Long noncoding RNA HOXD-AS1 aggravates osteosarcoma carcinogenesis through epigenetically inhibiting p57 via EZH2

Gu, Wenfei; Zhang, Enfeng; Song, Lijun; Tu, Lei; Wang, Zhenbin; Tian, Feng; Aikenmu, Kahaer; Chu, Gao; Zhao, Jiang

doi:10.1016/j.biopha.2018.06.173

Cited by 30 publications

(23 citation statements)

References 21 publications

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“…Shi et al disclosed lncRNA PCAT6 played oncogenic roles in non‐small‐cell lung cancer by associating with EZH2 and subsequently suppressing LATS2 . Gu et al suggested lncRNA HOXD‐AS1 in complex with EZH2 aggravated osteosarcoma carcinogenesis through epigenetically inhibiting p57 . In line with this notion, our data exhibited LINC00630 specifically facilitated binding of EZH2 on BEX1 promoter and promoted its DNA methylation and epigenetic suppression.…”

Section: Discussionsupporting

confidence: 84%

Long noncoding RNA LINC00630 promotes radio‐resistance by regulating BEX1 gene methylation in colorectal cancer cells

et al. 2020

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This study aimed to analyze the relation between long noncoding RNA (lncRNA) LINCE00630 and radio‐resistance and elucidate the underlying mechanism. Relative expression of LINC00630, BEX1, and DNMT3B in colorectal cancer (CRC) cells and clinical samples was determined by real‐time PCR. Prognosis in respect of LINC00630 expression was analyzed by Kaplan–Meier survival curve. LINC00630 and BEX1 were specifically silenced by shRNAs. Cell viability and growth were analyzed by MTT and clonogenic assays, respectively. Cell apoptosis was measure by both caspase‐3 activity and flow cytometry. Association between EZH2 with LINC00630 and BEX1 promoter was determined by RNA immunoprecipitation and chromatin immunoprecipitation. BEX1 and DNMT3B proteins were quantified by Western blot. We demonstrated the elevated LINC00630 correlated with radio‐resistance and poorer prognosis in CRC. Knockdown of LINC00630 significantly improved the sensitivity of CRC cells to irradiation. Mechanistically, LINC00630 in complex with EZH2 negatively regulated BEX1 through promoter DNA methylation. BEX1 silencing greatly restored the cell viability and suppressed cell apoptosis, which were elicited by LINC00630 deficiency in response to irradiation. Our data uncovered the contribution of elevated LINC00630 to radio‐resistance in CRC, which was predominately mediated by epigenetically repressed BEX1.

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Section: Discussionsupporting

confidence: 84%

Long noncoding RNA LINC00630 promotes radio‐resistance by regulating BEX1 gene methylation in colorectal cancer cells

et al. 2020

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“…HOXD-AS1 silencing resulted in inhibition of tumor cell proliferation, induced cycle arrest in the G1/G0 phase in vitro, and suppressed tumor cell growth in vivo. It was confirmed that HOXD-AS1 could interact with homologous zest enhancer (EZH2) of the p57 gene promoter, inhibiting its tumor suppressive action, favoring OS oncogenesis (113). It has also been shown that expression of the TUG1 gene encoding lncRNA taurine 1 (TUG1) was significantly higher in tumor tissues than in adjacent normal bone tissues.…”

Section: Role Of Non-coding Rnasmentioning

confidence: 91%

Biology and pathogenesis of human osteosarcoma (Review)

Azevedo¹,

Fernandes

et al. 2019

Oncol Lett

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Osteosarcoma (OS) is a bone tumor of mesenchymal origin, most frequently occurring during the rapid growth phase of long bones, and usually located in the epiphyseal growth plates of the femur or the tibia. Its most common feature is genome disorganization, aneuploidy with chromosomal alterations, deregulation of tumor suppressor genes and of the cell cycle, and an absence of DNA repair. This suggests the involvement of surveillance failures, DNA repair or apoptosis control during osteogenesis, allowing the survival of cells which have undergone alterations during differentiation. Epigenetic events, including DNA methylation, histone modifications, nucleosome remodeling and expression of non-coding RNAs have been identified as possible risk factors for the tumor. It has been reported that p53 target genes or those genes that have their activity modulated by p53, in addition to other tumor suppressor genes, are silenced in OS-derived cell lines by hypermethylation of their promoters. In osteogenesis, osteoblasts are formed from pluripotent mesenchymal cells, with potential for self-renewal, proliferation and differentiation into various cell types. This involves complex signaling pathways and multiple factors. Any disturbance in this process can cause deregulation of the differentiation and proliferation of these cells, leading to the malignant phenotype. Therefore, the origin of OS seems to be multifactorial, involving the deregulation of differentiation of mesenchymal cells and tumor suppressor genes, activation of oncogenes, epigenetic events and the production of cytokines. Contents 1. Introduction 2. Biology of human OS 3. Role of differentiation of mesenchymal stem cells 4. Role of DNA changes 5. Role of deregulating the expression of tumor suppressor genes 6. Regulation of oncogene expression 7. Role of epigenetic mechanisms 8. Role of non-coding RNAs 9. Role of cytokines 10. Conclusion

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“…LncRNAs interfere gene expression in transcriptional or post‐transcriptional process by direct or indirect ways. For instance, lncRNA HOXD‐AS1 promotes osteosarcoma by recruiting the enhancer of zeste homolog 2 (EZH2), which binds to the promoter of P57 to inhibit p57 expression . LncRNA HCP5 functions as a ceRNA of miR‐22‐3p, miR‐186‐5p and miR‐216a‐5p, which activate ST6GAL2 and promote the development of follicular thyroid carcinoma …”

Section: Introductionmentioning

confidence: 99%

DANCR promotes HCC progression and regulates EMT by sponging miR‐27a‐3p via ROCK1/LIMK1/COFILIN1 pathway

Guo

Chen

et al. 2019

Cell Proliferation

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This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. AbstractObjectives: This research aims to verify that the long non-coding RNA differentiation antagonizing nonprotein coding RNA (LncRNA DANCR) could modulate the proliferation and metastasis of hepatocellular carcinoma (HCC), and it thus may work as a novel biomarker to render new orientation for early diagnosis and clinical therapy of HCC. Materials and methods:Firstly, qRT-PCR was used to detect the expression of genes including LncRNA DANCR and miR-27a-3p. Next, MTT assay, Ethynyldeoxyuridine (EdU) analysis and clone formation assay were used for investigating cell growth and proliferation. Meanwhile, transwell assay and wound healing assay were applied to evaluate the capacity of cell metastasis and motility, respectively. In addition, bioinformatic analysis and dual-luciferase reporter assay were applied to analyse molecular interaction. Next, we conducted immunofluorescence and Western blot for mechanic investigation. Last but not the least, xenograft tumours in nude mice were built by subcutaneously injecting Hep3B cells stably transfected with sh-NC and sh-DANCR to detect proliferation and SMMC-7721 cells stably transfected with sh-NC and sh-DANCR to investigate metastasis. Results:The results of qRT-PCR and bioinformatic analysis revealed the high expression of DANCR in HCC. DANCR accelerated proliferation and metastasis of HCC cells and the knockdown of DANCR had the opposite effect. Meanwhile, xenograft tumours in sh-DANCR group grow slower and have smaller volumes compared with negative control group. Next, the antineoplastic effect of miR-27a-3p on cell growth and motility of HCC was confirmed. In addition, we clarified that DANCR acted as a ceRNA to decoy miR-27a-3p via mediating ROCK1/LIMK1/COFILIN1 pathway. In the end, we validated that DANCR/miR-27a-3p axis regulates EMT progression by cell immunofluorescence and Western blot. Conclusions:In a word, DANCR promotes HCC development and induces EMT by decoying miR-27a-3p to regulate ROCK1/LIMK1/COFILIN1 pathway.

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Long noncoding RNA HOXD-AS1 aggravates osteosarcoma carcinogenesis through epigenetically inhibiting p57 via EZH2

Cited by 30 publications

References 21 publications

Long noncoding RNA LINC00630 promotes radio‐resistance by regulating BEX1 gene methylation in colorectal cancer cells

Long noncoding RNA LINC00630 promotes radio‐resistance by regulating BEX1 gene methylation in colorectal cancer cells

Biology and pathogenesis of human osteosarcoma (Review)

DANCR promotes HCC progression and regulates EMT by sponging miR‐27a‐3p via ROCK1/LIMK1/COFILIN1 pathway

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