Long noncoding RNA BLACAT1 indicates a poor prognosis of colorectal cancer and affects cell proliferation by epigenetically silencing of p15

Abstract

Recently, a novel class of transcripts, long noncoding RNAs (lncRNAs), is being identified at a rapid pace. These RNAs have critical roles in diverse biological processes, including tumorigenesis. One of them, BLACAT1, a cancer-associated long noncoding RNA, exerts regulatory functions in various biological processes in cancer cells, however, the role of BLACAT1 in colon cancer remains unclear. Our experiments showed that increased BLACAT1 was an independent unfavorable prognostic indicator for colorectal cancer, and revealed that BLACAT1 knockdown significantly repressed proliferation, both in vitro and in vivo. Mechanistic investigations demonstrated that BLACAT1 had a key role in G1/G0 arrest, and showed that BLACAT1 can repress p15 expression by binding to EZH2, thus contributing to the regulation of CRC cell cycle and proliferation. Our results suggest that BLACAT1, as a cell cycle regulator, may serve as a potential target for colon cancer prevention and treatment in human CRC.

Long Non-Coding RNA SH3PXD2A-AS1 Promotes Cell Progression Partly Through Epigenetic Silencing P57 and KLF2 in Colorectal Cancer

“…P15 and P16 have also been identified as tumor-suppressive proteins in CRC [49, 51]. In this study, we reported that overexpression of P57 and KLF2 can inhibit proliferative ability of CRC cells, and cause G1/G0 phase arrest.…”

“…We also reported that lncRNA HOXA-AS2 mediated CRC proliferation partly through inhibiting P21 and KLF2 expression [30]. In addition, the effects of P15 and P16 on CRC progression have been studied previously [49-51]. …”

lncRNA FAM83H‑AS1 is associated with the prognosis of colorectal carcinoma and promotes cell proliferation by targeting the Notch signaling pathway

“…Pei et al (15) reported that downregulation of lncRNA CASC2 promoted cell proliferation and metastasis of bladder cancer by activating the Wnt/β-catenin signaling pathway. And also many lncRNAs, for instance, ROR, BLACAT1, ANCR, TUG1, are identified to be dysregulated and play critical role in the initial and progression of CRC (14,(16)(17)(18). FAM83H-AS1 has been identified to play critical roles in lung cancer and breast cancer (19,20).…”

“…For example, Xia et al (13) demonstrated that long noncoding RNA papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) inhibited proliferation and invasion of glioma cells by suppressing the Wnt/β-catenin signaling pathway. Su et al (14) illustrated that long noncoding RNA BLACAT1 indicated a poor prognosis of colorectal cancer and affected cell proliferation by epigenetically silencing p15. Pei et al (15) reported that downregulation of lncRNA CASC2 promoted cell proliferation and metastasis of bladder cancer by activating the Wnt/β-catenin signaling pathway.…”

lncRNA SNHG5 is associated with poor prognosis of bladder cancer and promotes bladder cancer cell proliferation through targeting p27

“…Currently, long non-coding RNAs (lncRNAs), newly identified members of the noncoding RNA family with length >200 nucleotides (nt), have been proposed (2)(3)(4). Accumulating documents have revealed that lncRNAs play a critical role in tumorigenesis and can be used as biomarkers for diagnosis or prediction of survival and recurrence in multiple cancers (5)(6)(7)(8)(9).…”