Long noncoding RNA ATB promotes ovarian cancer tumorigenesis by mediating histone H3 lysine 27 trimethylation through binding to EZH2

Chen, Xuejuan; An, Na

doi:10.1111/jcmm.15329

Cited by 8 publications

(7 citation statements)

References 36 publications

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“…Accumulating evidence has indicated that lncRNAs determine cancer cell fate through the regulation of target gene expression via interaction with specific RNAbinding proteins, such as histone modification enzymes, transcription factors, and alternative splicer [35][36][37][38]. The splicing factor SR-rich SRSF3 displays unique RNA binding properties and is upregulated in various cancer types, including breast cancer, ovarian cancer, and gastric cancer (for review, see [39]), functioning as an oncogenic molecule in these cancers.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: LNCAROD enhances hepatocellular carcinoma malignancy by activating glycolysis through induction of pyruvate kinase isoform PKM2

Jia

Wang

Lin

et al. 2021

J Exp Clin Cancer Res

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Background Mounting evidence has suggested the essential role of long non-coding RNAs (lncRNAs) in a plethora of malignant tumors, including hepatocellular carcinoma. However, the underlyling mechanisms of lncRNAs remain unidentified in HCC. The present work was aimed to explore the regulatory functions and mechanisms of LncRNA LNCAROD in HCC progression and chemotherapeutic response. Methods The expression of LNCAROD in HCC tissues and cell lines were detected by quantitative reverse transcription PCR (qPCR). Cancer cell proliferation, migration, invasion, and chemoresistance were evaluated by cell counting kit 8 (CCK8), colony formation, transwell, and chemosensitivity assays. Methylated RNA immunoprecipitation qRCR (MeRIP-qPCR) was used to determine N6-methyladenosine (m6A) modification level. RNA immunoprecipitation (RIP) and RNA pull down were applied to identify the molecular sponge role of LNCAROD for modulation of miR-145-5p via the competing endogenous RNA (ceRNA) mechanism, as well as the interaction between LNCAROD and serine-and arginine-rich splicing factor 3 (SRSF3). The interaction between insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) and LNCAROD was also identified by RIP assay. Gain- or-loss-of-function assays were used to identify the function and underlying mechanisms of LNCAROD in HCC. Results We found that LNCAROD was significantly upregulated and predicted a poorer prognosis in HCC patients. LNCAROD upregulation was maintained by increased m6A methylation-mediated RNA stability. LNCAROD significantly promoted HCC cell proliferation, migration, invasion, and chemoresistance both in vitro and in vivo. Furthermore, mechanistic studies revealed that pyruvate kinase isoform M2 (PKM2)-mediated glycolysis enhancement is critical for the role of LNACROD in HCC. According to bioinformatics prediction and our experimental data, LNCAROD directly binds to SRSF3 to induce PKM switching towards PKM2 and maintains PKM2 levels in HCC by acting as a ceRNA against miR-145-5p. The oncogenic effects of LNCAROD in HCC were more prominent under hypoxia than normoxia due to the upregulation of hypoxia-triggered hypoxia-inducible factor 1α. Conclusions In summary, our present study suggests that LNCAROD induces PKM2 upregulation via simultaneously enhancing SRSF3-mediated PKM switching to PKM2 and sponging miR-145-5p to increase PKM2 level, eventually increasing cancer cell aerobic glycolysis to participate in tumor malignancy and chemoresistance, especially under hypoxic microenvironment. This study provides a promising diagnostic marker and therapeutic target for HCC patients.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: LNCAROD enhances hepatocellular carcinoma malignancy by activating glycolysis through induction of pyruvate kinase isoform PKM2

Jia

Wang

Lin

et al. 2021

J Exp Clin Cancer Res

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“…A recent study identified the promotion of lnc_000231 in cervical cancer, which acts by interacting with miR ‐ 497 ‐ 5p 30 . The lncRNA ATB binds to EZH2 and downregulates the expression of DAB2IP, CDH1, LATS2, FOXC1 and CDX1, thus facilitating the progression of ovarian cancer 31 . Furthermore, our study showed that LOXL1 ‐ AS1 is highly expressed in glioma tissues and cells, and its downregulation repressed the proliferation, metastasis and VM ability of these cells.…”

Section: Discussionsupporting

confidence: 51%

“… 30 The lncRNA ATB binds to EZH2 and downregulates the expression of DAB2IP, CDH1, LATS2, FOXC1 and CDX1, thus facilitating the progression of ovarian cancer. 31 Furthermore, our study showed that LOXL1 ‐ AS1 is highly expressed in glioma tissues and cells, and its downregulation repressed the proliferation, metastasis and VM ability of these cells. These results confirmed that LOXL1‐AS1 has an aggressive role in glioma.…”

Section: Discussionmentioning

confidence: 66%

LOXL1‐AS1 communicating with TIAR modulates vasculogenic mimicry in glioma via regulation of the miR‐374b‐5p/MMP14 axis

Cai

et al. 2021

J Cellular Molecular Medi

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At present, growing evidence indicates that long non‐coding RNAs (lncRNAs) participate in the progression of glioma. The function of LOXL1‐AS1 in vasculogenic mimicry (VM) in glioma remains unclear. First, the expressions of TIAR, the lncRNA LOXL1‐AS1, miR‐374b‐5p and MMP14 were examined by qRT‐PCR and Western blot in both, glioma tissues and glioma cell lines. Proliferation, migration, invasion and tube formation assays were conducted to evaluate the roles of TIAR, LOXL1‐AS1, miR‐374b‐5p and MMP14 in malignant cellular behaviours in glioma cells. A nude mouse xenograft model and dual staining for CD34 and PAS were used to assess whether VM was affected by TIAR, LOXL1‐AS1 or miR‐374b‐5p in vivo. In this study, low levels of TIAR and high levels of LOXL1‐AS1 were found in glioma cells and tissues. TIAR downregulated the expression of LOXL1‐AS1 by destabilizing it. LOXL1‐AS1 acted like a miRNA sponge towards miR‐374b‐5p so that downregulation of the former greatly inhibited cell proliferation, migration, invasion and VM. Additionally, miR‐374b‐5p overexpression repressed malignant biological behaviours and VM in glioma by modifying MMP14. In summary, we demonstrated that TIAR combined with LOXL1‐AS1 modulates VM in glioma via the miR‐374b‐5p/MMP14 axis, revealing novel targets for glioma therapy.

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“…Increasing evidence has reported that the lncRNA ATB ( lnc-ATB ) promoted tumor progression in breast [ 99 ], prostate [ 100 ], and colon cancers [ 101 ], therefore suggesting lnc-ATB as a potential prognostic marker and therapeutic target in human cancers [ 102 ]. Chen et al have reported that levels of lnc-ATB were increased in ovarian cancer tissue and high levels of lnc-ATB were associated with poor outcomes of patients with ovarian cancer [ 103 ]. Further, the authors demonstrated that lnc-ATB directly interacts with EZH2 by RIP and RNA pull-down assays in human ovarian cancer cell lines SKOV3 and A2780.…”

Section: Non-coding Rnas Bound By Prc2 and Their Implications In Cancermentioning

confidence: 99%

Implications in Cancer of Nuclear Micro RNAs, Long Non-Coding RNAs, and Circular RNAs Bound by PRC2 and FUS

Swaminathan,

Rogel-Ayala,

Armich

et al. 2024

Cancers

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The eukaryotic genome is mainly transcribed into non-coding RNAs (ncRNAs), including different RNA biotypes, such as micro RNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs), among others. Although miRNAs are assumed to act primarily in the cytosol, mature miRNAs have been reported and functionally characterized in the nuclei of different cells. Further, lncRNAs are important regulators of different biological processes in the cell nucleus as part of different ribonucleoprotein complexes. CircRNAs constitute a relatively less-characterized RNA biotype that has a circular structure as result of a back-splicing process. However, circRNAs have recently attracted attention in different scientific fields due to their involvement in various biological processes and pathologies. In this review, we will summarize recent studies that link to cancer miRNAs that have been functionally characterized in the cell nucleus, as well as lncRNAs and circRNAs that are bound by core components of the polycomb repressive complex 2 (PRC2) or the protein fused in sarcoma (FUS), highlighting mechanistic aspects and their diagnostic and therapeutic potential.

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Long noncoding RNA ATB promotes ovarian cancer tumorigenesis by mediating histone H3 lysine 27 trimethylation through binding to EZH2

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 8 publications

References 36 publications

RETRACTED ARTICLE: LNCAROD enhances hepatocellular carcinoma malignancy by activating glycolysis through induction of pyruvate kinase isoform PKM2

RETRACTED ARTICLE: LNCAROD enhances hepatocellular carcinoma malignancy by activating glycolysis through induction of pyruvate kinase isoform PKM2

LOXL1‐AS1 communicating with TIAR modulates vasculogenic mimicry in glioma via regulation of the miR‐374b‐5p/MMP14 axis

Implications in Cancer of Nuclear Micro RNAs, Long Non-Coding RNAs, and Circular RNAs Bound by PRC2 and FUS

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