Long noncoding RNA HCP5 participates in premature ovarian insufficiency by transcriptionally regulating MSH5 and DNA damage repair via YB1

Wang, Xiaoyan; Zhang, Xinyue; Dang, Yujie; Li, Duan; Lü, Gang; Chan, Wai‐Yee; Leung, Peter C.K.; Zhao, Shidou; Qin, Yingying; Chen, Zi‐Jiang

doi:10.1093/nar/gkaa127

Cited by 94 publications

(69 citation statements)

References 51 publications

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“…Reduced HCP5 expression in GCs of biochemical POI patients contributed to the generation of dysfunctional GCs by modulating MSH5 transcription via the interaction with YB1. This study showed a novel epigenetic mechanism for POI pathogenesis [57].…”

Section: The Role Lncrnas In Poi Gcsmentioning

confidence: 65%

Long non-coding RNAs in ovarian granulosa cells

Chen

et al. 2020

J Ovarian Res

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Granulosa cells (GCs) are somatic cells surrounding oocytes within follicles and are essential for folliculogenesis. Pathological changes in GCs are found in several ovarian disorders. Recent reports have indicated that long noncoding RNAs (lncRNAs), which modulate gene expression via multiple mechanisms, are key regulators of the normal development of GCs, follicles, and ovaries. In addition, accumulating evidence has suggested that lncRNAs can be utilized as biomarkers for the diagnosis and prognosis of GC-related diseases, such as polycystic ovary syndrome (PCOS) and premature ovarian insufficiency (POI). Therefore, lncRNAs not only play a role in GCs that are involved in normal folliculogenesis, but they may also be considered as potential candidate biomarkers and therapeutic targets in GCs under pathological conditions. In the future, a detailed investigation of the in vivo delivery or targeting of lncRNAs and large-cohort-validation of the clinical applicability of lncRNAs is required.

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Section: The Role Lncrnas In Poi Gcsmentioning

confidence: 65%

Long non-coding RNAs in ovarian granulosa cells

Chen

et al. 2020

J Ovarian Res

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“…Using the Arraystar human lncRNA expression microarray v3.0, we previously 17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ). After initial screening by fold change (>2), length (<2,000 nt), raw signal intensity (>100), and without overlapping with coding transcripts, the top seven most differentially expressed lncRNAs ( FLJ26245 , RP11-626G11.1 , AC139099.6 , CTD-2335A18.1 , C8orf69 , LINC02690 , and RP11-773D16.1 ) were filtered out.…”

Section: Resultsmentioning

confidence: 99%

“… 13 , 14 Notably, functional lncRNAs have been shown to regulate gene expression and biological behavior in GCs, and therefore they are involved in female reproductive diseases such as polycystic ovary syndrome (PCOS), endometriosis, and POI. 15 , 16 , 17 Nevertheless, due to the low levels of expression and poor conservation of lncRNAs compared with protein-coding RNAs, the roles and causative mechanisms of lncRNAs in POI remain unclear.…”

Section: Introductionmentioning

confidence: 99%

lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1

Wang

Dang

et al. 2021

Molecular Therapy - Nucleic Acids

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Dysfunction of granulosa cells (GCs) leading to follicle atresia has been extensively studied as a major cause of premature ovarian insufficiency (POI), but the regulatory role of long non-coding RNAs (lncRNAs) in this process is still poorly understood. Here, we show that the lncRNA LINC02690 or GCAT1 (granulosa cell-associated transcript 1) is downregulated in GCs from patients with biochemical POI (bPOI), and we show a significant correlation between downregulated GCAT1 and serum levels of follicle-stimulating hormone and anti-Müllerian hormone. Downregulation of GCAT1 inhibited G1/S cell cycle progression and thus inhibited the proliferation of GCs. Mechanistically, we show that GCAT1 competes with cyclin-dependent kinase inhibitor 1B ( CDKN1B ) mRNA for polypyrimidine tract-binding protein 1 (PTBP1) binding, and thus decreased GCAT1 might promote PTBP1 binding to CDKN1B mRNA and thereby initiate CDKN1B protein (p27) translation. Together, our results suggest that downregulation of GCAT1 under conditions of bPOI inhibits the proliferation of GCs through PTBP1-dependent p27 regulation, thus suggesting a novel form of lncRNA-mediated epigenetic regulation of GC function that contributes to the pathogenesis of POI.

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“…Its essence is ovarian follicle depletion, and the resulting low estrogen levels in the ovary induce the occurrence and development of many other diseases [ 1 ]. Women under 40 years of age, who have hypomenorrhea or amenorrhea for at least 4 months, and whose follicle stimulating hormone (FSH) level are greater than 25 U/L, are defined as premature ovarian failure(POF )[ 2 , 3 ]. Because of the unclear pathogenesis, estrogen and progestogen replacement therapy are currently used to improve other diseases caused by low estrogen levels, however, adverse reactions are more frequent, and the effects are not good.…”

Section: Introductionmentioning

confidence: 99%

The role of Chito-oligosaccharide in regulating ovarian germ stem cells function and restoring ovarian function in chemotherapy mice

Huang

Zhu

et al. 2021

Reprod Biol Endocrinol

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In recent years, the discovery of ovarian germ stem cells (OGSCs) has provided a new research direction for the treatment of female infertility. The ovarian microenvironment affects the proliferation and differentiation of OGSCs, and immune cells and related cytokines are important components of the microenvironment. However, whether improving the ovarian microenvironment can regulate the proliferation of OGSCs and remodel ovarian function has not been reported. In this study, we chelated chito-oligosaccharide (COS) with fluorescein isothiocyanate (FITC) to track the distribution of COS in the body. COS was given to mice through the best route of administration, and the changes in ovarian and immune function were detected using assays of organ index, follicle counting, serum estrogen (E2) and anti-Mullerian hormone (AMH) levels, and the expression of IL-2 and TNF-α in the ovaries. We found that COS significantly increased the organ index of the ovary and immune organs, reduced the rate of follicular atresia, increased the levels of E2 and AMH hormones, and increased the protein expression of IL-2 and TNF-α in the ovary. Then, COS and OGSCs were co-cultured to observe the combination of COS and OGSCs, and measure the survival rate of OGSCs. With increasing time, the fluorescence intensity of cells gradually increased, and the cytokines IL-2 and TNF-α significantly promoted the proliferation of OGSCs. In conclusion, COS could significantly improve the ovarian and immune function of chemotherapy model mice, and improve the survival rate of OGSCs, which provided a preliminary blueprint for further exploring the mechanism of COS in protecting ovarian function.

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Long noncoding RNA HCP5 participates in premature ovarian insufficiency by transcriptionally regulating MSH5 and DNA damage repair via YB1

Cited by 94 publications

References 51 publications

Long non-coding RNAs in ovarian granulosa cells

Long non-coding RNAs in ovarian granulosa cells

lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1

The role of Chito-oligosaccharide in regulating ovarian germ stem cells function and restoring ovarian function in chemotherapy mice

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