Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression

Abstract

Accumulating evidence has suggested that long noncoding RNAs (lncRNAs) exert crucial modulation roles in the biological behaviors of multiple malignancies. Nonetheless, the specific function of lncRNA LINC00284 in papillary thyroid cancer (PTC) remains not fully understood. The objective of this research was to explore the influence of LINC00284 in PTC and elucidate its potential mechanism. The Cancer Genome Atlas (TCGA), gene expression omnibus (GEO) datasets were used to analyze LINC00284 expression differences in thyroid cancer and normal samples, followed by the verification of qRT-PCR in our own PTC and adjacent non-tumor tissues. The impacts of LINC00284 on PTC cell growth were detected in vitro via CCK-8, colony formation, EdU assays, and in vivo via a xenograft tumor model. Bioinformatics analyses and biological experiments were conducted to illuminate the molecular mechanism. We found that LINC00284 expression was remarkably increased in PTC tissues and its overexpression was closely correlated with larger tumor size. In addition, silencing LINC00284 could effectively attenuate PTC cell proliferation, induce apoptosis and G1 arrest in vitro, as well as suppress tumorigenesis in mouse xenografts. Mechanistic investigations showed that LINC00284 acted as a competing endogenous RNA (ceRNA) for miR-3127-5p, thus resulting in the disinhibition of its endogenous target E2F7. In short, our findings indicated that LINC00284–miR-3127-5p–E2F7 axis exerted oncogenic properties in PTC and may offer a new promising target for the diagnosis and therapy of PTC.

Genomic Instability-Related LncRNA Signature Predicts the Prognosis and Highlights LINC01614 Is a Tumor Microenvironment-Related Oncogenic lncRNA of Papillary Thyroid Carcinoma

“…FOXD2-AS1 was recognized as a molecular biomarker and participated in chemo-resistance, malignant proliferation, invasion, and immunosuppression (46,(51)(52)(53)(54)(55). Recently, a study demonstrated that overexpression of FOXD2-AS1 was associated with poor clinical outcomes in PTC (57)(58)(59). GI-associated ceRNA regulatory network should be evaluated in the future studies.…”

“…Furthermore, we observed that GILS could identify the BRAF mutation status and share commonalities with clinical prognostic parameters. In addition, lncRNA, mRNA & miRNA likely work together to form a competing endogenous RNA (ceRNA) regulatory network, which could be used as prognostic markers in cancers ( 57 – 59 ). GI-associated ceRNA regulatory network should be evaluated in the future studies.…”

Development of an Inflammation-Related lncRNA-miRNA-mRNA Network Based on Competing Endogenous RNA in Breast Cancer at Single-Cell Resolution

“…By combination of correlation analysis, expression analysis and survival analysis, miR-3127-5p and miR-2114-3p were selected as the two most potential downstream binding miRNAs of LRRC75A-AS1 in breast cancer. Both of the two miRNAs have been reported to be involved in initiation and progression of human malignancies, including osteosarcoma (Wang et al, 2021), papillary thyroid cancer (Zhou et al, 2021), colorectal cancer (Ma et al, 2021) and ovarian cancer (Yu Z et al, 2020).…”

MicroRNA-22-3p targeted regulating transcription factor 7-like 2 (TCF7L2) constrains the Wnt/β-catenin pathway and malignant behavior in osteosarcoma

“…The long and short diameters of tumors in mice were recorded weekly with a vernier caliper. Volume = (long diameter × short diameter)2 × 0.5 [ 24 ]. Four weeks later, the mice were anesthetized with pentobarbital sodium (30 mg/kg) and the tumors were weighed.…”