Long noncoding RNA ILF3-AS1 promotes cell proliferation, migration, and invasion via negatively regulating miR-200b/a/429 in melanoma

Abstract

Melanoma is the most malignant skin cancer, which account for most of skin-cancer-related deaths. Long noncoding RNA (lncRNA) is a class of noncoding RNAs with crucial roles in many cancers. However, the roles of lncRNAs in melanoma have not been well studied. In the present study, using public available data and clinical tissues samples, we found that lncRNA ILF3-AS1 is up-regulated in melanoma tissues and cell lines, and correlated with poor prognosis of melanoma patients. Functional experiments showed that knockdown of ILF3-AS1 inhibits melanoma cell proliferation, migration, and invasion. Mechanistically, we found that ILF3-AS1 interacts with EZH2, promotes the binding of EZH2 to the miR-200b/a/429 promoter, and represses miR-200b/a/429 expression. The expression of ILF3-AS1 is negatively correlated with that of miR-200b/a/429 in melanoma tissues. Moreover, inhibition of miR-200b/a/429 abrogates the biological roles of ILF3-AS1 knockdown on melanoma cell proliferation, migration, and invasion. In conclusion, these results demonstrate that melanoma-upregulated lncRNA ILF3-AS1 promotes cell proliferation, migration, and invasion via negatively regulating miR-200b/a/429, and imply that ILF3-AS1 may be a potential prognostic biomarker and therapeutic target for melanoma.

Long noncoding RNA NEAT1‐modulated miR‐506 regulates gastric cancer development through targeting STAT3

“…Therefore, we speculated that the NEAT1/miR-506/STAT3 axis was involved in GC development in vitro. 26 LncRNA SNHG17 is able to promote cell proliferation by acting as an unfavorable prognostic indicator and epigenetically inhibiting P57 in colorectal cancer. 21 They are manifested to be involved in biological processes.…”

Long non‑coding RNA TTN‑AS1 promotes breast cancer cell migration and invasion via sponging miR‑140‑5p

“…By targeting miR-873, lncRNA NRF modulates programmed necrosis and myocardial injury during ischemia and reperfusion (8). Through negatively regulating miR-200b/a/429, lncRNA ILF3-AS1 enhances cell proliferation, cell migration and invasion in melanoma (9). By acting as a molecular sponge for miR-200s, depletion of lncRNA ZEB1-AS1 significantly suppresses cell proliferation and cell migration in osteosarcoma (10).…”

Long noncoding RNA TTN-AS1 promotes breast cancer cell migration and invasion via sponging miR-140-5p

“…By targeting miR-873, lncRNA NRF modulates programmed necrosis and myocardial injury during ischemia and reperfusion [8] . Through negatively regulating miR-200b/a/429, lncRNA ILF3-AS1 enhances cell proliferation, cell migration and invasion in melanoma [9] . By acting as a molecular sponge for miR-200s, depletion of lncRNA ZEB1-AS1 significantly suppresses cell proliferation and call migration in osteosarcoma [10] .…”