confidence: 99%

Roles of long noncoding RNAs in Hepatocellular Carcinoma

2016

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Long noncoding RNAs (lncRNAs) are nonprotein coding transcripts longer than 200 nucleotides. Aberrant expression of lncRNAs has been found to be associated with hepatocellular carcinoma, one of the most malignant tumors. In this paper, we give a systematic and comprehensive review of existing literature about the involvement of lncRNAs in hepatocellular carcinoma. To date, evidence suggests that a number of lncRNAs, including HEIH, H19, HOTAIR, MALAT1, and PVT1, may regulate the transcription of target genes by recruiting histone-modifying complexes. Under certain circumstances, lncRNAs form RNA-dsDNA triplexes. Certain lncRNAs, such as HULC, HOTAIR, H19, HOTTIP and PTENP1, exhibit their biological roles by associating with microRNAs (miRNAs). In addition, by complementary base pairing with mRNAs or forming complexes with RNA binding proteins (RBPs), lncRNA-ATB, MALAT1 and PCNA-AS1 may mediate mRNA stability and splicing. In conclusion, interactions with DNA, RNA and proteins appears to be involved in lncRNAs' participation in tumorigenesis and developmental processes related to hepatocellular carcinoma.

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“…It has been reported to directly bind the adaptor protein WD repeat-containing protein 5 (WDR5) and to target WDR5/mixed lineage leukemia complexes, driving histone H3 lysine 4 trimethylation and gene transcription of distal HOXA genes (13). Several previous studies have reported that, compared with normal adjacent tissues (NATs), HOTTIP expression was significantly increased in skin, hepatocellular, pancreatic, lung and tongue squamous cell cancer tissues (14)(15)(16)(17)(18)(19)(20). HOTTIP may be involved in the progression of these cancers.…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA HOXA transcript at the distal tip as a biomarker for gastric cancer

Yang

Yan

et al. 2017

Oncology Letters

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Abstract.A long non-coding RNA named HOXA transcript at the distal tip (HOTTIP) has been reported to be significantly increased in several cancers, including hepatocellular cancer, pancreatic cancer and lung cancer. However, the clinical value of HOTTIP expression in gastric cancer remains unknown. The present study aimed to investigate HOTTIP expression levels in gastric cancer and to elucidate its clinical significance. Reverse transcription polymerase chain reaction was used to assess the expression level of HOTTIP in gastric cancer cell lines and tissues. In a cohort of 94 patients with gastric cancer, HOTTIP expression was significantly lower in cancer tissues compared with the normal adjacent tissues. In addition, receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic value of HOTTIP in gastric cancer, and the area under the ROC curve was 0.767. In conclusion, the results of the present study indicated that HOTTIP may be a predictive biomarker for gastric cancer.

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Long non‐coding RNA HOTTIP is frequently up‐regulated in hepatocellular carcinoma and is targeted by tumour suppressive miR‐125b

Abstract: HOTTIP is a novel oncogenic lncRNA, which negatively regulated by miR-125b. Overexpression of HOTTIP contributes to hepatocarcinogenesis by regulating the expression of its neighbouring protein-coding genes.

Cited by 138 publications

References 26 publications

miR-125b is associated with renal cell carcinoma cell migration, invasion and apoptosis

miR-125b is associated with renal cell carcinoma cell migration, invasion and apoptosis

Roles of long noncoding RNAs in Hepatocellular Carcinoma

Long non-coding RNA HOXA transcript at the distal tip as a biomarker for gastric cancer

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