Long Noncoding RNA-1604 Orchestrates Neural Differentiation through the miR-200c/ZEB Axis

Weng, Rong; Lu, Chenqi; Liu, Xiaoqin; Li, Guoping; Lan, Yuanyuan; Qiao, Jing; Bai, Mingliang; Wang, Zhaojie; Guo, Xudong; Ye, Dan; Jiapaer, Zeyidan; Yang, Yiwei; Xia, Chenliang; Wang, Guiying

doi:10.1002/stem.2749

Cited by 35 publications

(29 citation statements)

References 53 publications

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“…For lncRNAs and mRNA, the RNA‐sequencing library generation, workflow, and data analysis were performed as previously described [18]. The small RNA sequencing including miRNAs was also performed.…”

Section: Methodsmentioning

confidence: 99%

Whole‐transcriptome sequencing uncovers core regulatory modules and gene signatures of human fetal growth restriction

Wang

Yang

et al. 2020

Clinical & Translational Med

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BackgroundFetal growth restriction (FGR) contributes the primary cause of perinatal mortality and morbidity with impacts on the long‐term health. To determine the core gene expression network and gene signatures, which in combination with ultrasound confirmation will more effectively differentiate constitutionally normal small for gestational age and pathological FGR groups, we performed RNA sequencing for protein‐coding genes, lncRNAs, and small RNAs in a case–control study of umbilical cord blood. ResultsFive pairs of FGR case and control umbilical cord blood samples were used for RNA sequencing and weighted gene co‐expression network analysis (WGCNA). Results showed that 339 mRNAs, 295 lncRNAs, and 13 miRNAs were significantly differentially expressed between FGR cases and controls. Bioinformatics analysis indicated that these differentially expressed molecules were mainly involved in metabolism, neural, cardiac, and immune systems, and identified 18 WGCNA modules for FGR. Further quantitative verification was performed using umbilical cord blood and maternal peripheral blood from 12 pairs of FGR cases and controls. The logistic regression and receiver operating characteristic curve indicated that RP11_552M6.1, LINC01291, and Asgr1 in umbilical cord blood, while Sfrp2, miR‐432‐5p, and miR‐1306‐3p in maternal peripheral blood had potential significance for FGR. ConclusionsWe comprehensively profiled the whole‐transcriptome landscape of human umbilical cord blood with FGR, constructed the core WGCNA modules, and delineated the critical gene signatures of FGR. These findings provide key insight into intrauterine perturbations and candidate signatures for FGR.

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Section: Methodsmentioning

confidence: 99%

Whole‐transcriptome sequencing uncovers core regulatory modules and gene signatures of human fetal growth restriction

Wang

Yang

et al. 2020

Clinical & Translational Med

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“…Accumulation research have indicated that lncRNAs serve as miRNAs "sponges" to influence gene expression base on the ceRNA regulation theory. [18][19][20] lncRNA SNHG1 sponges miR-15b-5p triggers SIAH1 activation and further leads to α-synuclein aggregation and neurotoxicity. 21 Likewise, RAB3IP induces HOTAIR acts as a sponge of miR-126-5p aggravates the progression of PD.…”

mentioning

confidence: 99%

LncRNA MALAT1 targeting miR‐124‐3p regulates DAPK1 expression contributes to cell apoptosis in Parkinson's Disease

Gong

Jin

et al. 2020

J of Cellular Biochemistry

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Death associated protein kinase 1 (DAPK1) was initially discovered in the progress of gamma-interferon induced programmed cell death, it is a key factor in the central nervous system, including Parkinson's disease (PD). However, the underlying mechanisms of DAPK1 in PD remain unclear and this research work aims to explore the potential mechanisms of DAPK1 in PD. In the study, we exposed SH-SY5Y cells to MPP + and treated mice with MPTP to investigate the roles of DAPK1 in PD and the underlying mechanisms. The results indicated that the expression of DAPK1 is significantly upregulated and negatively correlated with miR-124-3p levels in SH-SY5Y cells treated by MPP + , and miR-124-3p mimics could effectively inhibit DAPK1 expressions and alleviate MPP +-induced cell apoptosis. In addition, knockdown MALAT1 reduces the levels of DAPK1 and the ratio of SH-SY5Y cell apoptosis, which is reversed via miR-124-3p inhibitor in vitro. Similarly, knockdown MALAT1 could improve behavioral changes and reduce apoptosis by miR-124-3p upregulation and DAPK1 downregulation in MPTP induced PD mice. Taken together, our data showed that lncRNA MALAT1 positively regulates DAPK1 expression by targeting miR-124-3p, and mediates cell apoptosis and motor disorders in PD. In summary, these results suggest that MALAT1/miR-124-3p /DAPK1 signaling cascade mediates cell apoptosis in vitro and in vivo, which may provide experimental evidence of developing potential therapeutic strategies for PD.

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“…LncRNA-1604 deficiency inhibits ectoderm differentiation and decreases the expression of neural progenitor cell markers (Sox1,Nestin,Zfp521,Pax6,. Further luciferase and RIP assays demonstrate that lncRNA-1604 can interact and sponge miR-200c to suppress the expression of the core transcription factors ZEB1/2 and the subsequently neural differentiation (Weng et al, 2018).…”

Section: Lncrna In Neurogenesismentioning

confidence: 98%

LncRNA Functions as a New Emerging Epigenetic Factor in Determining the Fate of Stem Cells

Chen

Wang

et al. 2020

Front. Genet.

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Pluripotent stem cells have broad applications in regenerative medicine and offer ideal models for understanding the biological process of embryonic development and specific diseases. Studies suggest that the self-renewal and multi-lineage differentiation of stem cells are regulated by a complex network consisting of transcription factors, chromatin regulators, signaling factors, and non-coding RNAs. It is of great interest to identify RNA regulatory factors that determine the fate of stem cells. Long non-coding RNA (lncRNA), a class of non-coding RNAs with more than 200 bp in length, has been shown to act as essential epigenetic regulators of stem cell pluripotency and specific lineage commitment. In this review, we focus on recent research progress related to the function and epigenetic mechanisms of lncRNA in determining the fate of stem cells, particularly pluripotency maintenance and lineage-specific differentiation. We discuss the role of the Oct4 and Sox2 promoter-interacting lncRNA as identified by Chromatin RNA In Situ reverse Transcription sequencing (CRIST-seq). Further understanding of their potential actions will provide a basis for the development of regenerative medicine for clinical application. This work offers comprehensive details and better understanding of the role of lncRNA in determining the fate of stem cells and paves the way for clinical stem cell applications.

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Long Noncoding RNA-1604 Orchestrates Neural Differentiation through the miR-200c/ZEB Axis

Cited by 35 publications

References 53 publications

Whole‐transcriptome sequencing uncovers core regulatory modules and gene signatures of human fetal growth restriction

Whole‐transcriptome sequencing uncovers core regulatory modules and gene signatures of human fetal growth restriction

LncRNA MALAT1 targeting miR‐124‐3p regulates DAPK1 expression contributes to cell apoptosis in Parkinson's Disease

LncRNA Functions as a New Emerging Epigenetic Factor in Determining the Fate of Stem Cells

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