Long noncoding RNA HEIH promotes melanoma cell proliferation, migration and invasion via inhibition of <i>miR-200b/a/429</i>

Zhao, Haiying; Xing, Guoping; Wang, Yingying; Luo, Zengxiang; Liu, Guoyan; Meng, Hui

doi:10.1042/bsr20170682

Cited by 51 publications

(53 citation statements)

References 42 publications

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“…The primers used were as follows: 5′-CCC AAGCTT ATCTTACGCCCGTCGCCCTGAG-3′ (forward) and 5′-CG GGATCC GACACGGGAAACAGGAGGATTTA-3′ (reverse). lncRNA HEIH overexpression vector pcDNA3.1-HEIH was constructed as previously described [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Long noncoding RNA ILF3-AS1 promotes cell proliferation, migration, and invasion via negatively regulating miR-200b/a/429 in melanoma

et al. 2017

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Melanoma is the most malignant skin cancer, which account for most of skin-cancer-related deaths. Long noncoding RNA (lncRNA) is a class of noncoding RNAs with crucial roles in many cancers. However, the roles of lncRNAs in melanoma have not been well studied. In the present study, using public available data and clinical tissues samples, we found that lncRNA ILF3-AS1 is up-regulated in melanoma tissues and cell lines, and correlated with poor prognosis of melanoma patients. Functional experiments showed that knockdown of ILF3-AS1 inhibits melanoma cell proliferation, migration, and invasion. Mechanistically, we found that ILF3-AS1 interacts with EZH2, promotes the binding of EZH2 to the miR-200b/a/429 promoter, and represses miR-200b/a/429 expression. The expression of ILF3-AS1 is negatively correlated with that of miR-200b/a/429 in melanoma tissues. Moreover, inhibition of miR-200b/a/429 abrogates the biological roles of ILF3-AS1 knockdown on melanoma cell proliferation, migration, and invasion. In conclusion, these results demonstrate that melanoma-upregulated lncRNA ILF3-AS1 promotes cell proliferation, migration, and invasion via negatively regulating miR-200b/a/429, and imply that ILF3-AS1 may be a potential prognostic biomarker and therapeutic target for melanoma.

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Section: Methodsmentioning

confidence: 99%

Long noncoding RNA ILF3-AS1 promotes cell proliferation, migration, and invasion via negatively regulating miR-200b/a/429 in melanoma

et al. 2017

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“…High expression in hepatocellular carcinoma long non‐coding RNA (HEIH), a highly expressed nuclear and cytoplasmic intergenic lncRNA in human melanoma lesion and cell lines A375, could promote melanoma cell proliferation, invasion and migration through repression of miR‐200b/a/429 transcription …”

Section: The Critical Role Of Lncrnas In Cutaneous Proliferation‐relamentioning

confidence: 99%

Long non‐coding RNAs in cutaneous biology and proliferative skin diseases: Advances and perspectives

et al. 2019

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Advances in transcriptome sequencing have revealed that the genome fraction largely encodes for thousands of non‐coding RNAs. Long non‐coding RNAs (lncRNAs), which are a class of non–protein‐coding RNAs longer than approximately 200 nucleotides in length, are emerging as key epigenetic regulators of gene expression recently. Intensive studies have characterized their crucial roles in cutaneous biology and diseases. In this review, we address the promotive or suppressive effects of lncRNAs on cutaneous physiological processes. Then, we focus on the pathogenic role of dysfunctional lncRNAs in a variety of proliferative skin diseases. These evidences suggest that lncRNAs have indispensable roles in the processes of skin biology. Additionally, lncRNAs might be promising biomarkers and therapeutic targets for cutaneous disorders.

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“…For instance, Chen et al (38) recently reported that lncRNA ILF3-AS1 promoted melanoma cell proliferation, migration, and invasion via negatively regulating miR-200b/a/429. Zhao et al (39) demonstrated that hepatocellular carcinoma upregulated EZH2-associated lncRNA enhanced the proliferation, migration and invasion of melanoma cells via inhibition of miR-200b/a/429. However, these studies did not investigate the downstream proteins of these axes.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of lncRNA‑UCA1 inhibits the proliferation and migration of melanoma cells through modulating the miR‑28‑5p/HOXB3 axis

et al. 2019

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Long non-coding RNA urothelial carcinoma-associated 1 (UCA1) functions as an oncogene in different human cancers, including melanoma. However, the molecular mechanism of UCA1 underlying melanoma progression still remains largely unknown. In the present study, reverse transcription quantitative polymerase chain reaction and western blot analyses were used to examine the mRNA and protein expression levels, respectively. Cell Counting Kit-8 and wound healing assays were conducted to study cell proliferation and migration, respectively. A luciferase reporter assay was used to confirm the targeting relationship. It was demonstrated that UCA1 expression was increased in melanoma tissues and cell lines. In addition, UCA1 expression was higher in melanoma tissues at stage III-IV than in tissues at stage I-II. Inhibition of UCA1 expression markedly reduced melanoma cell proliferation and migration. Further investigation revealed that UCA1 functioned in melanoma cells through directly binding with microRNA (miR)-28-5p. The expression of miR-28-5p was significantly reduced in melanoma tissues and had an inverse correlation with UCA1 expression. In addition, miR-28-5p expression was higher in melanoma tissues at advanced stages than in stage I-II tissues. Furthermore, homeobox (HOX)B3 was identified as a target gene of miR-28-5p in melanoma cells, and HOXB3 overexpression reversed the suppressive effects of UCA1 downregulation on melanoma cell proliferation and migration. Finally, HOXB3 was upregulated in melanoma tissues compared with its expression in adjacent tissues, and HOXB3 expression was increased in melanoma tissues at advanced stages. Taken together, the regulatory network of the UCA1/miR-28-5p/HOXB3 axis in melanoma was demonstrated for the first time in the present study, expanding the understanding of the molecular mechanism underlying melanoma progression. Future studies may further confirm the function of this signaling pathway in vivo.

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Long noncoding RNA HEIH promotes melanoma cell proliferation, migration and invasion via inhibition of miR-200b/a/429

Cited by 51 publications

References 42 publications

Long noncoding RNA ILF3-AS1 promotes cell proliferation, migration, and invasion via negatively regulating miR-200b/a/429 in melanoma

Long noncoding RNA ILF3-AS1 promotes cell proliferation, migration, and invasion via negatively regulating miR-200b/a/429 in melanoma

Long non‐coding RNAs in cutaneous biology and proliferative skin diseases: Advances and perspectives

Knockdown of lncRNA‑UCA1 inhibits the proliferation and migration of melanoma cells through modulating the miR‑28‑5p/HOXB3 axis

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