Long noncoding RNA HAS2‐AS1 promotes tumor progression in glioblastoma via functioning as a competing endogenous RNA

Zhang, Liqun; Wang, Hong; Xu, Meijie; Chen, Fangyu; Li, Wenkui; Hu, H.; Yuan, Quan; Su, Yue; Liu, Xiaoxuan; Wuri, Jimusi; Yan, Tao

doi:10.1002/jcb.29313

Cited by 28 publications

(23 citation statements)

References 40 publications

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“…HAS2-AS1 mediates hypoxia-induced invasiveness of oral squamous cell carcinoma [12]. In GBM, the reduction in HAS2-AS1 inhibits GBM cells migration and invasion in vitro and in vivo [24]. Therefore, we speculated that HAS2-AS1 might play a role as an oncogene in glioma.…”

Section: Discussionmentioning

confidence: 97%

The transcription factor USF1 promotes glioma cell invasion and migration by activating lncRNA HAS2-AS1

Wang

You

et al. 2020

Bioscience Reports

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Objective: The role of lncRNAs in tumor has been widely concerned. This study took HAS2-AS1 as a starting point to explore its expression in glioma and its role in the process of migration and invasion, providing a strong theoretical basis for mining potential therapeutic targets of glioma. Methods: Clinical data of glioma were obtained from TCGA database and differentially expressed lncRNAs were analyzed by edgeR. The hTFtarget database was used to predict the upstream transcription factors of HAS2-AS1 and the JASPAR website was used to predict the binding sites of human upstream transcription factor 1 (USF1) and HAS2-AS1. qRT-PCR was used to detect the expressions of HAS2-AS1 and USF1 in glioma tissues and cell lines. The effects of silencing HAS2-AS1 on the migration and invasion of cancer cells were verified by wound healing and Transwell invasion assays. The ChIP and dual luciferase reporter assays were applied to demonstrate the binding of USF1 and HAS2-AS1 promoter region. Western blot was used to detect the expressions of EMT-related proteins. Results: HAS2-AS1 was highly expressed in glioma tissues and cells, and was significantly associated with poor prognosis. Silencing HAS2-AS1 expression inhibited glioma cell migration, invasion and EMT. USF1 was highly expressed in glioma and positively correlated with HAS2-AS1. The transcription of HAS2-AS1 was activated by USF1 via binding to HAS2-AS1 promoter region, consequently potentiating the invasion and migration abilities of glioma cells. Conclusion: These results suggested that the transcription factor USF1 induced upregulation of lncRNA HAS2-AS1 and promoted glioma cell invasion and migration.

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Section: Discussionmentioning

confidence: 97%

The transcription factor USF1 promotes glioma cell invasion and migration by activating lncRNA HAS2-AS1

Wang

You

et al. 2020

Bioscience Reports

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“…lncRNAs are highly conserved non-coding RNA transcripts of >200 nucleotides in length, which have attracted attention because of the discovery in recent years of their association with the process of tumor formation, invasion and metastasis ( 10 , 11 ). lncRNAs can fold into complex three-dimensional structures and interact specifically with transcription factors, histones and chromatin-modifying proteins, thereby affecting the expression of other genes ( 12 , 13 ). Several lncRNAs have been demonstrated to participate in the occurrence and progression of OSCC, including H1 and HAS2-AS1.…”

Section: Discussionmentioning

confidence: 99%

lncRNA MCM3AP‑AS1 promotes the development of oral squamous cell carcinoma by inhibiting miR‑363‑5p

Hou

Wang

2020

Exp Ther Med

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The aim of the present study was to study the mechanism of the long non-coding (lnc)RNA MCM3AP-AS1 in the development of oral squamous cell carcinoma (OSCC). Patients with OSCC (n=36) volunteered to join the study, and their tumor/normal tissues were collected. MCM3AP-AS1 and microRNA (miR)-363-5p expression in tissues and cells was determined by reverse transcription-quantitative (RT-q)PCR. Following transfection, a CCK-8 assay and Transwell experiments were conducted to explore the effects of MCM3AP-AS1 on OSCC cell proliferation, migration and invasion. The interaction between MCM3AP-AS1 and miR-363-5p was detected by luciferase reporter gene assay. RT-qPCR analysis demonstrated significantly higher MCM3AP-AS1 expression in tumor tissues or OSCC cells compared with normal tissues or human oral keratinocytes cells (P<0.05). A high MCM3AP-AS1 level was associated with poor prognosis in OSCC patients (P<0.05 or P<0.01). Compared to the small interfering (si)-negative control (NC) group, OSCC cells of si-MCM3AP-AS1 group exhibited markedly lower optical density (at 450 nm) value and relative migration and invasion (P<0.05). miR-363-5p was directly inhibited by MCM3AP-AS1. OSCC cells of si-MCM3AP-AS1 + inhibitor-NC group exhibited clearly lower relative proliferation, migration and invasion compared with cells of si-NC + inhibitor-NC group and si-MCM3AP-AS1 + miR-363-5p inhibitor group (P<0.05). MCM3AP-AS1 promoted OSCC cells proliferation, migration and invasion by inhibiting miR-363-5p.

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“…Several studies have reported the close association of aberrant lncRNAs and miRNAs expressions to tumor development, including glioma [ 24 , 25 ]. lncRNA HAS2-AS1 promoted tumour progression in glioma by acting as a ceRNA [ 26 ]. HCG11 modulated glioma progression through its action on the miR-496/CPEB3 axis [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA DLGAP1-AS1 promotes the progression of glioma by regulating the miR-1297/EZH2 axis

Liu

Shi

et al. 2021

Aging

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Dysregulated lncRNAs have been implicated in a plethora of tumors, including glioma. One such oncogenic lncRNAs that has been reported in several cancers is the lncRNA DLGAP1 antisense RNA 1 (DLGAP1-AS1). This study seeks to characterize the expression of DLGAP1-AS1 in glioma tissues, which we found to be raised in both glioma samples and cell lines. Functional experiments revealed that DLGAP1-AS1 promoted in vitro glioma cell invasion, migration and proliferation. DLGAP1-AS1 was found to function as a miR-1297 sponge, based on information from luciferase reporter assays, RNA pull-down assays and publicly available online databases. miR-1297 was in turn found to functionally target EZH2. DLGAP1-AS1 modulated EZH2 expressions through miR-1297 sponging. Glioma progression appears to be supported DLGAP1-AS1 -promoted activation of the miR-1297/EZH2 axis. The components of this axis may function as therapeutic targets for glioma.

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Long noncoding RNA HAS2‐AS1 promotes tumor progression in glioblastoma via functioning as a competing endogenous RNA

Cited by 28 publications

References 40 publications

The transcription factor USF1 promotes glioma cell invasion and migration by activating lncRNA HAS2-AS1

The transcription factor USF1 promotes glioma cell invasion and migration by activating lncRNA HAS2-AS1

lncRNA MCM3AP‑AS1 promotes the development of oral squamous cell carcinoma by inhibiting miR‑363‑5p

Long noncoding RNA DLGAP1-AS1 promotes the progression of glioma by regulating the miR-1297/EZH2 axis

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