Long noncoding RNA DLEU1 aggravates pancreatic ductal adenocarcinoma carcinogenesis via the miR‐381/CXCR4 axis

Abstract

Recent evidence has highlighted that long noncoding RNAs (lncRNA) are associated with many diseases, particularly cancer. However, current understanding of the lncRNA deleted in lymphocytic leukemia 1 (DLEU1) in pancreatic ductal adenocarcinoma (PDAC) remains limited. Our studies indicated that the DLEU1 expression level was upregulated in PDAC tissue samples compared with adjacent normal tissue. Moreover, the aberrant overexpression of DLEU1 indicated poor prognosis of patients with PDAC. Loss-of-function experiments revealed that DLEU1 knockdown inhibited the proliferation, migration, and invasion of PDAC cells in vitro and decreased tumor growth in vivo. Bioinformatics analysis predicted that miR-381 potentially targeted the DLEU1 3′-untranslated region (UTR), suggesting an interaction between miR-381 and DLEU1. Furthermore, miR-381 also targeted the chemokine receptor-4 (CXCR4) messenger RNA 3′-UTR, which was validated by luciferase reporter assay. Taken together, our study demonstrated the oncogenic role of DLEU1 in clinical PDAC specimens and cellular experiments, showing the potential involvement of DLEU1/miR-381/CXCR4 pathway. These results provide novel insight into PDAC tumorigenesis. K E Y W O R D S CXCR4, long noncoding RNAs (lncRNA) DLEU1, miR-381, pancreatic ductal adenocarcinoma (PADC)

Long noncoding RNA DLEU1 aggravates osteosarcoma carcinogenesis via regulating the miR-671-5p/DDX5 axis

“…Loss-of-function experiments demonstrate that knockdown of DLEU1 inhibits the PDAC cells proliferation, migration, and invasion in vitro and suppresses the tumour growth in vivo. Further investigations indicate that DLEU1 aggravates PDAC carcinogenesis by sponging miR-381 [14]. Wang et al [15] demonstrated that DLEU1 contributes to the tumourigenesis and development of ovarian carcinoma by interacting with miR-490-3p.…”

“…Further mechanistic analysis and functional assays validate that DLEU1 induces cell proliferation, invasion, and cisplatin resistance of bladder cancer cells through sponging miR-99b. Gao et al [14] reported that DLEU1 expression level is upregulated in pancreatic ductal adenocarcinoma (PDAC) tissues compared with adjacent normal tissue. The aberrant expression of DLEU1 indicates poor prognosis of PDAC patients.…”

lncRNA deleted in lymphocytic leukaemia 1 (DLEU1) promotes the migration and invasion of human embryonic trophoblast cells

“…Moreover, we discovered that lncRNA DLEU1 silencing did not influence cell proliferation but dramatically suppressed the migratory and invasive ability of HTR8/SVneo cells, suggesting that lncRNA DLEU1 may play critical roles in placentation. Similarly, Gao and colleagues reported that underexpression of lncRNA DLEU1 could inhibit migration and invasion of pancreatic ductal adenocarcinoma cells (Gao et al, 2019). Accumulating evidence has indicated that cell migratory and invasive processes are strongly correlated with significant cytoskeleton remodelling (Fife et al, 2014).…”

SP1–DLEU1–miR-4429 feedback loop promotes cell proliferative and anti-apoptotic abilities in human glioblastoma

“…Based on existing studies, lncRNA is widely identified to modulate diverse biological behaviors and cellular processes in GBM, thereby functioning as a crucial regulator in the GBM development [10][11][12]. LncRNA deleted in lymphocytic leukemia 1 (DLEU1) was confirmed to be up-regulated in colorectal cancer [13], oral squamous cell carcinoma [14], ovarian cancer [15], pancreatic ductal adenocarcinoma [16] and gastric cancer [17], consistently exerting oncogenic role. Recently, transcription factor is reported to activate lncRNA transcription, thereby promoting lncRNA expression in tumors [18][19][20].…”