Long Noncoding RNA 3632454L22Rik Contributes to Corneal Epithelial Wound Healing by Sponging miR-181a-5p in Diabetic Mice

Chen, Xiaxue; Hu, Jianzhang

doi:10.1167/iovs.62.14.16

Cited by 10 publications

(6 citation statements)

References 51 publications

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“… 31 Additionally, lncRNA XIST was found to promote extracellular matrix synthesis, proliferation, and migration in human skin fibroblasts following thermal injury by acting as a miR-29b-3p sponge and targeting COL1A1. 32 Moreover, a study by Chen and Hu 33 investigated the expression profile of differentially expressed lncRNAs and revealed that lncRNA 3632454L22RiK may positively impact diabetic corneal epithelial wound healing by sponging miR-181a-5p. Collectively, these findings underscore the intriguing and significant potential of lncRNAs to serve as a promising avenue for future investigations into wound healing processes.…”

Section: Discussionmentioning

confidence: 99%

Unraveling the Intricate Network of lncRNAs in Corneal Epithelial Wound Healing: Insights Into the Regulatory Role of linc17500

Cao,

Peng,

Wang

et al. 2024

Trans. Vis. Sci. Tech.

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Purpose Epigenetic mechanisms orchestrate a harmonious process of corneal epithelial wound healing (CEWH). However, the precise role of long non-coding RNAs (lncRNAs) as key epigenetic regulators in mediating CEWH remains elusive. Here, we aimed to elucidate the functional contribution of lncRNAs in regulating CEWH. Methods We used a microarray to characterize lncRNA expression profiling during mouse CEWH. Subsequently, the aberrant lncRNAs and their cis -associated genes were subjected to comprehensive Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Quantitative reverse transcription–polymerase chain reaction (RT-qPCR) and western blot analyses were performed to determine the expression profiles of key markers during CEWH. The in vivo effects of linc17500 on this process were investigated through targeted small interfering RNA (siRNA) injection. Post-siRNA treatment, corneal re-epithelialization was assessed, alongside the expression of cytokeratins 12 and 14 (Krt12 and Krt14) and Ki67. Effects of linc17500 on mouse corneal epithelial cell (TKE2) proliferation, cell cycle, and migration were assessed by multicellular tumor spheroids (MTS), 5-ethynyl-2′-deoxyuridine (EdU), flow cytometry, and scratch-wound assay, respectively. Results Microarray analysis revealed dysregulation of numerous lncRNA candidates during CEWH. Bioinformatic analysis provided valuable annotations regarding the cis -associated genes of these lncRNAs. In vivo experiments demonstrated that knockdown of linc17500 resulted in delayed CEWH. Furthermore, the knockdown of linc17500 and its cis -associated gene, CDC28 protein kinase regulatory subunit 2 ( Cks2 ), was found to impede TKE2 cell proliferation and migration. Notably, downregulation of linc17500 in TKE2 cells led to suppression of the activation status of Akt and Rb. Conclusions This study sheds light on the significant involvement of lncRNAs in mediating CEWH and highlights the regulatory role of linc17500 on TKE2 cell behavior. Translational Relevance These findings provide valuable insights for future therapeutic research aimed at addressing corneal wound complications.

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Section: Discussionmentioning

confidence: 99%

Unraveling the Intricate Network of lncRNAs in Corneal Epithelial Wound Healing: Insights Into the Regulatory Role of linc17500

Cao,

Peng,

Wang

et al. 2024

Trans. Vis. Sci. Tech.

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“…Chaaban et al detected a total of 2643 identifiable circRNAs expressed in both ischemic and normal conditions, and only 40 differentially expressed circRNAs between these two conditions by using rat retina RNA‐seq data 52 . The RNA‐seq technique may detect abundant RNA sequences in tissues, but it is not effective for the quantification and assessment of differential expression profiles between 2 conditions 53 . Characterised by circular junction reads and low tissue abundance, circRNAs are better detected and analysed using microarrays, which are more comprehensive and precise than RNA‐seq.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA expression profile and functional analysis of circUvrag in light‐induced photoreceptor degeneration

Kong,

Ding,

Wang

et al. 2024

Clinical Exper Ophthalmology

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BackgroundCircular RNAs (circRNAs) are implicated in retinal pathophysiology; however, their expression profiles and functions in photoreceptor apoptosis are largely unknown. We explored circRNA‐expression profiles and circUvrag (host gene: Uvrag, ultraviolet radiation resistance associated gene) function in light‐induced photoreceptor apoptosis.MethodsSprague–Dawley rats and 661 W photoreceptor cells were exposed to blue light to establish light‐induced photoreceptor degeneration. Differentially expressed circRNAs were identified using microarrays. Potential functions of dysregulated circRNAs were analysed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. CircUvrag expression and localization were evaluated using quantitative RT‐PCR and fluorescence in situ hybridization, respectively. CircUvrag overexpression and knockdown were induced using a plasmid and a small interfering RNA, respectively, and retinal function and structure were assessed using scotopic electroretinography, haematoxylin–eosin staining, and TUNEL staining. Microglial migration was assessed using IBA1 immunostaining. The apoptosis ratio of photoreceptor cells in vitro was detected using flow cytometry.ResultsWe identified 764 differentially expressed circRNAs, which were potentially related with the development of retinal structures, including neurons, dendrites, and synapses, and might participate in nervous‐system pathophysiology. Light exposure enriched circUvrag in the cytoplasm of photoreceptors in the outer nuclear layer (ONL). CircUvrag knockdown decreased photoreceptor apoptosis and microglial migration to the ONL after light exposure, preserving ONL thickness and a‐wave amplitude. In vitro, circUvrag knockdown inhibited photoreceptor apoptosis, although circUvrag overexpression slightly promoted photoreceptor apoptosis.ConclusionsCircUvrag knockdown attenuated light‐induced photoreceptor apoptosis, and might be a potential target in retinal degeneration.

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“…1) Symblepharon score -2) Corneal clarity 3) Vascularization DISCUSSION -Chen and Tseng conducted a study and concluded that a deficiency of limbal stem cells contributes to the triad of conjunctival epithelial ingrowth, corneal vascularization, and delayed healing with recurrent erosion. In partial limbal deficiency, corneal epithelium is still compromised, particularly when a large epithelial cell mass is removed [11]. Other study conducted by Anderson partial LSCD [12].However another study showed Amniotic membrane transplantation using fibrin glue to be a safe and effective method for cases with partial LSCD.…”

Section: Structural Outcomementioning

confidence: 99%

Outcome of LSCT With and Without Amt in Partial LSCD

Pachole

Yadav

2023

PIJR

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Purpose – To compare the outcomes of LSCT with and with out AMT in patients of partial LSCD following chemical burns. Method – 32 eyes with unilateral LSCD were randomized into two groups. Group A included 16 eyes who underwent LSCT with AMT while group B included 16 eyes who underwent LSCT alone at a tertiary hospital. Outcome were measured in terms of improvement of visual acuity , corneal clarity, vascularization and symblepharon reduction. Patients were followed up at end of 1st week,1 month and 3rd month,6th month and 1 year. Results – There was improvement of visual acuity in both groups. Also both group shows reduction in symblepharon score and corneal vascularization. Both group shows improvement in corneal clarity. Conclusions- Both surgical technique are useful treatment modalities in patients with partial LSCD following ocular chemical injury.

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Long Noncoding RNA 3632454L22Rik Contributes to Corneal Epithelial Wound Healing by Sponging miR-181a-5p in Diabetic Mice

Cited by 10 publications

References 51 publications

Unraveling the Intricate Network of lncRNAs in Corneal Epithelial Wound Healing: Insights Into the Regulatory Role of linc17500

Unraveling the Intricate Network of lncRNAs in Corneal Epithelial Wound Healing: Insights Into the Regulatory Role of linc17500

Circular RNA expression profile and functional analysis of circUvrag in light‐induced photoreceptor degeneration

Outcome of LSCT With and Without Amt in Partial LSCD

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