Long noncoding RNA DGCR5 represses hepatocellular carcinoma progression by inactivating Wnt signaling pathway

Abstract

Increasing studies have indicated that long noncoding RNAs (lncRNAs) exert important roles in hepatocellular carcinoma (HCC). Therefore, it is of great significance to identify the dysregulated lncRNAs in HCC. According to the previous reports, it has been suggested that DiGeorge syndrome critical region gene 5 (DGCR5) might participate in HCC and can serve as potential biomarker for HCC. In our current study, we concentrated on the biological function and roles of lncRNA‐DGCR5 in HCC. It was indicated that DGCR5 was decreased in HCC tissues and HCC cells including HepG2, Hep3B, MHCC‐97L, SNU‐449, and SNU‐182 cells compared with the normal human liver cell line LO2. Overexpression of DGCR5 was able to restrain HCC growth, migration, and invasion capacity in HepG2 and SNU‐449 cells. In addition, whether lncRNA‐DGCR5 can regulate Wnt/β‐catenin pathway during HCC progression is unclear. In our study, it was found that upregulation of DGCR5 inactivated Wnt signaling pathway through inhibiting β‐catenin, cyclin D1 and increasing GSK‐3β levels. Subsequently, in vivo tumor xenografts were established using HepG2 cells to investigate the function of DGCR5 in HCC development. Inconsistent with the in vitro findings, increase of DGCR5 dramatically suppressed HCC tumor progression in vivo. Taken these together, it was uncovered in our research that DGCR5 could play tumor suppressive role by targeting Wnt signaling in HCC progression.

LncRNA DGCR5 regulates the non‐small cell lung cancer cell growth, migration, and invasion through regulating miR‐211‐5p/EPHB6 axis

“…Moreover, a recent study revealed that the upregulation of DGCR5 was indicator for better outcome of bladder cancer and interacted with AT‐rich interaction domain 1A to regulate cancer cell progression . In addition, DGCR5 was demonstrated to inhibit the development of hepatocellular carcinoma through targeting the Wnt signaling pathway . Importantly, DGCR5 was also revealed to have tumor suppressive role in lung cancer…”

“…8 In addition, DGCR5 was demonstrated to inhibit the development of hepatocellular carcinoma through targeting the Wnt signaling pathway. 9 Importantly, DGCR5 was also revealed to have tumor suppressive role in lung cancer. 10 Previously, we showed increased miR-211 expression in NSCLC, however, we did not explore the upstream molecule that can target miR-211 at that time.…”

LINC00355:8 promotes cell proliferation and migration with invasion via the MiR-6777-3p/Wnt10b axis in Hepatocellular Carcinoma

“…An increasing number of studies have identified numerous lncRNAs associated with HCC, such as DGCR5 and lncRNA PCAT-1. These lncRNAs contribute to the regulation of the proliferation, migration and invasion of HCC cells [18][19]. Our previous study showed that LINC00355:8 expression is increased in HCC compared with normal liver tissue; however, the biological function of LINC00355:8 in HCC remains unknown [11].…”

<p>Large-Scale Analysis Reveals the Specific Clinical and Immune Features of DGCR5 in Glioma</p>

“…20 Increasing evidence has revealed that DGCR5 is a suppressor of lung cancer, cervical cancer, gastric cancer and hepatocellular carcinoma progression. [21][22][23][24] Moreover, down-regulation of DGCR5 correlates with poor prognosis in hepatocellular carcinoma and bladder cancer. 25,26 A recent study reported that DGCR5 inhibited the proliferation, aggressiveness phenotypes, and EMT of glioblastoma cell.…”