Long noncoding RNA AC073284.4 suppresses epithelial–mesenchymal transition by sponging miR‐18b‐5p in paclitaxel‐resistant breast cancer cells

Wang, Yue‐Yue; Liu, Yan; Yang, Shuo; Xu, Huijing; Chen, Tian‐Tian; Dong, Zheng‐Yuan; Chen, Sulian; Wang, Wen‐Rui; Yang, Qingling; Chen, Chang‐Jie

doi:10.1002/jcp.28887

Cited by 46 publications

(25 citation statements)

References 42 publications

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“…We used the GEO database to select mRNAs that were downregulated in SOC, identified potential lncRNA-miRNA-mRNA interaction networks based on the presence of specific binding sites, and reconstructed a comprehensive ceRNA network. Several recent studies demonstrated that ceRNA-based mechanisms may operate in all types of carcinoma [5,6,[17][18][19][20][21][22][23]. In the present study, among the miRNAs found to be downregulated in SOC, hsa-miR-195-5p and hsa-miR-497-5p were predicted to bind to LINC00284.…”

Section: Discussionsupporting

confidence: 54%

Construction and Investigation of an LINC00284-Associated Regulatory Network in Serous Ovarian Carcinoma

et al. 2020

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The low survival rate associated with serous ovarian carcinoma (SOC) is largely due to the lack of relevant molecular markers for early detection and therapy. Increasing experimental evidence has demonstrated that long noncoding RNAs (lncRNAs) are involved in cancer initiation and development, and a competitive endogenous RNA (ceRNA) hypothesis has been formulated. Therefore, the characterization of new lncRNA and lncRNA-related networks is crucial for early diagnosis and targeted therapy of SOC. Data on lncRNAs, mRNAs, and miRNAs with differential expression in SOC, compared to normal ovarian tissue, were obtained from the Gene Expression Omnibus (GEO) database. Data on lncRNA expression and clinical data in SOC were obtained from The Cancer Genome Atlas (TCGA). lncRNA-miRNA interactions were predicted by the miRBase database. Different online tools, i.e., TargetScan, RNA22, miRmap, microT, miRanda, StarBase, and PicTar, were cooperatively utilized to predict the mRNAs targeted by miRNAs. The plugin of BiNGO in Cytoscape and KOBAS 3.0 were used to conduct the functional and pathway enrichment analyses. The lncRNA, miRNAs, and mRNAs identified to be expressed at statistically significant and different levels between SOC and healthy fallopian tube tissues were further validated using qRT-PCR. A total of 4 lncRNAs (LINC00284, HAGLR, HCAT158, and BLACAT1) and 111 mRNAs were found to be upregulated in SOC tissues compared to normal tissues, based on the GEO database. LINC00284 was found to be highly expressed in SOC, in association with the upregulation of the transcription factor SOX9. The high LINC00284 expression was associated with poor prognosis and proved to be an independent risk factor in patients with SOC, based on TCGA database. The qRT-PCR validation results closely recapitulated the expression profiles and prognostic scores of the aforementioned bioinformatic analyses. The LINC00284-related ceRNA network was found to be associated with SOC carcinogenesis by biofunctional analysis. In conclusion, the LINC00284-related ceRNA network may provide valuable information on the mechanisms of SOC initiation and progression. Importantly, LINC00284 proved to be a new potential prognostic biomarker for SOC.

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Section: Discussionsupporting

confidence: 54%

Construction and Investigation of an LINC00284-Associated Regulatory Network in Serous Ovarian Carcinoma

et al. 2020

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“…MiRNA microarray assay revealed that a total of 22 microRNAs were upregulated in GBC-SD cells when PVT1 was knockdown, and we selected miR-18b-5p which was related with the proliferation of GBC cells for further study. MiR-18b-5p has been reported to function as a tumor suppressor and be regulated by lncRNAs 19,20 . By analyzing the sequence of PVT1 and miR-18b-5p, no binding sites were found based on the principle of base pairing, so other mechanisms may exist by which PVT1 inhibited the expression of miR-18b-5p.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA PVT1 promoted gallbladder cancer proliferation by epigenetically suppressing miR-18b-5p via DNA methylation

et al. 2020

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Gallbladder cancer (GBC) accounts for 85–90% malignancies of the biliary tree worldwide. Considerable evidence has demonstrated that dysregulation of lncRNAs is involved in the progression of cancer. LncRNA PVT1 has been reported to play important roles in various cancers, but its role in gallbladder cancer remains unknown. In the present study, we found that PVT1 was upregulated in GBC tissues and cells, and its upregulation was related with poor prognosis in GBC patients. PVT1 promoted GBC cells proliferation in vitro and in vivo. Mechanistically, PVT1 recruited DNMT1 via EZH2 to the miR-18b-5p DNA promoter and suppressed the transcription of miR-18b-5p through DNA methylation. Moreover, HIF1A was proved to be the downstream target gene of miR-18b-5p and PVT1 regulated GBC cells proliferation via HIF1A. In conclusion, our studies clarified the PVT1/miR-18b-5p/HIF1A regulation axis and indicated that PVT1 could be a potential therapeutic target for GBC.

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“…Long non-coding RNAs (lncRNAs) are a group of highly conserved transcripts of >200 nucleotides, with no protein coding ability (6)(7)(8). An increasing number of studies have documented the involvement of lncRNAs in regulating the biological behaviors of cancer cells (9,10). lncRNAs can be categorized as oncogenes and/or tumor suppressors depending on the specific malignancy (11,12), and the aberrant expression of lncRNAs can be used to evaluate the tumorigenesis, progression and relapse of malignant tumors.…”

Section: Introductionmentioning

confidence: 99%

LINC00473 contributes to the radioresistance of esophageal squamous cell carcinoma by regulating microRNA‑497‑5p and cell division cycle 25A

Liu

Qiao

et al. 2020

Int J Mol Med

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Long non-coding RNA (lncRNA) LINc00473 plays a carcinogenic role in a variety of different tumor types. Nevertheless, the mechanisms through which LINc00473 regulates the radiosensitivity of esophageal squamous cell carcinoma (EScc) cells remains elusive. In the present study, reverse transcription-quantitative PcR was used to quantify the expression of LINc00473, microRNA (miRNA/miR)-497-5p and cell division cycle 25A (cdc25A) in EScc tissues. The association between LINc00473 expression and the clinicopathological characteristics of patients with EScc was also assessed. Furthermore, cell counting kit-8 and colony formation assays were carried out to monitor the proliferation of EScc cells exposed to X-ray radiation. A dual-luciferase reporter assay was also conducted to analyze the interaction between LINc00473 and miR-497-5p, as well as the interaction between CDC25A and miR-497-5p. The findings of the present study demonstrated that in EScc tissues and cells, the expression levels of LINC00473 and CDC25A were significantly upregulated, while the expression of miR-497-5p was downregulated. The high expression level of LINc00473 was associated with a higher T stage, lymph node metastasis stage and a lower tumor differentiation grade in patients with EScc. Following irradiation, transfection with miR-497-5p mimics reduced the promoting effect of LINc00473 overexpression on EScc cell proliferation, and partially impeded the resistance of EScc cells to X-ray radiation induced by LINc00473 overexpression. Moreover, transfection with miR-497-5p inhibitors partially alleviated the inhibitory effects of LINc00473 knockdown on cellular proliferation, and partly reversed the sensitivity of cells to X-ray irradiation induced by LINc00473 knockdown. Furthermore, it was confirmed that miR-497-5p was able to bind LINc00473 and the 3'-untranslated region of CDC25A. On the whole, the findings of the present study demonstrate that LINc00473 reduces the radiosensitivity of EScc cells by modulating the miR-497-5p/cdc25A axis.

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Long noncoding RNA AC073284.4 suppresses epithelial–mesenchymal transition by sponging miR‐18b‐5p in paclitaxel‐resistant breast cancer cells

Cited by 46 publications

References 42 publications

Construction and Investigation of an LINC00284-Associated Regulatory Network in Serous Ovarian Carcinoma

Construction and Investigation of an LINC00284-Associated Regulatory Network in Serous Ovarian Carcinoma

Long noncoding RNA PVT1 promoted gallbladder cancer proliferation by epigenetically suppressing miR-18b-5p via DNA methylation

LINC00473 contributes to the radioresistance of esophageal squamous cell carcinoma by regulating microRNA‑497‑5p and cell division cycle 25A

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